Screening of potential bioremediation enzymes from hot spring bacteria using conventional plate assays and liquid chromatography - Tandem mass spectrometry (Lc-Ms/Ms)

Jardine, Jocelyn Leonie; Stoychev, Stoyan; Mavumengwana, Vuyo; Ubomba-Jaswa, Eunice

doi:10.1016/j.jenvman.2018.06.089

Cited by 36 publications

(10 citation statements)

References 67 publications

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“…In the fields of heavy metals bioremediation and biomonitoring many thermophilic oxidoreductases have also been characterized. For example, quinone oxidoreductase, chromate reductase, and superoxide dismutase from different Anoxibacillus species have been employed for Pb and Cr bioremoval [ 70 , 71 ]; moreover, the arsenate reductase from T. thermophilus HB27 ( Tt ArsC) has been exploited as the biological recognition element for the development of different arsenic biosensors [ 72 , 73 , 74 ].…”

Section: Heavy Metalsmentioning

confidence: 99%

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Gallo

Puopolo

Carbonaro

et al. 2021

IJERPH

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Extremophiles are microorganisms that populate habitats considered inhospitable from an anthropocentric point of view and are able to tolerate harsh conditions such as high temperatures, extreme pHs, high concentrations of salts, toxic organic substances, and/or heavy metals. These microorganisms have been broadly studied in the last 30 years and represent precious sources of biomolecules and bioprocesses for many biotechnological applications; in this context, scientific efforts have been focused on the employment of extremophilic microbes and their metabolic pathways to develop biomonitoring and bioremediation strategies to face environmental pollution, as well as to improve biorefineries for the conversion of biomasses into various chemical compounds. This review gives an overview on the peculiar metabolic features of certain extremophilic microorganisms, with a main focus on thermophiles, which make them attractive for biotechnological applications in the field of environmental remediation; moreover, it sheds light on updated genetic systems (also those based on the CRISPR-Cas tool), which expand the potentialities of these microorganisms to be genetically manipulated for various biotechnological purposes.

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Section: Heavy Metalsmentioning

confidence: 99%

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Gallo

Puopolo

Carbonaro

et al. 2021

IJERPH

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“…Derekova 16 was isolated and identified A. rupiensis, which could survive between 35-67°C, 5.5-8.5 pH, for the first time with ability to degrade xylan, starch and casein. Jardine 27 was also proposed A. rupiensis as amylase, protease and celllulase producer. Yanmis 58 isolated A. rupiensis from geothermal regions of Turkey and it was declared as amylase producer.…”

Section: Enzyme Production Capacities Of Thermophilesmentioning

confidence: 99%

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Albayrak¹,

Genç²,

Özkan³

et al. 2019

J Pure Appl Microbiol

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In this study, one hundred and thirty isolates were isolated from water and sludge samples taken from hot springs located in different regions of Turkey. Among them, eleven isolates were chosen according to conventional (morphological, physiological and biochemical tests) and molecular methods (rep-PCR and 16S rRNA sequencing). These bacteria were then tested for their capability to produce valuable enzymes. As a result; species belonging to Bacillus, Anoxybacillus, Aeribacillus, Enterococcus, Exiguobacterium and Paenibacillus were identified. Test strains were found to have optimum reproductive potential at pH 5.0-9.0 and 15-65°C, usually at a concentration of 1.0-10.0% (w/v) NaCl. In addition, all thermotolerant bacteria were Gram, endospore (except E. profundum), catalase and oxidase (except E. faecium and E. profundum) positive, and rod-shaped (except E. faecium). It was observed that all isolates had a 99% similarity percentage as a result of 16S rRNA sequence analysis. All of the isolates were capable of producing industrially important enzymes moreover, eight of them could produce at least two of these enzymes. Test strains had high potential of industrial enzyme production, and the enzymes from these thermo-tolerant isolates will be widely used in biotechnological processes.

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“…These thermophilic microorganisms are of great interest since they are able to produce thermostable enzymes and biomolecules such as biosurfactants, polysaccharides, and antibiotics, which are industrially important (Atalah et al 2019, Di Lorenzo et al 2014, Geraldi et al 2019a, Kambourova 2018, Mahajan and Balachandran 2017, Sahoo et al 2020, Teta et al 2017. These microorganisms can also be utilized in bioremediation processes such as the biodegradation of hydrocarbons and removal of heavy metals, and also biofuel production (Castro et al 2019, Di Donato et al 2019, Ebaid et al 2019, Geraldi et al 2019b, Jardine et al 2018, Orellana et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene

et al. 2021

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Abstract. Geraldi A, Tay CC, Ni’matuzahroh, Fatimah, Hanafi WNW. 2021. Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene. Biodiversitas 22: 4060-4066. This study is the first attempt at using the Next Generation Sequencing (NGS) method with 16S rRNA to understand the bacterial community structure in an Indonesian hot spring. This study aims to unravel the bacterial diversity of the Cangar Hot Spring as one of the most explored natural hot springs in East Java, Indonesia. We found Proteobacteria and Bacteroidetes as the two most abundant phyla. We discovered the first occurrence of genera Cloacibacterium and Methylobacillus in the hot spring ecosystem, which was the most dominant genera at Cangar Hot Spring. We also found several potential bacteria for bioindustry and bioremediation, such as Acinetobacter junii and Pseudomonas alcaligenes. Besides that, we also observed opportunistic pathogens from genera Comamonas and Vogesella. This study result will provide valuable information for further bioprospecting of bacteria with commercial potential and the development of health and safety measures in the Cangar Hot Spring, among others. Hopefully, this report would encourage the use of NGS technology for studying other hot springs in Indonesia.

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Screening of potential bioremediation enzymes from hot spring bacteria using conventional plate assays and liquid chromatography - Tandem mass spectrometry (Lc-Ms/Ms)

Cited by 36 publications

References 67 publications

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Unraveling the bacterial diversity of Cangar Hot Spring, Indonesia by Next Generation Sequencing of 16S rRNA gene

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