Screening the organs for early detection of white spot syndrome virus in Penaeus indicus by histopathology and PCR techniques

Yoganandhan, K.; Sathish, S.; Murugan, Vadivel; Narayanan, R. B.; Hameed, A.S. Sahul

doi:10.1016/s0044-8486(02)00123-0

Cited by 44 publications

(42 citation statements)

References 24 publications

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“…The cellular pathology of WSSV infected P. monodon observed in the present study is in agreement with the observation of Nash and Akarajamorn (1995), Chang et al (1996) and Yoganandhan et al (2003). At the very early asymptomatic and subclinical stages of infection, up to 18 h p.i., only the PCR gave positive results, indicating the sensitivity and advantage of PCR over histological methods.…”

Section: Discussionsupporting

confidence: 93%

“…Histopathological evidence has shown that this virus replicates in tissues of ectodermal and mesodermal origin only (Chou et al 1995;Lightner 1996;Karunasagar et al 1997;Mohan et al 1997;Rajendran et al 1999, Yoganandhan et al 2003Vijayan et al 2003). Evidence of histopathological manifestations in the target tissues is one of the criteria used in the diagnosis of WSSV infection (Lightner 1996;Wang et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

et al. 2005

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

et al. 2005

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“…Since its advent in 1992, WSV has greatly affected the shrimp farming industry (Mohan et al, 1997). This pathogen has tropism for organs of mesodermal and ectodermal origin (Wongteerasupaya et al,1995(Wongteerasupaya et al, , 1996Chang et al, 1996;Flegel et al, 1997;Rajendran et al, 1999;Wang et al, 1999;Vijayan et al, 2003;Yoganandhan et al, 2003). Evidence of histopathological manifestations in the target tissues is one of the criteria used in the diagnosis of WSV infection (Lightner, 1996;Wang et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…At least 18 cultured and/or wild penaeid shrimp (Park et al, 1998), eight caridean species (Pramod-Kiran et al, 2002), seven species of lobster (Rajendran et al, 1999), seven species of crayfish (Edgerton, 2004), 38 crab species (Yoganandhan et al, 2003) six non-decapod crustacean species (Hossain et al, 2001), members of the phyla Chaetognata and Rotifera (Yan et al, 2004), polychaete worms (Supak et al, 2005) and some aquatic insect larva (Ramírez-Douriet et al, 2005) have been found susceptible to the virus. Histopathological observations during WSV infection in brief is available regarding shrimp species such as Penaes monodon (Durand et al, 1997;Wang et al, 1999Wang et al, , 2000Mishra and Shekhar, 2005) Litopenaeus vannamei and Marsupenaeus japonicus (Lu et al, 1997;Lightner et al, 1998;Escobedo-Bonilla et al, 2007;Perez et al, 2005;Pantoja and Lightner, 2003), species of Crabs (Kanchanaphum et al, 1998;Kou et al, 1998) and lobster (Rajendran et al, 1999;Wang et al, 1998;Jiravanichpaisal et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis

Manjusha

Varghese

Philip

et al. 2009

Journal of Invertebrate Pathology

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a b s t r a c tTo demonstrate pathological changes due to white spot virus infection in Fenneropenaeus indicus, a batch of hatchery bred quarantined animals was experimentally infected with the virus. Organs such as gills, foregut, mid-gut, hindgut, nerve, eye, heart, ovary and integument were examined by light and electron microscopy. Histopathological analyses revealed changes hitherto not reported in F. indicus such as lesions to the internal folding of gut resulted in syncytial mass sloughed off into lumen, thickening of hepatopancreatic connective tissue with vacuolization of tubules and necrosis of rectal pads in hindgut. Virus replication was seen in the crystalline tract region of the compound eye and eosinophilic granules infiltrated from its base. In the gill arch, dilation and disintegration of median blood vessel was observed. In the nervous tissues, encapsulation and subsequent atrophy of hypertrophied nuclei of the neurosecretory cells were found. Transmission electron microscopy showed viral replication and morphogenesis in cells of infected tissue. De novo formed vesicles covered the capsid forming a bilayered envelop opened at one end inside the virogenic stroma. Circular vesicles containing nuclear material was found fused with the envelop. Subsequent thickening of the envelop resulted in the fully formed virus. In this study, a correlation was observed between the stages of viral multiplication and the corresponding pathological changes in the cells during the WSV infection. Accordingly, gill and foregut tissues were found highly infected during the onset of clinical signs itself, and are proposed to be used as the tissues for routine disease diagnosis.

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“…The PLs were tested beforehand for White Spot Syndrome Virus (WSSV) by a Polymerase Chain Reaction (PCR) test (Durand & Lightner, 2002;Yoganandhan et al, 2003). The shrimp PLs were transported from the hatchery for five hours using a closed system at a density of 1,000 individuals/L.…”

Section: Fcr = F Bmentioning

confidence: 99%

HIGH DENSITY JUVENILE SHRIMP (Penaeus monodon) PRODUCTION WITH DIFFERENT DURATIONS OF REARING IN ACID SULFATE SOIL-AFFECTED POND

Mustafa

Sammut

2010

Indonesian Aquaculture Journal

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An experiment was conducted to evaluate the effectiveness of producing juvenile shrimp in a reservoir pond constructed in acid sulphate soils (ASS). Many ponds constructed in ASS pose a significant economic risk due to high mortality and poor growth. In Indonesia, abandonment of ponds in ASS is widespread and alternative farming practices, such as production of juvenile shrimp, offer farms opportunities to lower their production risks. The experiment involved rearing juvenile shrimp at a high stocking density (3,000 individuals/m 2 ) using hapas in a formerly unproductive pond located in Luwu Regency, South Sulawesi, Indonesia. The treatments tested included rearing periods of three and six weeks with four replications. A survival of 83.62% was achieved after rearing for three weeks compared to 45.44% after six weeks. Although there was a sixfold increase in the mean weight of shrimp from three to six weeks of culture, size variation after six weeks was large due to the high density of shrimp. The experiment showed that old acid sulphate soil-affected ponds, in which the bottom soil has not been excavated any further, can be used for production of juvenile shrimp in hapas. Rearing over three weeks is recommended to obtain high survival and reduce size variation.

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Screening the organs for early detection of white spot syndrome virus in Penaeus indicus by histopathology and PCR techniques

Cited by 44 publications

References 24 publications

White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis

HIGH DENSITY JUVENILE SHRIMP (Penaeus monodon) PRODUCTION WITH DIFFERENT DURATIONS OF REARING IN ACID SULFATE SOIL-AFFECTED POND

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