Search forMycobacterium aviumSubspeciesparatuberculosisAntigens for the Diagnosis of Paratuberculosis

Abstract

The aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PTB (n = 25), healthy animals (n = 10), and animals experimentally infected with M. bovis (n = 8). This initial screening allowed us to select seven antigens: MAP 2513, MAP 1693, MAP 2020, MAP 0038, MAP 1272, MAP 0209c, and MAP 0210c, which reacted with sera from animals with PTB and showed little cross-reactivity with sera from healthy animals and animals experimentally infected with M. bovis. The second step was to evaluate the antigen cocktail of these seven antigens by ELISA. For this evaluation, we used sera from animals with PTB (n = 25), healthy animals (n = 26), and animals experimentally infected with M. bovis (n = 17). Using ELISA, the cocktail of the seven selected MAP antigens reacted with sera from 18 of the 25 animals with PTB and did not exhibit cross-reactivity with healthy animals and only low reactivity with animals with bovine tuberculosis. The combined application of these antigens could form part of a test which may help in the diagnosis of PTB.

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

“…Normal architecture of both small intestinal and associated lymph nodes was strongly altered in diffuse lesions that were specifically named according to the predominant cell-type infiltrate: histiocytic, lymphoplasmacytic or intermediate. (Mon et al, 2012), was used for detecting specif ic antibodies against MAP in plasma samples according to the manufacturer's instructions. In each assay, negative and positive controls included in the kits were tested in duplicate.…”

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

“…Each of these diagnostic tests has a variable sensitivity and specificity which is crucial for both the initial screening test and confirmatory assays. All diagnostic methods are fraught with difficulties that have impeded the control and eradication of pTB (Wood et al 1989;Collins 1996;Pavlas et al 1997;Cho et al 2007;Leroy et al 2007;Martinson et al 2008;Shin et al 2008;Fern andez-Silva et al 2011;Mon et al 2012;Wadhwa et al 2012;Singh 2015). In chronic infection like pTB, local strains are key to the development of efficient diagnostic tests and vaccines; therefore, the use of commercial diagnostic kits globally has led to underreporting of the infection whenever used.…”

“…Huge and diverse natural resources of domestic livestock species in terms of breeds and strains are found on the Earth. Such polymorphism and inherent capacity of individual animals to resist MAP infection can form the basis of breeding of animals for disease resistance as an alternative tool for building the 'herd immunity' (Romain et al 1993;Willemsen et al 2006;Cho et al 2007;Roupie et al 2008;Gioffre et al 2009;Souza et al 2011;Mon et al 2012;Roupie et al 2012;Carlos et al 2015). In addition, secretory proteins are either present in very scanty (low) amount or absent in vaccines, which are as a result unlikely to induce antibodies against the secretory proteins.…”

Diva technology: indispensable tool for the control of Johne’s disease

“…Putative invasin, NlpC/P60 superfamily Tested for antigenicity by Mon et al (2012) Rosseels et al (2006) 37. Ag85B -Tested for antigenicity by Rosseels et al (2006) This, in turn, greatly impairs determination of livestock herd infectious disease status-critical for the entire livestock agronomy.…”