Scutellaria barbata D. Don induces c-fos gene expression in human uterine leiomyomal cells by activating beta2-adrenergic receptors

Abstract

Scutellaria barbata D. Don (Lamiaceae; SB) inhibited the growth of uterine leiomyomal (LM) cells with unknown actions. The expression patterns of beta-adrenergic receptors (beta-ARs) in human uterine LM cells and functional coupling to gene expression have also been investigated. Northern blot analysis showed that beta-AR subtypes are expressed at different levels in the uterine LM cells and myometrial smooth muscle cells (SMCs). beta1-AR expression was to be found approximately at the same level in the two cell types. beta2-ARs were expressed at higher levels in uterine LM cells than that in myometrial SMCs. beta3-AR expression was not found in both the cells. c-fos gene expression was induced by SB in uterine LM cells via increases in adenosine-3',5', cyclic monophosphate (cAMP), which in turn activated the cAMP/protein kinase A (PKA) pathway. The PKA inhibitor, H89, inhibited c-fos gene expression induced by SB. It seems that the mechanism of proto-oncogenes c-fos different leiomyoma from other myometrial cancer. Further studies are necessary to elucidate whether c-fos induction by SB in uterine LM cells influences a regression of leiomyoma or induces other differentiation.

Characterization and quantification of the chemical compositions of Scutellariae Barbatae herba and differentiation from its substitute by combining UHPLC–PDA–QTOF–MS/MS with UHPLC–MS/MS

“…(Labiatae). It has been used as a treatment for boils, swollen poison, sore throat and venomous snake bites for thousands of years in China [1]. Modern pharmacological studies have showed that S. barbata (SB) has a wide variety of medical applications due to its antioxidant, anticancer, as well as anti-angiogenic effects, etc.…”

Chemoprevention of Scutellaria bardata on human cancer cells and tumorigenesis in skin cancer

“…The responses of both normal uterine myometrial SMC and leiomyomal SMCs to SB treatment were analysed. Cell cycle analysis using flow cytometry and Western blotting using antibodies against α-SMA, calponin h1 and the G1 cell-cycle-related gene products, cyclin E, cyclindependent kinase 2 (cdk2) and the cdk inhibitor, p27, clearly showed its effectiveness on anti-growth activity of leiomyomal SMCs (Lee et al, 2004a;Lee et al, 2004b). Recently, it was demonstrated that SB inhibits cell proliferation dependent on an apoptotic response.…”

Euonymus alatus (Thunb.) Sieb induces apoptosis via mitochondrial pathway as prooxidant in human uterine leiomyomal smooth muscle cells

“…Human ULSMC and normal human myometrial smooth muscle cells were used in this study, as isolated and described previously (8,18,19) . Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized venous blood of healthy volunteers by Ficoll‐Paque density gradient centrifugation (20) .…”