Screening of Congenital CMV Infection in Saliva of Neonates by PCR: Report of a Pilot Screening Study in Iran

Fahimzad, Alireza; Afgeh, Seyyed Abolfazl; Eghbali, Elham; Abdinia, Babak; Shiva, Farideh; Rahbar, Mohammad

doi:10.7754/clin.lab.2013.120910

Cited by 9 publications

(9 citation statements)

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“…Our finding in this study is consistent with previous report from Tehran in which 0.32% of neonates (2 of 620) in a hospital were infected with CMV at birth ( 13 ). Additionally, similar findings were reported by two other studies with large sample sizes from Iran in which 0.49% and 0.65% of newborns in Isfahan and Gorgan cities (central and northern regions of Iran) were infected with CMV, respectively ( 14 , 15 ).…”

Section: Discussionsupporting

confidence: 93%

Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards

Noorbakhsh¹,

Farhadi²,

Haghighi³

et al. 2020

IJM

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Background and Objectives: Cytomegalovirus (CMV) constitutes the most common viral cause of congenital infections in newborns worldwide. There are a significant number of asymptomatic newborns with congenital CMV infection in Iran, which may develop long-term sequelae of infection. Unfortunately, limited data exsists from Iran on the rate of congenital CMV infection among neonates. The current study was aimed to investigate the prevalence of congenital CMV infection among Iranian neonates by testing Guthrie cards. Materials and Methods: Guthrie cards were collected from infants within 2 weeks of life, and total DNA was extracted from samples by thermal shock and evaluated for CMV DNA using nested-PCR assay. CMV infection in newborns was confirmed through a commercial CMV PCR kit. Infected infants underwent further evaluation at the hospital. Results: CMV infection was identified in four of 1174 infants (0.34%) which is approximately 3 cases per 1000 live births. Infected infants were asymptomatic at birth and had a normal hearing status similar to other children. There were no factors in relation with CMV infection among newborns. Conclusion: According to the results of this study, infected infants with congenital CMV infection could identify at early stage by testing Guthrie cards (within 21 days of life). Furthermore, since there is a lack of CMV knowledge in our popula- tion, educating and effective counseling by obstetricians/ gynecologists to the pregnant women are recommended.

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Section: Discussionsupporting

confidence: 93%

Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards

Noorbakhsh¹,

Farhadi²,

Haghighi³

et al. 2020

IJM

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“…To the best of our knowledge, the current study has provided the documented data for the first time in Iran on the rate of cCMV infection among neonates admitted to hospitals at birth by testing newborns' DBS cards and urine specimens. The overall prevalence rates of cCMV infection among asymptomatic and symptomatic neonates in Iran were reported within the range of 0.3-58% in total (16)(17)(18). In addition, similar findings were reported by a systematic review in which cCMV infection among the newborns of populations with high maternal CMV seroprevalence was reported to be about 6.1% (19).…”

Section: Discussionsupporting

confidence: 79%

Prevalence of Congenital Cytomegalovirus Infection Among Hospitalized Neonates in Tehran, Iran

Noorbakhsh

Farhadi

Rezaei

et al. 2020

ircmj

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Background: Cytomegalovirus (CMV) can vertically transmit from infected mothers to fetuses and causes congenital infection in newborns. Unfortunately, there have been limited data available on the prevalence of congenital CMV (cCMV) infection among Iranian neonates at higher risk of infection. Objectives: The current study aimed to assess the prevalence of cCMV infection among hospitalized neonates in Tehran, Iran, and investigate the diagnostic values of CMV polymerase chain reaction (PCR) on Guthrie cards in comparison to those reported for urine specimens. Methods: This cross-sectional study was carried out on the hospitalized neonates with 3 weeks of age. The urine specimens and Guthrie cards were taken from each eligible newborn. Total nucleic acid was extracted from the samples and tested by PCR for the presence of CMV deoxyribonucleic acid. The cCMV infection was confirmed in the newborns, and the infected neonates underwent further evaluation. Results: Out of 224 newborns, CMV infection was identified in 11 neonates (4.9%). There were no factors in association with cCMV infection. The sensitivity and specificity of dried blood spot (DBS) samples for the identification of newborns with cCMV infection were 90% and 99%, respectively. Conclusions: A significant number of hospitalized neonates in the present study were infected with cCMV infection. The results of the current study revealed that Guthrie cards had adequate sensitivity for the identification of CMV infection in the hospitalized newborns. Since symptomatic newborns with cCMV infection have a higher chance for the development of early- or late-onset sequelae of infection, it is recommended to diagnose and treat this group of newborns.

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“…A total of 77 articles were included in this meta-analysis. The final studies were from 36 countries (eFigure 3 in the Supplement ) on 5 continents and are categorized by World Health Organization regions as follows: Africa, 6 countries (6 studies) 30 , 31 , 32 , 33 , 34 , 35 ; Americas–Latin, 5 countries (9 studies) 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ; Americas–US and Canada, 2 countries (18 studies) 17 , 18 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 ; Eastern Mediterranean, 1 country (3 studies) 61 , 62 , 63 ; Europe, 16 countries (24 studies) 8 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ,…”

Section: Resultsmentioning

confidence: 99%

Congenital Cytomegalovirus Infection Burden and Epidemiologic Risk Factors in Countries With Universal Screening

et al. 2021

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Key Points Question What are the pooled prevalence of congenital cytomegalovirus infection and factors associated with the rates in high-income and low- and middle-income countries? Findings In this systematic review and meta-analysis including 77 studies from 36 countries comprising 515 646 infants younger than 3 weeks, the pooled overall prevalence of congenital cytomegalovirus was 0.67%. The infection burden was 3-fold greater in low- and middle-income countries than in high-income countries. Lower rates were reported in screening methods using blood compared with urine or saliva. Meaning The findings of this study suggest that low- and middle-income countries incur the greatest infection burden of congenital cytomegalovirus; a global effort to address congenital cytomegalovirus in regions with the greatest prevalence is needed to reduce disease incidence and morbidity.

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Screening of Congenital CMV Infection in Saliva of Neonates by PCR: Report of a Pilot Screening Study in Iran

Cited by 9 publications

References 0 publications

Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards

Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards

Prevalence of Congenital Cytomegalovirus Infection Among Hospitalized Neonates in Tehran, Iran

Congenital Cytomegalovirus Infection Burden and Epidemiologic Risk Factors in Countries With Universal Screening

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