Screening of Antibacterial, Thrombolytic, Membrane Stabilizing, Anti-inflammatory and Antitumor Activity of <i>Citrus assamensis</i> Leaf Extracts

Abstract

The methanol, ethanol and chlorofom leaf extracts of Satkara, Citrus assamensis (family: Rutaceae), were subjected to in vitro anti-bacterial, thrombolytic, membrane stabilizing and in vivo anti-inflammatory and antitumor activity tests. The chloroform extract of C. assamensis showed the most important spectrum of activity against Bacillus subtilis, Bacillus cereus, Sarcina lutea among 6 gram positive and against 11 gram negative bacteria at the concentration of 1000 μg/disc, while the range of zones of inhibition were within 7-16 mm. Among the tested three extracts CHCl 3 extract showed potent thrombolytic activity and hypotonic solution induced haemolytic activity where the percentages of inhibition were found to be 35% and 55% respectively. All the extracts established significant (p<0.05) anti-inflammatory effect by regulating biphasic inflammatory process induced by carrageenan. The leaf extract dose-dependently and significantly decreases the number of EAC cell count and inhibition of cell growth in comparison to the EAC control and standard. The results obtained in the present study indicate that, C. assamensis leaf can be a potential source of anti-bacterial, thrombolytic, membrane stabilizing, anti-inflammatory and antitumor agents.

In vitro Comparative Studies of the Leaf Extracts of Four Different Types of Calamus Species

“…18 The membrane stabilization by hypotonic solution and heat-induced haemolysis method was used to assess anti-inflammatory activity of the plant extracts by following standard protocol. 19 To prepare the erythrocyte suspension, whole blood was obtained from healthy human volunteer and was taken in syringes (containing anticoagulant 3.1% Na citrate). The blood was centrifuged and blood cells were washed three times with NaCl solution (154 mM) in 10 mM sodium phosphate buffer (pH 7.4) through centrifugation for 10 min at 3000 rpm.…”

Analysis of Proximate Composition and Functional Properties of Selected Edible Leaves: Nutritional and Therapeutic Implication

“…Anti-bacterial activities of leaf samples were evaluated using well diffusion method on nutrient agar against Gram positive Bacillus subtilis (MTCC 1305) and Gram negative Escherichia coli (MTCC 40). Antimicrobial property was determined by using a protocol reported by Shahriar et al [23] and the zone of inhibition (ZOI) was calculated against ethanol (negative control) to check the effectiveness of the extract. Chloramphenicol (20 µg/mL) was used as the positive control.…”

Antimicrobial activity of peels and physicochemical properties of juice prepared from indigenous citrus fruits of Sylhet region, Bangladesh

“…Citrus macroptera is a citrus fruit known as satkora in the region, and it is used for a variety of things, including cooking and as an odorant in the fragrance industry. Local communities in Assam, India, exploit semi-wild species mostly for medicinal purposes ( Shahriar et al., 2018 ). Citrus assamensis , commonly known as adajamir in Bangladesh, is the most prominent and costly citrus fruit cultivated in the larger Sylhet region.…”