White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

Rajendran, K. V.; Vijayan, K.K.; Santiago, T.C.; Rajan, J.J.S.

doi:10.1007/s10499-004-6549-5

Cited by 10 publications

(6 citation statements)

References 17 publications

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“…; Rajendran et al . ). In the present study, we used shrimp orally infected with WSSV, which is a natural form of infection and thus closer to field conditions.…”

Section: Discussionmentioning

confidence: 97%

“…As there are no shrimp cell lines suitable for the evaluation of detection methods (Prior et al 2003), detection of the virus following experimental infection of shrimp is the most appropriate strategy at present. Furthermore, there are several methods for artificially infecting shrimp with WSSV, such as immersion, injection and oral feeding (Chou et al 1998;Tan et al 2001;Yoganandhan et al 2003;Rajendran et al 2005). In the present study, we used shrimp orally infected with WSSV, which is a natural form of infection and thus closer to field conditions.…”

Section: A B C Dmentioning

confidence: 99%

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Development of a monoclonal antibody‐based flow‐through immunoassay (FTA) for detection of white spot syndrome virus (WSSV) in black tiger shrimp Penaeus monodon

Patil

Shankar

Kumar

et al. 2013

Journal of Fish Diseases

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A flow-through immunoassay (FTA), an improved version of immunodot, was developed using a nitrocellulose membrane baked onto adsorbent pads enclosed in a plastic cassette to detect white spot syndrome virus (WSSV) in shrimp. Sharp purple dots developed with WSSV against the white background of the nitrocellulose membrane. The detection limits of WSSV by the FTA and immunodot were 0.312 and 1.2 μg mL(-1) crude WSSV protein, respectively. The FTA could be completed in 8-10 min compared with 90 min for immunodot. The FTA was 100 times more sensitive than 1-step polymerase chain reaction (PCR) and in between that of the 1- and 2-step PCR protocol recommended by the Office of International Epizootics (OIE). In experimental, orally infected shrimp post-larvae, WSSV was first detected 14, 16 and 18 h post-infection (hpi) by FTA, immunodot and one-step PCR, respectively. The FTA detected WSSV 2 and 4 h earlier than immunodot and one-step PCR, respectively. The FTA was more sensitive (25/27) than one-step PCR (23/27) and immunodot (23/27) for the detection of WSSV from white spot disease outbreak ponds. The reagent components of the FTA were stable giving expected results for 6 m at 4-8 °C. The FTA is available as a rapid test kit called 'RapiDot' for the early detection of WSSV under field conditions.

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“…; Rajendran et al . ). In the present study, we used shrimp orally infected with WSSV, which is a natural form of infection and thus closer to field conditions.…”

Section: Discussionmentioning

confidence: 97%

Section: A B C Dmentioning

confidence: 99%

Development of a monoclonal antibody‐based flow‐through immunoassay (FTA) for detection of white spot syndrome virus (WSSV) in black tiger shrimp Penaeus monodon

Patil

Shankar

Kumar

et al. 2013

Journal of Fish Diseases

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show abstract

“…Previous studies showed that pathological changes were more obvious and typical at the moribund stage than at early infection phases in WSSV-infected shrimp (Yoganandhan et al, 2003;Rajendran et al, 2005). Therefore pathological changes could easily be observed at the moribund stage in infected shrimp.…”

Section: Discussionmentioning

confidence: 86%

“…This virus has been found to be highly pathogenic not only to penaeid shrimp, but also to other invertebrate aquatic organisms such as crabs and copepods Chakraborty et al, 2002). Previous studies were carried out on the pathogenicity and development of diagnostic methods to detect WSSV Lo et al, 1996;Rajendran et al, 2005), but few researches were carried out on the morphological hematological pathology in penaeid shrimp infected by WSSV (Wang et al, 2002;Jiravanichpaisal et al, 2006). Concerning Fenneropenaeus chinensis, the study on hematological pathology of WSS was not performed despite the fact that the shrimp is an economically important seafood and its culture has become popular recently in Asia (Zhan et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

Shu

Zhan

Xing

et al. 2008

J. Ocean Univ. China

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The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.

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“…A typical outbreak starts in the first day or two after the introduction of virus or virus-infected shrimp, followed by a mass mortality of 70-100% in the next five to seven days [43]. This is primarily due to horizontal transmission of the virus [44]. Exploring the compound efficiency against horizontal WSSV transmission is important to B-F) Aquacultural water or fresh C2 and C7 medium were exchanged at 0, 1, 2, 3, and 4 days, respectively.…”

Section: The Effect Of C2 and C7 On The Horizontal Transmission Of Wssvmentioning

confidence: 99%

Small Molecule Inhibitors of White Spot Syndrome Virus: Promise in Shrimp Seedling Culture

Liu

Shan

Zhou

et al. 2021

IJMS

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Rapid production of prawn (Litopenaeus vannamei) under artificial pressure can result in a series of obvious challenges and is vulnerable to serious losses related to aquatic environmental issues and the unrestrained outbreak of white spot syndrome (WSS). However, to date, there are no therapeutic strategies to contain the spread of the virus. Here, we synthesized 27 coumarin deriv-atives and evaluated their anti-white spot syndrome virus (WSSV) activity in L. vannamei larvae. We demonstrated that electron-withdrawing and electron-giving substituent groups play an im-portant role in reducing toxicity and WSSV replication, respectively. Two coumarin C2 (2-amino-5-oxo-4-(p-tolyl)-4H,5H-pyrano[3,2-c]chromene-3-carbonitrile) and C7 (2-amino-4-(4-chlorophenyl)-5-oxo-4H,5H-pyrano[3,2-c]chromene-3-carbonitrile) were regarded as the most promising anti-WSSV compounds with maximum antiviral response <5% and median effective concentration <10 mg/L. The mortality of WSSV-infected larvae decreased by more than 60% after exposure to C2 and C7. With continuous immersion of C2 and C7 exchange, the mortality further decreased to 40% at 120 h.Additionally, C2 and C7 are the relatively stable in aquacultural water, making these agents

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White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

Abstract: Abstract. White spot syndrome virus (WSSV) infection was induced in tiger shrimp, Penaeus monodon, under laboratory conditions, and histopathological changes in subcuticular epithelial cells of the eye stalk and pleopod were studied sequentially at different time post-challenge.

Cited by 10 publications

References 17 publications

Development of a monoclonal antibody‐based flow‐through immunoassay (FTA) for detection of white spot syndrome virus (WSSV) in black tiger shrimp Penaeus monodon

Development of a monoclonal antibody‐based flow‐through immunoassay (FTA) for detection of white spot syndrome virus (WSSV) in black tiger shrimp Penaeus monodon

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

Small Molecule Inhibitors of White Spot Syndrome Virus: Promise in Shrimp Seedling Culture

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