Seasonal variation and tissues specificity of endophytic fungi of Dillenia indica l. and their extracellular enzymatic activity

Kumar, Vijay; Prasher, I. B.

doi:10.1007/s00203-022-02933-7

Cited by 9 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result was similar to the endophytic fungi diversity in Croton chinensis (43). Similar research was also observed in Dillenia indica where the frequency of endophytic fungi was showing higher in the leaf tissue (8). Furthermore, tissue specificity of fungal endophytes from T. arjuna was exhibited after the analysis of diversity indices.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Endophytic fungal diversity in Terminalia arjuna (Roxb.) Wight & Arn. of Tripura, Northeast India at different sampling sites and plant organs

Tripura,

Debbarma,

Paul

et al. 2024

Plant Sci. Today

View full text Add to dashboard Cite

Endophytic fungi are ubiquitous in plant kingdom and play a vital role in balancing the microenvironments within the host plants. Fungal endophytes isolated from ethno-botanically important plants were the source of several secondary metabolites with potential biological activities. The present study has documented the variability of culturable endophytic fungi from Terminalia arjuna (Roxb.) Wight & Arn. (Combretaceae), a widely used medicinal plant of Tripura, North-east, India. A total of 613 fungal strains were isolated from 720 tissue segments viz. leaves, bark and root of T. arjuna. The highest numbers of endophytic fungal isolates were found to be colonizing in leaves (257) followed by barks (182) and the least number of isolates were obtained from roots (174). The fungal isolates were classified into 27 individual fungal strains based on morphological and microscopic features. A total of 9 endophytic fungal strains were identified using the nuclear ribosomal DNA internal transcribe sequence and were subjected to phylogeny analysis. Most of the identified morphotypes belonged to phylum Ascomycota. Among all the isolates, Diaporthe sp., Fusarium sp., Colletotrichum sp., Penicillium sp. were the most abundant fungal isolates from T. arjuna. The fungal orders namely Sordariomycetes was the most prevalent followed by Eurotiomycetes and Dothideomycetes. Colonization rate, isolation rate, colonization frequency and relative frequency results suggest that leaf segments harbors maximum endophytic fungi assemblage compared to bark and root tissues. The analysis of Shannon (H'), Simpson (1-D), Fisher alpha (a) and Brillouin (HB) indices had significant difference with locations and tissue type. The host plant harbor unique endophytic community composition in studied sampling locations and their colonization varied between the inoculated vegetative parts of the plant. As no such study has been undertaken in the North- eastern part of India, this pioneer study may enable to unearth novel fungal endophytes that might have beneficial role in plant growth promotion, stress tolerance etc.

show abstract

Section: Discussionsupporting

confidence: 87%

“…These substances have been utilized in industry, agriculture and medicines in recent time (7). Some of the biological activities of secondary metabolites produced by endophytic fungi are antioxidant, antibacterial, antimicrobial, antifungal, antiviral, anticancer and immunosuppressive capabilities (8).…”

Section: Introductionmentioning

confidence: 99%

Endophytic fungal diversity in Terminalia arjuna (Roxb.) Wight & Arn. of Tripura, Northeast India at different sampling sites and plant organs

Tripura,

Debbarma,

Paul

et al. 2024

Plant Sci. Today

View full text Add to dashboard Cite

show abstract

“…A total of 14 endophytic fungi were isolated from the host plant, among which 11 fungal endophytes were identified using morphological-macroscopic characteristics till genus level whereas, 4 were identified till species using molecular techniques. In the current study, all the isolated endophytic fungal taxa belong to the phylum Ascomycota, which further grouped into 3 classes (Sordariomycetes, Dothideomycetes, and Eurotiomycetes) were similar to the findings of Gopane et al 2021;Goveas et al, 2011;Kumar & Prasher., 2022, in their studies on Coscinium fenestratum, Nigella sativa and Dillenia indica.…”

Section: Discussionsupporting

confidence: 81%

Seasonal variation and diversity of endophytic fungi in Chromolaena odorata (L.) King and Robinson, an invasive alien weed of Tripura, Northeast, India

Debbarma,

Tripura,

Paul

et al. 2024

Asian J Pharm Pharmacol

View full text Add to dashboard Cite

Endophytes are micro-organisms (fungi or bacteria) that spend the whole or part of their life cycle, invading the living tissues of host plants without developing symptoms of infection and even disease (Materatski et al., 2018;Wilson, 1995). The fungal endophytic community composition is highly influenced by both abiotic and biotic factors such as temperature, humidity, location, plant physiology, plant host genotype, tissue type, pathogen infections, and anthropogenic influences (Cevallos et al., 2017; Christian et al., 2016). Several studies mentioned that properties of soil, availability of nutrients, root exudation, and specific climatic conditions, e.g., temperature and precipitation, are also decisive drivers of endophytic microfungal communities in plant roots (Pecundo et al., 2021). Endophytic fungi are highly distributed among plants and it is estimated that each plant species harbors not less than one endophytic fungal species, although, this number may vary based on the host species subjected to isolation (Arnold et al., 2000; Correia et al., 2017). Fungal endophytes play a vital role in the successful population establishment of alien invasive species in newly invaded areas outcompeting native species (Clay et al., 2016; Seifert et al., 2009). The association of particular microbes with non-native invasive species poses severe threats to native plant competitors (Clay et al., 2016;Garnica et al., 2022). These microbes have the potential to alter the diversity and composition of entire plant Seasonal variation and diversity of endophytic fungi in Chromolaena odorata (L.) King and Robinson, an invasive alien weed of Tripura,

show abstract

“…In addition, the relative abundances of the species were calculated and the similarity between the fungal communities was estimated using Sorensen’s index [ 92 ]. The species diversity of different hosts in each sampling site was calculated by the Shannon diversity (H’), Margalef diversity (d), and the Pielou evenness indices (J’), H’ = -∑ (Pi ln [Pi]), Pi = ni/N, ni = number of individuals of the species i, and N = total number of individuals of all species; d = S-1/Log N, where S is the number of species and N is the total number of specimens in the sample; J’ = H’/Log (S), where H’ is the value obtained by the Shannon index and S is species richness [ 26 , 93 , 94 ].…”

Section: Methodsmentioning

confidence: 99%

Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens

Karunarathna

Hyde

et al. 2022

JoF

View full text Add to dashboard Cite

Coffee endophytes have been studied for almost 74 years, and several studies have demonstrated coffee-endophytic fungi with antibacterial and antifungal potential for human and plant pathogens. In this study, we isolated and identified a total of 235 strains of endophytic fungi from coffee leaf tissues collected in four coffee plantations in Pu’er city, Yunnan province, China. Molecular identification was carried out using maximum likelihood phylogenetic analysis of nuclear ribosomal internal transcribed spacer (ITS1-5.8S rDNA-ITS2) sequences, while the colonization rate and the isolation frequency were also calculated. Two pathogenic fungi (Alternaria alternata and Penicillium digitatum) and two pathogenic bacteria (Pseudomonas syringae and Salmonella enterica subsp. enterica) were used for screening the antagonistic activities of 61 strains of coffee-endophytic fungi by a dual-culture test assay while maximum likelihood phylogenetic analysis confirmed their natural classification. This is the first study of coffee-leaf-endophytic fungal diversity in China, and the results revealed that coffee-endophytic fungi from this study belong to the Ascomycota, distributed among two classes, 10 orders, and 17 families. Concurrently, endophytic fungi isolates distributed in Arthrinium, Biscogniauxia, Daldinia, Diaporthe, and Nigrospora showed strong antagonistic activities against the pathogens. For the pathogens Alternaria alternata and Pseudomonas syringae, Nigrospora XCE-7 showed the best inhibitory effects with inhibition rates of 71.76% and 61.11%, respectively. For the pathogen Penicillium digitatum, Daldinia ME-9 showed the best inhibitory effect with a 74.67% inhibition rate, while Biscogniauxia PTE-7 and Daldinia T5E-1-3 showed the best inhibitory effect with a rate of 60.42% against the pathogen Salmonella enterica subsp. enterica. Overall, our study shows the diversity of coffee endophytes in four coffee-growing areas in Pu’er city, Yunnan province, China, and their potential use as biological control agents against two fungal and two bacterial pathogens.

show abstract

Seasonal variation and tissues specificity of endophytic fungi of Dillenia indica l. and their extracellular enzymatic activity

Cited by 9 publications

References 48 publications

Endophytic fungal diversity in Terminalia arjuna (Roxb.) Wight & Arn. of Tripura, Northeast India at different sampling sites and plant organs

Endophytic fungal diversity in Terminalia arjuna (Roxb.) Wight & Arn. of Tripura, Northeast India at different sampling sites and plant organs

Seasonal variation and diversity of endophytic fungi in Chromolaena odorata (L.) King and Robinson, an invasive alien weed of Tripura, Northeast, India

Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens

Contact Info

Product

Resources

About