Screening of protease producing bacteria from tannery wastes of leather processing industries at Hazaribag, Bangladesh

Rahman, Saimoon; Islam, Rayhan; Mondol, Omit Kumer; Rahman, Mohammad Shahedur; Sabrin, Farah; Zohora, Umme Salma

doi:10.3329/jujbs.v7i1.37970

Cited by 10 publications

(6 citation statements)

References 17 publications

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“…SMA is composed of skim milk powder (28 g/L), casein enzymichydrolysate (5 g/L), Yeast extract (2.5 g/L), dextrose (1 g/L), agar 15 (g /L) with final pH 7. The plates are inoculated with a population of microorganisms to test for the ability to digest casein hydrolysis (Rahman et al, 2018).…”

Section: Amylolytic Activity Assaymentioning

confidence: 99%

Investigation on a Bangladeshi isolate Bacillus aryabhattai for promising biotechnological applications

Rahman

Banu

Moni³

et al. 2018

Jahangirnagar Univ. J. Biol. Sci.

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A new isolate was investigated from soil sample collected from Shahrasti upazilla of Chandpur district of Bangladesh. Based on the physico-chemical studies the strain was identified as gram positive Bacilli. Moleculer characterization of the strain was identified as Bacillus aryabhattai which is the first report in Bangladesh. The strain can survive in extreme conditions of salt, temperature and pH. This strain was further characterized and screened for the ability to produce useful enzymes. The optimum temperature for growth and production of these enzymes was within the temperature range 35oC to 40oC. The pH was found to be 7 for its growth and production of different enzymes when investigated over 48 h of incubation. The isolate produced various extracellular enzymes such as α-amylases, cellulases, β-glucosidases, lipases and proteases. The findings of this study provide useful information of the new strain that has potential biotechnological applications. Jahangirnagar University J. Biol. Sci. 7(2): 33-45, 2018 (December)

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Section: Amylolytic Activity Assaymentioning

confidence: 99%

Investigation on a Bangladeshi isolate Bacillus aryabhattai for promising biotechnological applications

Rahman

Banu

Moni³

et al. 2018

Jahangirnagar Univ. J. Biol. Sci.

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“…Similarly, the optimal conditions of extracellular protease-producing bacteria were concerned with B. velezensis (Khalid et al, 2021). The B. velezensis bacteria were found 19.54 U/mg of enzyme activity, which is similar to B. pumilus (63.58 U/ml; Gomaa, 2013) and B. subtilis (27.19-60.50 U/ml;Rodarte et al, 2011;Rahman et al, 2018). As a result, proteolytic enzymes generated from Bacillus proteases have been shown to produce intriguing outcomes.…”

Section: Discussionmentioning

confidence: 91%

“…The 58 isolates of protease-producing bacteria were carried out utilizing the agar well diffusion technique ( Rahman et al, 2018 ). Bacteria that had been stored were grown in NB media and incubated at 37°C for 24 h. The bacteria (10 μl) were then introduced to skim milk agar (SMA; Oxoid, England) media and cultured at 37°C for 72 h to examine the protease-producing zone on the media.…”

Section: Methodsmentioning

confidence: 99%

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

et al. 2022

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Earthworms have long been used as traditional medicine. The purposes of this research were to create bioactive peptides from the unique Amynthas arenulus earthworm (PAAEs) and test their potentials on liver cancer bioprophylactic activity, antioxidant, oxidative stress protection, and immune cell activation. This earthworm had a high protein content ratio, at 55.39%. Besides, PM 35 is one out of 58 bacteria isolated from the earthworm carcasses that exhibited the highest protease and yield protein production which was chosen as the protease-producing bacteria to hydrolyze the protein. The genera were identified by 16S rRNA and 16S–23S rRNA comparison and confirmed as Bacillus velezensis PM 35. The response surface methodology was applied to optimize these hydrolysis parameters, i.e., the enzyme/substrate (E/S) concentration ratio [1%–3% (v/v)] and time (1–3 h) of the hydrolyzing earthworm’s proteins. The optimal hydrolyzing conditions were 3% (v/v) of E/S concentration ratio and 3 h of hydrolysis time, which found protein-hydrolysate yield (24.62%) and degree of hydrolysis (85.45%) as the highest. After being challenged in the gastrointestinal tract-resistant model, these PAAEs (MW <3 and 3–5 kDa) induced liver cancer cell (HepG2) death via apoptotic action modes (cell morphological change and DNA fragmentation). The PAAEs (MW <3 kDa) exhibited significant antioxidant activity via DPPH, ABTS, and FRAP with IC50 values of 0.94, 0.44, and 6.34 mg/ml, respectively. The PAAEs (MW < 3 kDa) were non-cytotoxic and protected the mouse fibroblast cells (L929) against oxidative stress. These PAAEs (MW < 3 kDa, 0.2 mg/ml) stimulated the B lymphocytes (122.3%), and T lymphocytes (126.7%) proliferation. This research suggests that PAAEs can be used in a variety of applications, especially in the food and pharmaceutical industries.

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“…The isolates were further screened for the heavy metals in Luria-Bertani (LB) agar media supplemented with 100 ppm (~100 mg/L) of PbNO3, CuSO4, K2Cr2O7, CdCl2.H2O and NiCl2.6H2O. The inhibition in growth of bacteria indicates positive results [23,24].…”

Section: Isolation Of Heavy Metal Resistant Bacteriamentioning

confidence: 99%

Isolation and Characterization of Heavy Metal-Tolerant Bacterial Isolates from Industrial Effluents in Uttar Pradesh, India

Gangwar,

Tripathi,

Verma

2024

IJECC

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Industrial activities over the past century have significantly increased human exposure to pollutants such as heavy metals. Industrial emissions of heavy metals, which are carcinogenic, mutagenic and toxic, contaminate natural water supplies and the agricultural environment. Due to the high concentration of heavy metals in industrial effluents, the bacteria present there naturally develop resistance to heavy metals. The aim of this study is to isolate and characterize bacteria resistant to heavy metal, lead (Pb), copper (Cu), chromium (Cr), cadmium (Cd), and nickel (Ni) from sites contaminated by industrial effluents in Uttar Pradesh, India. A total of 58 bacterial isolates were isolated from 9 samples according to their various morphological parameters and 32 isolates were found positive for all heavy metals, which revealed that samples contained metal-resistant bacterial diversity. Two isolates were identified up to species level based on their physiological, biochemical and molecular characterization as Comamonas testosteroni (S4C1) and Bacillus cereus (S5C3). Both isolates are highly resistant to lead (Pb), copper (Cu), chromium (Cr), cadmium (Cd), and nickel (Ni) and they show different MICs against the above heavy metals at different levels. A growth experiment showed that the presence of heavy metals concentration had no discernible impact on the growth rate among the isolates. Gel analysis showed interesting patterns of protein expression were observed in the presence of various heavy metals. MALDI-TOF analysis found that specific proteins (S layer protein, F0F1ATP synthase subunit b, Flagellin, 50S ribosomal protein L4, Molecular chaperone) were overexpressed in the presence of heavy metals. As a result, identifying the heavy metal resistance bacteria and their proteins study could be useful as a preliminary investigation for the development of prospective bioremediation agents of potentially hazardous waste treatment technology.

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Screening of protease producing bacteria from tannery wastes of leather processing industries at Hazaribag, Bangladesh

Cited by 10 publications

References 17 publications

Investigation on a Bangladeshi isolate Bacillus aryabhattai for promising biotechnological applications

Investigation on a Bangladeshi isolate Bacillus aryabhattai for promising biotechnological applications

Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation

Isolation and Characterization of Heavy Metal-Tolerant Bacterial Isolates from Industrial Effluents in Uttar Pradesh, India

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