Characterization and quantification of the chemical compositions of Scutellariae Barbatae herba and differentiation from its substitute by combining UHPLC–PDA–QTOF–MS/MS with UHPLC–MS/MS

Zhang, Zhifeng; He, Lili; Lu, Luyang; Liu, Yuan; Dong, Gengting; Jin, Miao; Luo, Pei

doi:10.1016/j.jpba.2015.02.025

Cited by 34 publications

(6 citation statements)

References 15 publications

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“…Therefore, compound 6 was tentatively identified to be quercetin 3-O-β-D-glucuronide, which was consistent with previous literature 17. Compounds 7, 8, and 12 were assigned as isoquercetin, kaempferol-3-rutinoside, and baicalin, respectively, based on the same flavone fragment ion (179), matching of molecular formulas, comparison of UV data, and high-energy CID fragmentation information reported in the literature 18. T A B L E 23.3.2 | Identification of phenolic acid and its glucuronideTen phenolic acid compounds (compounds 1, 2, 3, 4, 9, 10, 11, and 13) and two phenolic acid glucuronides were identified or tentatively characterised in…”

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confidence: 85%

Chemotaxonomy of Aster species from the Qinghai‐Tibetan Plateau based on metabolomics

et al. 2021

Phytochemical Analysis

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Introduction:The genus Aster plants have been widely used for thousands of years in the Qinghai-Tibetan Plateau for the clearing of heat, detoxification, and the treatment of seasonal pandemic diseases. Although the presence of several flavonoid compounds in Aster has been reported by previous studies, the diversity of secondary metabolites within and among species is relatively unknown. Objective:The metabolite profile of one Aster species was systematically compared with those of other taxa to find potential chemotaxonomic markers, delimit species, and assess chemodiversity.Method: Samples of the above-ground parts of 11 Aster species were collected and their metabolites were analysed by ultra-high performance liquid chromatography with photodiode array detection and quadrupole time-of-flight tandem mass spectrometry. Unsupervised principal component analysis, supervised orthogonal partial least-squares discriminant analysis, heatmap analysis, and hierarchical cluster analysis were employed to analyse 95 representative samples from 11 Aster species and determine species-specific chemical markers based on a metabolomics database.Results: Six phenolic acids and flavonoids were detected and quantified in all Aster species, suggesting that these compounds may be common constituents in the Aster genus. Metabolite analysis showed terpenoid compounds to be potential chemical markers for interspecies differentiation. Ent-kaurane-type diterpenoid glycosides were the main class of compounds in all Aster species except for A. farreri, which mainly contained oleanane-type pentacyclic triterpenoids. Diterpenoid glycosides were low-content chemotaxonomic markers and were detected for the first time in Aster species from the Qinghai-Tibetan Plateau. Conclusion:Chemotaxonomy and metabolomics were used to support the phylogenic relationships of the Aster genus.

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confidence: 85%

Chemotaxonomy of Aster species from the Qinghai‐Tibetan Plateau based on metabolomics

et al. 2021

Phytochemical Analysis

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“…Sixteen of them were definitely identified by comparison of their retention time, precise molecular mass and MS/MS fragment patterns with the reference standards. The identification of another 52 components was based on software and the literature data (Huang, Wang, Jin, Shen, & Weng, ; Liang et al, ; Liu, Zhang, Gao, Jiang, & Chen, ; Ma, Gong, Zhou, Zhao, & Li, ; Qian et al, ; Xie, Wang, Zhang, Bai, & Yang, ; Zhang et al, ; Zhao, Long, Dai, Bi, & Chen, ). The representative total ion chromatogram of the ZSF extract is displayed in Fig.…”

Section: Investigations and Resultsmentioning

confidence: 99%

Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis

Zheng

Zhang

Geng

et al. 2017

Biomedical Chromatography

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An ultra-high-performance liquid chromatography mass spectrometry method was established to detect and identify the chemical constituents of Zi Shen Formula (ZSF) and its metabolites in serum, urine and feces, after oral administration to rats. A total of 68 compounds were characterized in ZSF extracts. In vivo, 38 prototype components and 32 metabolites of ZSF were tentatively identified in rat serum, urine and feces. Seven metabolic pathways including demethylation, hydroxylation, oxidation, sulfation, glucuronidation, methylation and de-caffeoyl were proposed to be involved in the generation of these metabolites. It was found that glucuronidation, methylation and demethylation were the major metabolic processes of alkaloids, while demethylation, methylation, sulfation and de-caffeoyl were the major metabolic pathways of phenylethanoid glycosides. The main metabolic pathways of steroidal saponins were oxidation and isotype reactions. These findings are significant for our understanding of the metabolism of ZSF. The proposed metabolic pathways of bioactive components might be crucial for further studies of the mechanisms of action and pharmacokinetic evaluations of ZSF.

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“… Compound Precursor Ion ( m / z ) Daughter Ion ( m / z ) Ref. 21 289 248, 227, 217, 203, 188, 164, 151, 125, 123 [ 45 ] 22 269 227, 195, 183, 151, 121, 117, 107 [ 46 ] 23 301 273, 229, 178, 151 [ 37 ] 24 463 300, 301, 303 [ 37 ] 25 431 269, 225, 197, 183, 169, 151, 121, 117 [ 46 ] 26 447 285, 254 [ 47 ] 27 537 [ 48 ], standard 28 537 - [ 48 ], standard 29 385 …”

Section: Figure A1mentioning

confidence: 99%

Polyphenolic Profile and Targeted Bioactivity of Methanolic Extracts from Mediterranean Ethnomedicinal Plants on Human Cancer Cell Lines

et al. 2016

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Abstract:The methanol extracts of the aerial part of four ethnomedicinal plants of Mediterranean region, two non-seed vascular plants, Equisetum hyemale L. and Phyllitis scolopendrium (L.) Newman, and two Spermatophyta, Juniperus communis L. (J. communis) and Cotinus coggygria Scop. (C. coggygria), were screened against four human cells lines (A549, MCF7, TK6 and U937). Only the extracts of J. communis and C. coggygria showed marked cytotoxic effects, affecting both cell morphology and growth. A dose-dependent effect of these two extracts was also observed on the cell cycle distribution. Incubation of all the cell lines in a medium containing J. communis extract determined a remarkable accumulation of cells in the G2/M phase, whereas the C. coggygria extract induced a significant increase in the percentage of G1 cells. The novelty of our findings stands on the observation that the two extracts, consistently, elicited coherent effects on the cell cycle in four cell lines, independently from their phenotype, as two of them have epithelial origin and grow adherent and two are lymphoblastoid and grow in suspension. Even the expression profiles of several proteins regulating cell cycle progression and cell death were affected by both extracts. LC-MS investigation of methanol extract of C. coggygria led to the identification of twelve flavonoids (compounds 1-11, 19) and eight polyphenols derivatives (12)(13)(14)(15)(16)(17)(18)20), while in J. communis extract, eight flavonoids (21-28), a α-ionone glycoside (29) and a lignin (30) were found. Although many of these compounds have interesting individual biological activities, their natural blends seem to exert specific effects on the proliferation of cell lines either growing adherent or in suspension, suggesting potential use in fighting cancer.

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Characterization and quantification of the chemical compositions of Scutellariae Barbatae herba and differentiation from its substitute by combining UHPLC–PDA–QTOF–MS/MS with UHPLC–MS/MS

Cited by 34 publications

References 15 publications

Chemotaxonomy of Aster species from the Qinghai‐Tibetan Plateau based on metabolomics

Chemotaxonomy of Aster species from the Qinghai‐Tibetan Plateau based on metabolomics

Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis

Polyphenolic Profile and Targeted Bioactivity of Methanolic Extracts from Mediterranean Ethnomedicinal Plants on Human Cancer Cell Lines

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