Seasonal variation of triterpenes and phenolic compounds in australian <i>Centella asiatica</i> (L.) Urb

Alqahtani, Ali S.; Tongkao-on, Wannit; Li, Kong M.; Razmovski-Naumovski, Valentina; Chan, Kelvin; Li, George Q.

doi:10.1002/pca.2578

Cited by 93 publications

(60 citation statements)

References 24 publications

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“…Similar studies have been reported on C. asiatica wherein it was shown to accumulate major phytoconstituents in the months of summer season (Alqahtani et al, 2015). Moreover, studies on Ribes nigrum and 'Zonouz' peel have shown that harvesting time and leaf position have a profound effect on their phenolic contents and (Michael et al, 2015 andHallman et al, 2013).…”

Section: Resultssupporting

confidence: 63%

“…A wide range of chemical compounds including cardiac glycosides, fl avonoids, terpenoids, alkaloids and resins have been isolated from this plant (Singh et al 2014). Recent studies have shown that, C. asiatica accumulates major phytoconstituents in the months of summer season (Alqahtani et al, 2015). Moreover, studies on Ribes nigrum and 'Zonouz' peel have shown that harvesting time and leaf position have a profound effect on their phenolic contents, (Hallman et al, 2013 andMichael et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Temporal variation in the quantitative estimation of total phenolic and fl avonoid contents of two species ofCalotropis

Najar¹,

Khare²,

Jain³

2016

Biosci. Biotech. Res. Comm

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Phenols and fl avonoids present in medicinal plants are considered to be among the most important bioactive components. Calotropis procera and Calotropis gigantea are much alike medicinal plants with a wide range of bioactivity. The present study was aimed to evaluate the effect of time on total phenolic content (TPC) and total fl avonoid content (TFC) in the methanolic extracts of leaf and fl ower tissues of C. procera and C. gigantea. TPC and TFC were estimated by spectrophotometric method using gallic acid and quercetin as respective standards. The samples were collected in morning, afternoon and evening session's. Signifi cant variation in TFC and TPC levels was observed in between the selected species. C. procera presented highest TPC in leaves harvested in afternoon (20.10 mg/gm) and highest TFC in fl owers collected in evening (36.755 mg/gm). In conclusion, the present investigation demonstrates that there is signifi cant effect of harvesting time of different tissues of C. procera and C. gigantea on their TPC and TFC contents.

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Section: Resultssupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Temporal variation in the quantitative estimation of total phenolic and fl avonoid contents of two species ofCalotropis

Najar¹,

Khare²,

Jain³

2016

Biosci. Biotech. Res. Comm

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“…This extract contains a high proportion of glycosides than the aglycones, in which madecassoside is the highest component. Several researchers were able to detect these triterpenes within the wavelength range of 200-220 using a gradient system comprising water and acetonitrile [28][29][30]. The cytotoxic effect of RECA at a concentration ranging from 3.91 to 1000 µg/mL was investigated on neuroblastoma cells (SH-SY5Y) and murine microglia cells (RAW 264.7) by using MTT assay.…”

Section: Quantitative High-performance Liquid Chromatography (Hplc) Amentioning

confidence: 99%

Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo

et al. 2020

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Centella asiatica (C. asiatica) is one of the medicinal plants that has been reported to exert comprehensive neuroprotection in vitro and in vivo. In view of this, the present study was performed to investigate the effect of ethanolic extract of C. asiatica, designated as raw-extract of C. asiatica (RECA) in reducing the acetylcholinesterase (AChE), inflammations, and oxidative stress activities via both in vitro (SH-SY5Y and RAW 264.7 cells) and in vivo (Sprague Dawley rats). Quantitative high-performance liquid chromatography analysis reveals that RECA contains a significantly high proportion of glycosides than the aglycones with madecassoside as the highest component, followed by asiaticoside. Treatment of SH-SY5Y cells with RECA significantly reduced the AChE activity in a concentration-dependent manner with an IC50 value of 31.09 ± 10.07 µg/mL. Furthermore, the anti-inflammatory and antioxidant effects of RECA were evaluated by lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. Our results elucidated that treatment with RECA significantly suppressed the level of pro-inflammatory cytokine/mediators and oxidative stress released in a concentration-dependent manner. Interestingly, these patterns of inhibition were consistent as observed in the LPS-induced neuroinflammation Sprague Dawley rats’ model. The highest concentration used in the two models presented the most significant results. Herein, our findings strongly suggest that RECA may offer therapeutic potential for the treatment of Alzheimer’s disease through inhibiting the AChE, inflammation, and oxidative stress activities.

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“…Then the sample solution was filtered through a 0.45‐μm syringe filter, and 10 μL of the filtrate was injected into the HPLC system for quantification analysis. The quantitation of each compound was expressed as grams per 100 grams of dried weight (g/100 g DW) of the seed shell …”

Section: Methodsmentioning

confidence: 99%

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

Zhang

Liu

Gao

et al. 2019

Phytochemical Analysis

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Introduction The Paeonia ostii T. Hong & J. X. Zhang seed shell, characterised by a high content of oligostilbenes, is one of the two most important by‐products in the preparation of seed oil. Oligostilbenes are considered characteristic constituents of the genus Paeonia, and can be used in fingerprinting to determine the geographical origin and the quality of raw materials. Objective To develop and optimise a simple and reproducible high‐performance liquid chromatography diode array detection (HPLC‐DAD) method for the simultaneous determination of seven oligostilbenes in P. ostii seed shell from different geographical areas, and to associate the cultivation area. Methodology A validated HPLC method coupled with a DAD detector was performed for the detection and determination of target compounds in the samples. Optimal chromatographic conditions were achieved using an Agilent Zorbax Eclipse SB‐AQ‐C18 column and a gradient elution with acetonitrile and potassium dihydrogen phosphate solution. Results The proposed quantitative method showed appropriate accuracy and precision, and was successfully applied to the routine analysis of seven oligostilbenes and the quality evaluation of 50 P. ostii seed shell samples. There were significant differences between the contents of the seven oligostilbenes in different samples (P < 0.01). Conclusion The results demonstrated that the oligostilbenes were main secondary metabolites in the P. ostii seed shells, and the content of seven components in P. ostii seed shells sourced from different cultivation areas in China was different.

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Seasonal variation of triterpenes and phenolic compounds in australian Centella asiatica (L.) Urb

Abstract: The results indicate that harvesting C. asiatica in summer returns the highest yield of the target triterpenoids, kaempferol and chlorogenic acid.

Cited by 93 publications

References 24 publications

Temporal variation in the quantitative estimation of total phenolic and fl avonoid contents of two species ofCalotropis

Temporal variation in the quantitative estimation of total phenolic and fl avonoid contents of two species ofCalotropis

Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo

Profiling and simultaneous quantitative determination of oligostilbenes in Paeonia ostii seed shell from different geographical areas in China and their comparative evaluation

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