Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Abstract

In this study, one hundred and thirty isolates were isolated from water and sludge samples taken from hot springs located in different regions of Turkey. Among them, eleven isolates were chosen according to conventional (morphological, physiological and biochemical tests) and molecular methods (rep-PCR and 16S rRNA sequencing). These bacteria were then tested for their capability to produce valuable enzymes. As a result; species belonging to Bacillus, Anoxybacillus, Aeribacillus, Enterococcus, Exiguobacterium and Paenibacillus were identified. Test strains were found to have optimum reproductive potential at pH 5.0-9.0 and 15-65°C, usually at a concentration of 1.0-10.0% (w/v) NaCl. In addition, all thermotolerant bacteria were Gram, endospore (except E. profundum), catalase and oxidase (except E. faecium and E. profundum) positive, and rod-shaped (except E. faecium). It was observed that all isolates had a 99% similarity percentage as a result of 16S rRNA sequence analysis. All of the isolates were capable of producing industrially important enzymes moreover, eight of them could produce at least two of these enzymes. Test strains had high potential of industrial enzyme production, and the enzymes from these thermo-tolerant isolates will be widely used in biotechnological processes.

Proteolytic, Lipolytic and Amylolytic Bacteria Reservoir of Turkey; Cold-Adaptive Bacteria in Detergent Industry

“…The bacterial isolates were inoculated on tributyrin agar (23 g/L) medium which contained 1% tributyrin (glycerol tributyrate) and incubated at 15°C for 3-5 days. The strains with transparent and the highest zone formation (lipolytic activity) were determined as lipase producers 16 . Amylase Amylase assay was performed at 15°C for 3-5 days in a medium which contained nutrient agar (20 g/L), soluble starch (5 g/L) and agar (15 g/L).…”

Bacteriocin Producing Bacteria Isolated from Turkish Traditional Sausage Samples

“…Afterwards, test isolates were subjected to rep-PCR [(GTG) 5 -PCR] with the special primer of (GTG) 5 elements to obtain genomic fingerprinting. The 16S rRNA gene regions of the isolates which would be considered to be different according to the rep-PCR analysis were amplified with universal primers by PCR and were subjected to the cloning procedure [13][14][15][16][17] .…”