The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N(6)-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas aeruginosa, Salmonella enteritidis, and Streptococcus pyogenes. Antioxidant studies suggested that the polar subfractions of the methanol extract of intact plant and methanol extract of callus cultures were able to reduce the stable free radical 2,2-diphenyl-1-picrylhydrazyl to the yellow-colored diphenylpicrylhydrazine. In this assay, the strongest effect was observed for the tissue culture extract, with an IC(50) value of 23.76 +/- 0.80 microgram/mL, which could be compared with the synthetic antioxidant agent butylated hydroxytoluene. On the other hand, linoleic acid oxidation was 95% inhibited in the presence of the essential oil while the inhibition was 90% with the chloroform subfraction of the intact plant. The chemical composition of a hydrodistilled essential oil of S. hortensis was analyzed by gas chromatography (GC)/flame ionization detection (FID) and a GC-mass spectrometry system. A total 22 constituents representing 99.9% of the essential oil were identified by GC-FID analaysis. Thymol (29.0%), carvacrol (26.5%), gamma-terpinene (22.6%), and p-cymene (9.3%) were the main components.

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The aim of this study was to investigate in vitro antimicrobial and antioxidant activities of the methanol extracts of Cladonia foliacea Willd. Hudson, Dermatocarpon miniatum (L.) Mann., Everinia divaricata (L.) Ach., Evernia prunastri (L.) Ach., and Neofuscella pulla (Ach.) Essl. Antioxidant activity was evaluated by two separate methods: scavenging of free radical DPPH and the inhibition of linoleic acid oxidation. Extracts of C. foliacea, E. divaricata, E. prunastri, and N. pulla did not exert any activity in both assays, whereas those of D. miniatum provided 50% inhibition at 396.1 mg=ml concentration in the former and gave 49% inhibition in the latter. Total phenolic constituents of extracts from lichen species tested (C. foliacea, D. miniatum, E. divaricata, E. prunastri, and N. pulla) were 1.7% (w=w), 2.9% (w=w), 3.0% (w=w), 2.6% (w=w), and 1.5% (w=w), respectively (as gallic acid equivalent), implying that the observed activity could be related to the amount of polar phenolics. Extracts were also found to possess antimicrobial activity against some of the bacteria and fungi tested, but no activity was observed against the yeasts.

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A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position. Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus . The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences. Strain P1T could grow optimally at 55 °C and in the presence of 2 % NaCl. The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The predominant menaquinone was determined to be MK-7. The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus , for which the name Bacillus pasinlerensis sp. nov. is proposed. The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).

Myxosporeans of the genus Henneguya have a global distribution and infect organs and tissues of both marine and freshwater fishes. Here we describe the morphological, histological and molecular characteristics of Henneguya sinova sp. nov. parasitizing the gill arches of tentacled blenny Parablennius tentacularis (Perciformes: Blenniidae) collected from the coast of Sinop on the Black Sea in Turkey. Several oval whitish plasmodia of different sizes in the gill arches of fish were found. The mature spores were rounded oval in frontal view, with a mean (range) total length 57.5 (51.5-68.0) µm; the spore body was 11.7 (11.3-12.0) µm in length by 7.6 (7.3-8.3) µm in width and 6.7 (6.6-6.8) µm in thickness. The caudal appendages, measuring 46.0 (40.0-55.0) µm in length, were very thin at the tapered end. The prevalence of infection by H. sinova sp. nov. was 35.5%. Phylogenetic analysis of nuclear small subunit ribosomal DNA (SSU rDNA) clearly suggested H. sinova as a new species which is clustered within the marine Henneguya lineage. Pairwise nucleotide similarities and DNA distance values of SSU rDNA between H. sinova sp. nov. and other related Henneguya species also supported this suggestion.