Separation, Identification, and Investigation of Antioxidant Ability of Plant Extract Components Using TLC, LC–MS, and TLC–DPPH^•

Abstract

Ten common phenolic compounds from five plant extracts i.e. Hypericum perforatum L., Matricaria recutita L., Achillea millefolium L., Thymus vulgaris L. and Salvia officinalis L. were separated using optimized TLC conditions and visualized using NP/PEG reagent.Antioxidant properties of components of the extracts were assessed using TLC-DPPH • directly on the TLC plate. Nine from ten analyzed polyphenols were proved to be radicalscavengers. The presence and identity of target substances in plant samples were confirmed by LC-MS technique.

Quality control of Hypericum perforatum L. analytical challenges and recent progress

“…However, there are some TLC studies which analysed the phenolic content of Hypericum species, including the study of Jesionek et al . and Males et al …”

“…The silica gel TLC plates were developed with seven different mobile phases and then derivatized with NP reagent and PEG reagent. The research team found that flavonoid aglycons like quercetin were better separated with the system toluene : diethyl ether : acetic acid (60 : 40 : 10 v/v/v), the flavonoid glycosides like rutin and hyperoside with the system ethyl acetate : acetic acid : formic acid : water (100 : 11 : 11 : 26 v/v/v/v) and the phenolic acids like chlorogenic acid with the system chloroform : ethyl acetate : acetone : formic acid (40 : 30 : 20 : 10 v/v/v/v) …”

Antioxidant Capacity Determination in Plants and Plant‐Derived Products: A Review

“…Four other compounds (caffeic acid and apigenin in St. John wort and apigenin and apigenin 7-O-glucoside in sage) have been identified. Their presence was not revealed by TLC and it has been stated that their low level in the plant samples could be the reason [91]. …”

Preparative separation of grape skin polyphenols by high-speed counter-current chromatography

“…Traditional separation methods for polyphenols such as liquid-liquid extraction (Sun, Ferrão, & Spranger, 2003;Sun et al, 2006), thin-layer chromatography (Jesionek, Majer-Dziedzic, & Choma, 2015;Sun, Leandro, Ricardo-da-Silva, & Spranger, 1998), column chromatography (Lu & Foo, 1999;Sun, Belchior, Ricardo-da-Silva, & Spranger, 1999), semi-preparative and preparative HPLC (Makhotkina & Kilmartin, 2012;Sun, Belchior, et al, 1999) have disadvantages like timeconsuming, secondary-pollution, complex-process, low-yield and high-cost. High-speed counter-current chromatography (HSCCC), a supporting free liquid-liquid partition system where solutes are partitioned between two immiscible phases, offers various advantages such as high solute loading capability, high recovery, high repeatability and low solvent consumption (Zhang et al, 2015).…”