Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens

Li, Lu; Karunarathna, Samantha C.; Hyde, Kevin D.; Suwannarach, Nakarin; Elgorban, Abdallah M.; Stephenson, Steven L.; Al-Rejaie, Salim S.; Jayawardena, Ruvishika S.; Tibpromma, Saowaluck

doi:10.3390/jof8070698

Cited by 11 publications

(9 citation statements)

References 97 publications

(127 reference statements)

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“…Some Nigrospora species can act as an antagonist against fungal and bacterial plant pathogens [57,58]. Phomalactone from Nigrospora sphaerica exhibits a broad spectrum of antimicrobial activity against human and phytopathogenic bacteria and fungi [59], such as Phytophthora infestans (Mont.)…”

Section: Discussionmentioning

confidence: 99%

First Report of Nigrospora Species Causing Leaf Spot on Olive (Olea europaea L.)

Petrović,

Vrandečić,

Ćosić

et al. 2023

Horticulturae

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Leaf spot symptoms were spotted in two olive orchards in Istria and in Kvarner Gulf, Croatia. Fungal species from three representative isolates (P13 LECIII, R18 BI, JA20 NP) have been morphologically characterized based on the colony and conidial characteristics. Several techniques were performed for inducing the sporulation of the JA20 NP isolate. Only PDA + banana medium was successful. PCR was conducted for ITS, TUB, and EF1α gene regions. Phylogenetic analyses were performed using internal transcribed spacer, beta-tubulin, and translation elongation factor 1-alpha sequence data. Three types of tests were conducted: a pathogenicity test on detached leaves, on detached and scratched leaves, and on olive seedlings. Ultimately, from the morphological characterizations, DNA sequence analysis of ITS, TUB, and EF1α gene regions, and phylogenetic analysis, these species were identified as Nigrospora gorlenkoana Novobr., Nigrospora osmanthi Mei Wang & L. Cai, and Nigrospora philosophiae-doctoris M. Raza, Qian Chen & L. Cai. This is the first report of Nigrospora species causing leaf spot on olive trees and the first report of Nigrospora philosophiae-doctoris as a plant pathogen. Fungal leaf diseases in conditions that are favorable for infection and disease development can lead to a decrease in the yield and olive oil quality. Therefore, it is necessary to conduct further research and the monitoring of fungal leaf diseases.

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Section: Discussionmentioning

confidence: 99%

First Report of Nigrospora Species Causing Leaf Spot on Olive (Olea europaea L.)

Petrović,

Vrandečić,

Ćosić

et al. 2023

Horticulturae

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“…Four fungal isolates (YH-4, YH-10, YH-16, and YH-26) were evaluated for antifungal activity against pathogens ( Alternaria alternata , Candida albicans , Fusarium oxysporum , Botrytis cinerea , and Pythium ultimum ) that were kindly obtained from the Department of Botany and Microbiology, University of King Saud, using a dual culture approach [ 26 ]. Thus, pathogens were inoculated in the center of PDA plates, and representative endophytic isolates (5 mm) were seeded on three corners of PDA plates for analysis.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant, Cytotoxic, and DNA Damage Protection Activities of Endophytic Fungus Pestalotiopsis neglecta Isolated from Ziziphus spina-christi Medicinal Plant

Almustafa

Yehia

2023

Microorganisms

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Fungal endophytes are friendly microorganisms that colonize plants and are important in the interactions between plants and their environment. They generate valuable secondary metabolites that are valuable to both plants and humans. Endophytic fungi with bioactivities were isolated from the leaves of the medicinal plant Ziziphus spina-christi. An efficient isolate was selected and identified as Pestalotiopsis neglecta based on nucleotide sequencing of the internal transcribed spacer region (ITS 1-5.8S-ITS 2) of the 18S rRNA gene (NCBI accession number OP529850); the 564 bp had 99 to 100% similarity with P. neglecta MH860161.1, AY682935.1, KP689121.1, and MG572407.1, according to the BLASTn analysis, following preliminary phytochemical and antifungal screening. The biological activities of this fungus’ crude ethyl acetate (EtOAc) extract were assessed. With an efficient radical scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl and an IC50 value of 36.6 µg mL−1, P. neglecta extract has shown its potential as an antioxidant. Moreover, it displayed notable cytotoxic effects against MCF-7 (breast carcinoma, IC50 = 22.4 µg mL−1), HeLa (cervical carcinoma, IC50 = 28.9 µg mL−1) and HepG-2 (liver carcinoma, IC50 = 28.9 µg mL−1). At 10 µg mL−1, EtOAc demonstrated significant DNA protection against hydroxyl radical-induced damage. Based on FT-IR and GC-MS spectral analysis, it was detected that the EtOAc of P. neglecta product contains multiple bioactive functional groups. Subsequently, this validated the features of major different potent compounds; tolycaine, 1H-pyrazol, 1,3,5-trimethyl-, eugenol, 2,5-cyclohexadiene-1,4-dione, 2,6-bis(1,1-dimethyl), and bis(2-ethylhexyl) phthalate. Since these compounds are biologically relevant in various aspects, and distinct biological activities of fungal extract were acceptable in vitro, this suggests that endophytic fungus P. neglecta may be a viable source of bioactive natural products. This could be a good starting point for pharmaceutical applications.

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“…strain PTE‐7 and Daldinia sp. strain T5E‐1‐3, have an antagonistic effect against S. enterica with an inhibition rate of approximately 60%, tested by dual‐culture assays in Petri dishes (L. Lu, Karunarathna, et al, 2022). In contrast, Glomus intraradices , an arbuscular mycorrhizal fungus, did not show an inhibitory effect either the survival or the internalization of E. coli O157:H7, S. newport , S. typhimurium , S. saintpaul , and S. montevideo in romaine lettuce sprouts and in the soil, in none of the evaluated times (1, 8, 15, and 22 days).…”

Section: Biological Control Agents For Human Pathogens On Producementioning

confidence: 99%

Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

Barba‐León,

Arista‐Regalado,

Mancilla‐Becerra

et al. 2024

Journal of Food Safety

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Foodborne pathogens can cause gastrointestinal infections in consumers and in some cases can even lead to outbreaks. In the last decade, it has been observed that some zoonotic pathogenic bacteria can use plants as secondary hosts. Contamination with foodborne bacteria becomes relevant in foods that are regularly eaten raw, such as lettuce, cilantro, fenugreek, rocket leaves, basil, and so forth, and some fruits such as tomatoes, melons, and green peppers; because the elimination of these pathogenic bacteria is difficult to achieve with conventional sanitization processes. Contamination of produce can occur throughout the entire production chain. In farmlands, pathogenic bacteria can contaminate the seed, mainly when contaminated water is used for irrigation. Later, bacteria can reach other plant tissues such as the stems, leaves, and fruits. Another form of contamination is when the produce is in contact with feces from domestic, production, or wild animals. Additionally, poor handling practices during harvest, packaging, distribution, and sale can contaminate produce. Studies have shown that foodborne pathogens can adhere to produce, sometimes forming a biofilm, and can also be internalized into the plant or fruit, which protects them from sanitation processes. For this reason, in this text we address three biocontrol strategies such as bacteria, lytic bacteriophages, and some fungi, as an alternative approach for the control of both foodborne and plant pathogens. Additionally, the use of these biological agents can represent an advantage for the development of the plant, making them a good strategy to favor yield.

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Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens

Cited by 11 publications

References 97 publications

First Report of Nigrospora Species Causing Leaf Spot on Olive (Olea europaea L.)

First Report of Nigrospora Species Causing Leaf Spot on Olive (Olea europaea L.)

Antioxidant, Cytotoxic, and DNA Damage Protection Activities of Endophytic Fungus Pestalotiopsis neglecta Isolated from Ziziphus spina-christi Medicinal Plant

Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

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