Screening method for the addition of bovine blood‐based binding agents to food using liquid chromatography triple quadrupole mass spectrometry

Abstract

We report the development of a qualitative method to detect the addition of blood-based binding agents to food products. The method is based on the detection of species-specific marker peptides, fibrinopeptides, released from the blood protein fibrinogen during gelling of the blood protein by thrombin. The fibrinopeptides were isolated from foods spiked with commercial bovine binding agent by acid precipitation followed by enrichment using solid-phase extraction and analysed by liquid chromatography electrospray ionisation triple quadrupole mass spectrometry. Fibrinopeptide A was found to be an effective marker in fresh, processed and cooked food matrices spiked with 5% (v/w) bovine binding agent.

Identification of novel peptides for horse meat speciation in highly processed foodstuffs

“…Proteomics workflows have been developed in recent years using high-resolution MS to determine the species of origin of animal derived ingredients in food and feed products (Grundy et al 2007(Grundy et al , 2013Buckley et al 2009;Sentandreu & Sentandreu 2011;Reece et al 2012;Montowska et al 2014). Sentandreu et al (2010) investigated the use of proteomic approaches as an alternative to DNA-based methods for the determination of meat species in mixed-meat products.…”

A mass spectrometry method for the determination of the species of origin of gelatine in foods and pharmaceutical products

“…Mass spectrometry methods coupled to database searching can be used to authenticate and assess the composition of foods, additives and feeds using peptide markers which are publicly available (Grundy et al, 2007;Primrose, Woolfe, & Rollinson, 2010;Sentandreu, Fraser, Halket, Patel, & Bramley, 2010).…”

Plasma Darah dalam Makanan daripada Perspektif Islam dan Sains

“…Kromatografi cecair tri-caturkutub spektrometri jisim telah berjaya digunakan untuk menyaring penambahan agen pengikat berasaskan darah bovin (Grundy et al 2007) dan porsin (Grundy et al 2008) …”