Investigation on a Bangladeshi isolate Bacillus aryabhattai for promising biotechnological applications

Abstract

A new isolate was investigated from soil sample collected from Shahrasti upazilla of Chandpur district of Bangladesh. Based on the physico-chemical studies the strain was identified as gram positive Bacilli. Moleculer characterization of the strain was identified as Bacillus aryabhattai which is the first report in Bangladesh. The strain can survive in extreme conditions of salt, temperature and pH. This strain was further characterized and screened for the ability to produce useful enzymes. The optimum temperature for growth and production of these enzymes was within the temperature range 35oC to 40oC. The pH was found to be 7 for its growth and production of different enzymes when investigated over 48 h of incubation. The isolate produced various extracellular enzymes such as α-amylases, cellulases, β-glucosidases, lipases and proteases. The findings of this study provide useful information of the new strain that has potential biotechnological applications.
 Jahangirnagar University J. Biol. Sci. 7(2): 33-45, 2018 (December)

Study of an arsenic metabolizing bacteria from arsenic contaminated soil of Chandpur district, Bangladesh

“…Among the different isolates, I-25 is found to be a novel arsenic metabolizing strain that was previously identified as B. aryabhatti according to the morphological, biochemical and molecular characteristics as reported earlier. In addition to that the ability of this strain to produce α-amylases, cellulases, βglucosidases, lipases and proteases was reported before by our group (Rahman et al, 2018). In the present investigation, the bacterial species B. aryabhatti showed highly active arsenic metabolizing activity as it was able to change the color of starch iodine complex within 5min of incubation at dark condition.…”

“…After 16 h of incubation, stocks were prepared by dissolving the culture in equal volume with 40% glycerol. After single colony isolation, the morphological, biochemical and molecular characteristics of the isolated bacterial strains were also evaluated as previously described by Rahman et al (2018).…”

“…The present study reports B. aryabhatti with the ability to metabolize arsenic and reduce As (V) into As (III) for the first time in Bangladesh. This B. aryabhatti has been previously identified and reported by our group to produce various extracellular enzymes such as α-amylases, cellulases, β-glucosidases, lipases and proteases (Rahman et al, 2018). However, the ability of this bacterium for arsenic metabolizing activity was never reported and there had been no report about the presence of this bacterium in arsenic contaminated samples.…”

Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

“…Isolates were morphologically characterized by determining the production of diffuse pigments and the color of aerial and substrate mycelium on TSA medium and then classified into a color class [ 22 ]. Selected isolates were tested for enzyme activities (proteolytic, lipolytic, amylolytic and cellulolytic) using TSA medium supplemented with milk powder (1% w / v ), glycerol tributyrate (1% v / v ), Starch azure (0.65% w / v ) or carboxymethyl cellulose (2.6% w / v ) [ 23 , 24 , 25 ]. Growth at different temperatures (RT and 37 °C) was tested on TSA medium and growth at different pH (5 and 7) was tested on TSA medium with 50 mM TRIS (tris(hydroxymethyl)aminomethane) (0.6% v / v ), adjusted with NaOH solution.…”