Screening for anti-pancreatic lipase properties of 28 traditional Thai medicinal herbs

Dechakhamphu, Ananya; Wongchum, Nattapong

doi:10.1016/j.apjtb.2015.09.012

Cited by 50 publications

(34 citation statements)

References 21 publications

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“…Other studies published earlier also showed positive correlation between phenolic compounds and the antioxidant activity of plant extracts and pancreatic lipase inhibitory activity [33]. The present results demonstrate a significant positive correlation between phenolic contents and pancreatic lipase inhibition activity, pancreatic cholesterol esterase, and antioxidant activity (TEAC) which provide strong support that phenolic compounds are key agents for pancreatic lipase and pancreatic cholesterol esterase inhibition as well as antioxidant activity.…”

Section: Discussionsupporting

confidence: 86%

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Sompong

Muangngam

Kongpatpharnich

et al. 2016

BMC Complement Altern Med

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BackgroundObesity and overweight are consistently associated with metabolic disorders including hyperglycemia and hyperlipidemia. Herbal medicines have been currently suggested as an alternative source of potentially useful antihyperglycemic, antihyperlipidemic, antioxidant activities. The objective of this study was to assess the in vitro inhibitory effects of eleven herbal medicines on carbohydrate and lipid digestive enzymes and the key steps of lipid digestion including the inhibition of micelle formation and the ability to bind bile acid. In addition, antioxidant activity of herbal medicines was also investigated.Methodsα-Glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreatic cholesterol esterase inhibitory activities of aqueous extract of herbal medicines were measured using the enzymatic colorimetric assay. The formation of cholesterol micelles was determined using the cholesterol assay kit. The bile acid binding was measured using the colorimetric assay. Antioxidant activities were assessed by using four methods including Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity ORAC), superoxide radical scavenging activity (SRSA), and hydroxyl radical scavenging activity (HRSA).ResultsThe extracts (1 mg/mL) markedly inhibited intestinal maltase (5.16 − 44.33 %), sucrase (1.25–45.86 %), pancreatic α-amylase (1.75–12.53 %), pancreatic lipase (21.42–85.93 %), and pancreatic cholesterol esterase (2.92–53.35 %). The results showed that all extracts exhibited the inhibitory activity against pancreatic lipase with the IC50 values ranging from 0.015 to 4.259 mg/mL. In addition, the incorporation of cholesterol into micelles was inhibited by the extracts (6.64–33.74 %). The extracts also bound glycodeoxycholic acid (9.9–15.08 %), taurodeoxycholic acid (12.55–18.18 %), and taurocholic acid (11.91 − 18.4 %). Furthermore, the extracts possessed various antioxidant activities including the TEAC values (0.50 − 4.70 μmol trolox/mg dried extract), the ORAC values (9.14–44.41 μmol trolox/mg dried extract), the SRSA (0.31 − 18.82 mg trolox/mg dried extract), and the HRSA (0.05–2.29 mg trolox/mg dried extract). The findings indicated that Syzygium aromaticum, Phyllanthus amarus, Thunbergia laurifolia were the effective promising antihyperglycemic and antihyperlipidemic agents. Statistical analysis demonstrated strong positive significant correlations between the contents of phenolic compounds and % inhibition of pancreatic lipase (r = 0.885, p < 0.001), % inhibition of pancreatic cholesterol esterase (r = 0.761, p < 0.001), and the TEAC value (r = 0.840, p < 0.001). Furthermore, there was a strongly positive correlation between the TEAC value and % inhibition of pancreatic cholesterol esterase (r = 0.851, p < 0.001) and % inhibition of pancreatic lipase (r = 0.755, p < 0.001).ConclusionsThree herbal medicines including Syzygium aromaticum, Thunbergia laurifolia, and Phyllanthus amarus markedly inhibited the intestinal α-glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreat...

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Section: Discussionsupporting

confidence: 86%

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Sompong

Muangngam

Kongpatpharnich

et al. 2016

BMC Complement Altern Med

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“…In a similar mechanism as the Colocasia esculenta 's and the Abelmoschus esculentus ' mucilage (Chukwuma, Islam, & Amonsou, ) Cymodocea nodosa 's mucilage (Zhang, Yang, Liang, Jiao, & Zhao, ) and related Ziziphus mauritana (Deshpande et al, ) and Ziziphus jujuba 's mucilage (Ganachari, Kumar, & Alagawadi, ), Nabq mucilage (Table ) diminished lipid absorption at the intestinal level by inhibiting the activity of pancreatic lipase (Elbashir et al, ), scoring as the second best inhibitor among 18 dietetic plants. Dechakhamphu and Wongchum (), as Tan et al () went deeper to arraign plant phenolics (within identical mucilages) as potent inhibitors of carbohydrate digesting enzymes, namely phenolic acids like ferulic acid (and other phenolic acids like p‐coumarin, p‐hydroxybenzoic acid, and syringic acid or phenolic esters like chlorogenic acid); Ghafoor, Qadir, and Fakhri () found ferulic acid in the NabM as well as phenolic compounds like rutin, apigenin, and quercetin. High phenolic diet also plays a role in stabilizing and modulating antioxidants from milk protein, improving their prophylactic effect against the oxidative stress (Xing et al, ).…”

Section: Resultsmentioning

confidence: 99%

Dietary solutions to dyslipidemia: Milk protein–polysaccharide conjugates as liver biochemical enhancers

et al. 2020

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Protein–polysaccharide interactions have been a focus of scientific attention. This study aimed to improve the antioxidant and hepatoprotective effects of buffalo total milk proteins and whey protein hydrolysate (WPH) through noncovalent interactions with Psyllium husk (ispaghula) mucilage (PHM) and Nabq mucilage (NabM). Chemical composition, phenolic content, and antioxidant activities of milk protein–mucilage complexes were explored. The effects of resulting complexes on liver function, hyperlipidemia, and histopathology of the liver in rats fed high‐fat diet (HFD) were investigated. The results showed that the complexes exerted significant effect on normalizing tested parameters; WPH–NabM had the most significantly decreased level of malondialdehyde content and the liver histopathological examination proved an improvement in all groups fed with these complexes. These complexes can be used as functional protection elements against the nonalcoholic fatty liver disease. Practical applications PHM, NabM, and their complexes with milk proteins were proved to improve liver function, enhancing most of its measurable parameters and also diminishing the risk of cardiovascular disease. Mice with HFD achieved better health circumstances by combining these ingredients in their diet. Knowing how much these diseases proliferate in the western world and its correlation with high‐fat consumption and modern lifestyle, its conjugation with PHM/NabM–MP complexes may reduce the negative impact of unhealthy food intake and, on some parameters, even improving the whole liver function. For that reason, the present study supports and pushes forward the dissemination and consumption of ispaghula or Nabq (the polysaccharides sources) or supplements originating from them. Although many interactions concerning milk proteins have already been analyzed, our study also proposes the interaction with bioactive polysaccharides as useful, opening a field of research aimed at the better application of milk proteins.

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“…The lipase inhibitory activity of Camellia mistletoe extracts was estimated from the ability to inhibit the in vitro porcine pancreatic lipase activity as previously described [9] with slight modifications. The reaction mixture consisted of 6 μL of 10 mg/ml of porcine pancreatic lipase and 170 μL of Tris buffer.…”

Section: Pancreatic Lipase Inhibitory Activitymentioning

confidence: 99%

Inhibitory Activities of Camellia Mistletoe (K. Japonica) Extracts on Pancreatic Lipase, Tyrosinase and Cancer Cell Proliferation

Kim

2017

Int J Pharm Pharm Sci

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Objective: The objective of this study was undertaken to examine pancreatic lipase and tyrosinase inhibitory activities of Camellia mistletoe (Korthalsella japonica) extracts and conduct their antiproliferative activity on several human cancer (Hela, A375, HCT 116, HepG2 and A549) and normal cells (TK6) lines. Methods:The lipase inhibitory activity of acts of Camellia mistletoe extracts were determined from the hydrolytic reaction of p-nitrophenyl butyrate with pancreatic lipase and cytotoxicity against human cancer and normal cell lines were also evaluated using the MTT assay. The in vitro tyrosinase inhibitor potency of Camellia mistletoe extracts was estimated using mushroom tyrosinase.Results: Both methanol and ethanol extracts of Camellia mistletoe exhibited an inhibitory effect on lipase inhibition activity with IC50 values of 0.39 and 0.49 mg/ml, respectively. Moreover, Camellia mistletoe extracts were judged to be strongly active against the cancer cell lines, while they had no antiproliferative activity against the normal human cell lines. However, they had little inhibitory effects on tyrosinase activity. Conclusion:Camellia mistletoe extracts could be the good sources of natural lipase inhibition and anticancer agents with a great potential to be used in food and therapeutic fields.

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Screening for anti-pancreatic lipase properties of 28 traditional Thai medicinal herbs

Cited by 50 publications

References 21 publications

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Dietary solutions to dyslipidemia: Milk protein–polysaccharide conjugates as liver biochemical enhancers

Inhibitory Activities of Camellia Mistletoe (K. Japonica) Extracts on Pancreatic Lipase, Tyrosinase and Cancer Cell Proliferation

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