Diva technology: indispensable tool for the control of Johne’s disease

Jayaraman, Sujatha; Jain, Mukta; Dhama, Kuldeep; Singh, Shoor Vir; Sv, Singh; Datta, Manali; Jain, Neelam; Chaubey, Kundan Kumar; Chaubey, Kundan Kumar; Gupta, Saurabh; Aseri, Gajender Kumar; Khare, Neeraj; Yadav, Parul; Ak, Bhatia; Bhatia, Ashok Kumar; Js, Sohal; Sohal, Jagdip Singh

doi:10.18006/2015.4(1).16.25

Cited by 4 publications

(4 citation statements)

References 53 publications

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“…Due to the subclinical and chronic nature of disease, prolonged course of infection, difficulty in early diagnosis, control of paratuberculosis has become a problematic task (Jayaraman et al, 2016). Vaccination is the only possible way to control the disease by reducing the morbidity and mortality which in turn will reduce the economic losses associated with the disease (Dhand et al, 2013;Singh et al, 2013;Sohal et al, 2015).…”

Section: Histopathological Resultsmentioning

confidence: 99%

Molecular and histopathological evaluation of the efficacy of Saponifiedand Freund’s incomplete adjuvanted paratuberculosiskilled vaccine in murine model

Begum

Lingaraju

2017

IJAR

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Johne's disease (JD) is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Vaccination is regarded as the only cost effective method for controlling JD. Experimental trial for 11 months was carried out by dividing mice into four different groups. Group I-saponified MAP killed vaccine; Group II-Freund's incomplete adjuvant (FIA) MAP killed vaccine; Group III-saponin adjuvant control; Group IV-FIA control. Mice of all the groups were challenged with 10 8 colony forming unit live MAP organisms on 40 th day after vaccination. A total of 188 samples (52 faecal, 76 sera and 60 tissue samples) were collected from different groups of mice at different interval for monitoring MAP load by Ziehl Neelsen's staining and nested PCR along with tissue histopathology. Both the vaccinated groups (Group I and II) showed encouraging results in comparison to adjuvant control groups. There was reduction in faecal shedding (p<0.01), tissue load of MAP and less granuloma formation in both the vaccinated groups, however mice vaccinated with saponified MAP antigen gave better result in comparison to FIA vaccinated mice. Results of this study suggested that, saponified MAP killed vaccine in particular might reduce the overall burden of JD and show promising result in future.

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Section: Histopathological Resultsmentioning

confidence: 99%

Molecular and histopathological evaluation of the efficacy of Saponifiedand Freund’s incomplete adjuvanted paratuberculosiskilled vaccine in murine model

Begum

Lingaraju

2017

IJAR

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“…Development of MAP Diagnostics and Vaccine will require a cocktail of antigens due to the range of heterogeneous MHC molecules of out-bred animals [8] and to develope DIVA (Differentiation of Infected from Vaccinated Animals) strategy [9,10]. Since IS900 is unique to M. avium subsp.…”

Section: Discussionmentioning

confidence: 99%

IMMUNOREACTIVE RECOMBINANT NITRATE REDUCTASE EXPRESSED BY NIR A GENE OF Mycobacterium avium sub sp paratuberculosis IS900 ELEMENT IN E.coli

Padmavathy¹,

Vijayarani²,

Kumanan³

et al. 2018

BIOP

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“…Suitable dosages defined in terms of colony forming units (cfu) per dose have to be determined. Live vaccines require a bit of nutrient to support the life and side by side there is need to keep the cfu at constant level therefore live vaccines need a suitable excipient when the vaccine is not a freeze dried one and when it is freeze dried it need a suitable freeze drying and re-suspension medium to be chosen after experimentation [7].…”

Section: Diva Designing and Development Strategiesmentioning

confidence: 99%

Review on Discriminatory Tests of Immune Reaction due to Infection and Vaccination (DIVA Test)

2023

austinjvetscianimhusb

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In concern for animal diseases, the goal of vaccination is to prevent or reduce clinical diseases associated with the infectious agent, but it can also be used as a means of managing or eradicating a disease from a particular region. Therefore it is essential to differentiate immune responses due to vaccination compared to natural infection. It was to satisfy this requirement that the term Differentiation of Infected from Vaccinated Animals (DIVA) was coined in 1999 by Jan T van Oirschot. DIVA principle is based on a DIVA vaccine producing an antibody response that is different from the antibody response produced by the wild-type virus. The DIVA approach has been applied and proposed successfully to various diseases. Although considerable progress has been made to evaluate which different DIVA strategies are most likely to be applicable in the field, considerably more work needs to be done. If properly applied, DIVA vaccination strategies promise to provide new tools for the control and eradication of diseases.

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Diva technology: indispensable tool for the control of Johne’s disease

Cited by 4 publications

References 53 publications

Molecular and histopathological evaluation of the efficacy of Saponifiedand Freund’s incomplete adjuvanted paratuberculosiskilled vaccine in murine model

Molecular and histopathological evaluation of the efficacy of Saponifiedand Freund’s incomplete adjuvanted paratuberculosiskilled vaccine in murine model

IMMUNOREACTIVE RECOMBINANT NITRATE REDUCTASE EXPRESSED BY NIR A GENE OF Mycobacterium avium sub sp paratuberculosis IS900 ELEMENT IN E.coli

Review on Discriminatory Tests of Immune Reaction due to Infection and Vaccination (DIVA Test)

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