The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Sompong, Weerachat; Muangngam, Nuttapat; Kongpatpharnich, Artitaya; Manacharoenlarp, Chadakarn; Amorworasin, Chanatkarn; Suantawee, Tanyawan; Thilavech, Thavaree

doi:10.1186/s12906-016-1424-2

Cited by 27 publications

(19 citation statements)

References 58 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In previous studies, alkaloids and terpenoids were reported to inhibit α-glucosidase activity and α-amylase. [26][27] Likewise, these findings synchronized with the report of Sompong et al and Riyaphan et al [28][29] in which some plants extract inhibited carbohydrate and lipid metabolism enzymes activities.…”

Section: Discussionsupporting

confidence: 82%

In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Encephalartos ferox leaves

Ojo¹,

Osunsanmi²,

Zaharare³

et al. 2019

View full text Add to dashboard Cite

Diabetes Mellitus (DM) is a progressive metabolic disorder of carbohydrates and lipids that is characterized by hyperglycemia. 1 DM is known to result from defect in insulin secretion, action or both. 2 Poor management of diabetes mellitus can degenerate into debilitating conditions including heart attack, stroke, kidney failure, leg amputation, vision loss, nerve damage and erectile dysfunction. 1 The inability of the cells to assimilate glucose formed via stepwise catabolism of carbohydrates with α-amylase and α-glucosidase, leads to hyperglycemia. Likewise, hyperactivity of pancreatic lipase results to increase in lipid metabolism which also triggers hyperlipidemia. 3-4 Therefore, hyperglycemia and hyperlipidemia continue to be the underlying factors in the onset of diabetes mellitus and its complications. 5 In addition, reactive oxygen species also play crucial role in the pathogenesis of diabetes mellitus. 6 Despite the potency of currently used diabetic drugs such as acarbose, Voglibose, Miglitol, insulin mimetics and secretagogues, they still are associated with adverse side effects. 7 Therefore, the search for alternative In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Encephalartos ferox leaves ABSTRACT Background: Diabetes mellitus has been identified as one of the global cause of disability and death. Objectives: The study aim to investigate the in-vitro antidibetic and antioxidant activities of methanolic extract of Encephalartos ferox leaves. Materials and Methods: The plant was screened for its Phytochemical composition. The plant material was extracted with methanol and the methanolic extract was screened (in-vitro) for its antioxidant activity using ABTS and DPPH assays. The potential antidiabetic activity of the plant extract was evaluated against some carbohydrates (α-amylase and α-glucosidase) and lipid (pancreatic lipase) digestive enzymes. The inverted intestinal sac model was also used to investigate the effect of the extract on intestinal glucose absorption. The anti-protein glycation activity of the extract was determined using haemoglobin. Results: The phytochemical screening revealed the presence of most of the phytochemicals (Tannins, Flavonoids, Terpenoids, Alkaloids etc) that were screened for. The crude extract exhibited the antidiabetic potential as it significantly (P < 0.05) inhibited α-glucosidase and pancreatic lipase in a dose dependent fashion. The extract also effectively reduced intestinal glucose absorption. The extract further showed antioxidant activity by efficiently scavenging ABTS and DPPH radicals with IC 50 values of 68.3 µg/ml and 308 µg/ml, respectively. The extract also inhibited haemoglobin glycation, thus displaying the anti-protein glycation potential. Conclusion: It is apparent that E. ferox extract could serve as scaffold for diabetic therapy. For future study, cytotoxicity profile and in vivo investigation of the antidiabetic activity of the crude extract are essential.

show abstract

Section: Discussionsupporting

confidence: 82%

In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Encephalartos ferox leaves

Ojo¹,

Osunsanmi²,

Zaharare³

et al. 2019

View full text Add to dashboard Cite

show abstract

“…& Thonn., Thunbergia laurifolia Lindl at concentration of 1 mg/mL were confirmed by investigating the inhibition not only of the pancreatic lipase activity (21.42-85.93%), but also pancreatic cholesterol esterase (2.92-53.35%), intestinal maltase (5.16-44.33%), sucrase (1.25-45.86%), and pancreatic α-amylase activity (1.75-12.53%). In addition, inhibition of the cholesterol incorporation into micelles (6.64-33.74%), as well as, the bile acids binding (2.05-18.40%) was established (Sompong et al, 2016). Among 10 investigated Asteracea species Argyranthemum pinnatifidum Lowe var.…”

Section: In Vitro Studiesmentioning

confidence: 99%

“…Current strategies for screening potent natural anti-obesity drugs involve the study of organic or water extracts of different parts (roots, leaves, fruits, flowers or seeds) of the medicinal plants, as well as, some related phytochemicals such as polyphenols, terpenoids, alkaloids, saponins, steroids and polysaccharides for their antihyperlipidemic, antihyperglycemic, carbohydrate and fat digestion reduction properties supported by their ability to modulate the molecular pathways and gene/protein expressions responsible of these activities (de Freitas Junior and de Almeida, 2017; Sompong et al, 2016;Spínola and Castilho, 2017). The suppression of the digestive enzymes, especially the pancreatic and gastric lipases, suppression of AMPK/malonyl Co-A signaling pathway or potential ghrelin antagonism that blunt the increased appetite or mediate the reduced expression of hypothalamic NPY or serum leptin (Yun, 2010), the increase of the energy expenditure via stimulating thermogenesis in muscles and BAT through the activation of UCP1 with natural compounds (Song et al, 2017b;Stohs and Badmaev, 2016) are the most widely studied mechanisms for determining natural products' beneficial effect in overweight and obese people (Sompong et al, 2016).…”

Section: Traditional Medicinal Plants As Powerful Source Of Novel Antmentioning

confidence: 99%

“…Hundreds of plant species have been screened for their activity to inhibit pancreatic lipase (Seyedan et al, 2015;Tung et al, 2017) and some other digestive enzymes (α-amylase, αand β-glucosidase; Sompong et al, 2016) or to increase insulin sensitivity, supported with investigations of the molecular signaling pathways in several model cell lines, such as 3T3-L1, HEPG2 or L6 cells (Pan et al, 2017;Semaan et al, 2018). The present review summarizes some recent studies of plant extracts or pure isolated compounds with in vitro activity similar to that of the referenced drugs (e.g.…”

Section: In Vitro Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Causes and solutions to “globesity”: The new fa(s)t alarming global epidemic

Vasileva

Marchev

Georgiev

2018

Food and Chemical Toxicology

View full text Add to dashboard Cite

Diverse groups of factors are leading to increased weight gain and obesity, such as certain genetic phenotypes, neuroendocrine disturbances, the administration of some drugs, behavioral, social and environmental factors. The progressively escalating rates of overweight and obesity worldwide have led to an introduction of a new term "globesity". Excessive accumulation of body fat and especially of visceral adipose tissue is the main predisposing factor for the development of metabolic syndrome and other obesity related co-morbidities. At the present moment only few pharmacotherapeuticals are used for long-term treatment of obesity acting on narrow target spectra, e.g. pancreatic and gastric lipase inhibition, acting as adrenomimetics or activating the satiety centers in hypothalamus. Plant-based medications that accelerate weight loss, proved to be safe, effective and widely available, would be a preferable alternative for anti-obesity treatments. As plant extracts are multi-component systems they could also act by more than one mechanism, including decreased lipid absorption, decreased energy intake, increased energy expenditure, decreased pre-adipocyte differentiation and proliferation, decreased lipogenesis and increased lipolysis. The current review gives a summary of the risk factors for obesity development and its characteristics consequences. Current treatment options, combining lifestyle changes and conventional treatment with commercial anti-obesity drugs have been described as well. Special emphasis on in vitro, in vivo and human studies, of potential medicinal plant extracts and phytochemicals, such as polyphenols, terpenoids, alkaloids, saponins, able to modulate the molecular pathways and gene/protein expressions related to obesity, have been highlighted.

show abstract

“…Ezzel szemben a gyógynövények α-amiláz és α-glükozidáz inhibitorainak kevésbé káros hatásai vannak (Kumar, Narwal et al 2011, Satoh, Igarashi et al 2015. Korábban számos természetes növényi eredetű antidiabetikus termékeket vizsgáltak, melyek egy része hatásos α-glikozidáz és α-amiláz inhibitor volt (Hung, Qian et al 2012, Sompong, Muangngam et al 2016. Az általunk vizsgált növényi kivonatok in vitro α-glükozidáz gátlásának középértékei μg/ml-es tartományba estek, amelyek szignifikánsan alacsonyabbak, mint a fekete tea levelek 28 mg/ml IC50 értékei (Satoh, Igarashi et al 2015) vagy a Zingiber 3 mg/ml gátló koncentrációi (Jo, Cho et al 2016), de magasabbak, mint az ayurvédikus gyógyászatban cukorbetegségben használt gyógynövény a Saliac oblongata 4 μg/ml IC50 értéke (Li, Peng et al 2004) és a Prunica granatum 1,8 μg/ml IC50 értéke (Li, Wen et al 2005).…”

Section: éRtékelésunclassified

Szénhidrátbontó enzimek gátlásának vizsgálata STZ indukált diabetes mellitus egérmodellben

Takács¹

View full text Add to dashboard Cite

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Cited by 27 publications

References 58 publications

In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Encephalartos ferox leaves

In-vitro Anti-diabetic and Antioxidant Efficacy of Methanolic Extract of Encephalartos ferox leaves

Causes and solutions to “globesity”: The new fa(s)t alarming global epidemic

Szénhidrátbontó enzimek gátlásának vizsgálata STZ indukált diabetes mellitus egérmodellben

Contact Info

Product

Resources

About