Phenols and fl avonoids present in medicinal plants are considered to be among the most important bioactive components. Calotropis procera and Calotropis gigantea are much alike medicinal plants with a wide range of bioactivity. The present study was aimed to evaluate the effect of time on total phenolic content (TPC) and total fl avonoid content (TFC) in the methanolic extracts of leaf and fl ower tissues of C. procera and C. gigantea. TPC and TFC were estimated by spectrophotometric method using gallic acid and quercetin as respective standards. The samples were collected in morning, afternoon and evening session's. Signifi cant variation in TFC and TPC levels was observed in between the selected species. C. procera presented highest TPC in leaves harvested in afternoon (20.10 mg/gm) and highest TFC in fl owers collected in evening (36.755 mg/gm). In conclusion, the present investigation demonstrates that there is signifi cant effect of harvesting time of different tissues of C. procera and C. gigantea on their TPC and TFC contents.
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