Screening Bacterial and Radical Scavenging Properties of Chosen Plant Extracts Using Thin-Layer Chromatography–direct Bioautography

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

“…TLC seems to be an ideal method for hyphenation with bioautographic detection because of an open layer enabling solvent evaporation, and the possibility of separating many samples in parallel. Besides classical TLC, high-performance thin-layer chromatography (HPTLC), overpressured-layer chromatography (OPLC) and planar electro-chromatography (PEC) can also be easily linked to bioautography [12][13][14][15][16][17][18].…”

“…The tests based on these bacteria were fully validated in our laboratory and used for screening antibiotics in milk [19][20][21][22]. Recently, we have also started to use them in plant analysis [13,14,23]. Antibacterial properties of plant constituents were checked against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Xanthomonas campestris pv.…”

“…Besides searching for bioactive compounds, TLC-DB can serve other purposes, such as finding the best solvent for extraction of active compounds from a given matrix [13,26,27,53], or selection of a mobile phase providing a suitable separation of investigated samples [14,26,27]. TLC-DB also allows comparison of bioactivity for samples of various origin in parallel (e.g., different plant species or extracts from the different parts of the plant) [13,14,26,27,53].…”