Antimicrobial, DPPH scavenging and tyrosinase inhibitory activities of <i>Thymus vulgaris, Helichrysum arenarium</i> and <i>Rosa damascena mill.</i> ethanol extracts by using TLC bioautography and chemical screening methods

Akın, Mustafa; Şaki, Neslihan

doi:10.1080/10826076.2019.1591977

Cited by 18 publications

(7 citation statements)

References 36 publications

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“…Turkish scientists have proven the antimicrobial activity of the alcohol extract of Rosa damascena Mill. using disk diffusion and well diffusion methods against Escherichia coli (ATCC 25922) bacteria, as well as inhibitory activity against tyrosinase according to TLC-bioautography data [11].…”

Section: Discussion Of the Resultsmentioning

confidence: 99%

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Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation

Odyntsova,

Denysenko,

Shkopynska

et al. 2023

SR: PS

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Damask rose of the Veselka variety is an important industrial rose variety used to obtain essential oil. It is widely used in modern cosmetology, perfumery, and aromatherapy. In addition, the essential oil of Damask rose has a whole spectrum of pharmacological properties. The scientific innovation of this research lies in its foundation on cultivating and acquiring planting materials of the Damask rose in vitro. This approach guarantees controlled conditions for plant growth, the production of robust seedlings, and an enhancement in the precision and credibility of the research outcomes. Moreover, the study has scientific novelty in that it explores to assess both the quantitative and qualitative constituents of the essential oil in the acquired plant material of the Damask rose. This assessment takes place within the context of cultivating regenerative plants in an outdoor environment. Such an approach acknowledges the potential distinctions in the oil's component composition acquired from plants propagated through this method in comparison to traditional vegetative reproduction. Lastly, the research has scientific novelty in investigating the potential antimicrobial properties of Damask rose essential oil, which could have significant practical implications in the development of new drugs and combatting infectious diseases. The purpose of the study was to establish the component composition of the essential oil of Rosa damascena Mill., which was grown in vitro, and to determine its antimicrobial effect. Methods. The object of the study was the essential oil of Damask rose of the Veselka variety, which was grown by the method of clonal micropropagation in vitro. The essential oil was extracted from fresh petals collected during dry weather conditions prior to sunrise by hydrodistillation. Determination of the qualitative composition and quantitative content of volatile substances was carried out by the GC-MS method using an Agilent 7890B chromatograph. Antimicrobial activity was studied in vitro using the disk diffusion method with reference test strains of microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Candida albicans ATCC 885-653. Results. According to the results of the chromato-mass spectrometric study, 41 compounds (6 of which were in the isomeric state) were identified, which was belong to 13 different classes of chemicals. Dominant compounds among terpenoid substances were shown: geraniol – 30.96 %, citronellol – 27.08 %, alkanes: nonadecane – 17.29 %, and heneicosene – 5.46 %. It was established that the essential oil of Damask rose had a significant antimicrobial effect against strains of C. albicans and E. coli, the diameters of which growth retardation zones ranged from 32-35 mm and 20-23 mm, respectively. In studies with P. aeruginosa and S. aureus, the essential oil showed moderate antibacterial activity: the diameters of the growth retardation zone of these microorganisms ranged from 13 to 15 mm and 11 to 12 mm, respectively. Conclusion. For the first time, the qualitative composition and quantitative content of volatile substances in the essential oil extracted from the petals of Rosa damascena Mill., Veselka variety, cultivated through the clonal micropropagation in vitro, were explored by chromatography-mass spectrometry techniques. The results of the study of antimicrobial activity showed that the studied essential oil exhibits significant fungicidal effects against Candida microorganisms, along with moderate bactericidal effects on gram-negative (E. coli, P. aeruginosa) and gram-positive (S. Aureus) bacteria. These results highlight the potential of this essential oil for further investigation in the realm of developing novel medicines and herbal preparations. Further clinical studies are needed to assess this potential.

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Section: Discussion Of the Resultsmentioning

confidence: 99%

“…It should be noted the presence of antimicrobial and anti-inflammatory action. For example, in an article by Turkish scientists [11], the effect of an alcohol extract against Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). However, the antimicrobial effect was confirmed only against E. coli.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation

Odyntsova,

Denysenko,

Shkopynska

et al. 2023

SR: PS

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“…LBKURCC34 dilakukan uji KLT-Bioautografi seperti yang pernah dilakukan Owen et al (2019). Metode KLT-Bioautografi umum digunakan karena efektif untuk mempelajari senyawa aktif dan memberikan informasi pendahuluan tentang aktivitas, lokasi aktivitas antimikroba, dan efikasi dari senyawa atau noda langsung pada KLT (Armengol et al 2021;Akin & Saki 2019). Metode ini cocok dan dapat diterima untuk penentuan in situ pada antimikroba farmasetikal (Nuthan et al 2020).…”

Section: Hasil Dan Pembahasanunclassified

Identifikasi Metabolit Sekunder Sekresi Jamur Lokal Tanah Gambut Riau Penicillium sp. LBKURCC34 Sebagai Antimikroba

2022

CNA

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Penicillium sp. LBKURCC34 adalah jamur yang diisolasi dari tanah gambut hutan primer di Giam Siak Kecil Bukit Batu (GSKBB), Cagar Biosfer di Provinsi Riau. Tujuan dari penelitian ini adalah untuk mengeksplorasi potensinya sebagai penghasil antimikroba. Metode dilakukan melalui produksi metabolit sekunder dalam media cair, ekstraksi dalam larutan etil asetat, dan pemisahan ekstrak kasar dengan Kromatografi Kolom (CC). Ekstrak kasar dan fraksi diidentifikasi dengan KLT dan HPLC dan diuji aktivitas antimikrobanya dengan metode difusi cakram. Untuk mengidentifikasi senyawa aktif dilakukan uji fitokimia dan metode KLT-Bioautografi. Produksi tiga liter media menghasilkan 0,4 gram (0,0133% b/v) ekstrak etil asetat yang diidentifikasi sebagai terpenoid dan fenolat; dan mengandung senyawa semipolar berdasarkan uji KLT dan HPLC. Hasil KLT setelah disemprot dengan p-anisaldehida 0,5% menunjukkan adanya senyawa peptaibol (peptida). Ekstrak etil asetat dapat menghambat pertumbuhan kelima mikroba patogen yang diuji namun hanya 11-45% dibandingkan dengan kontrol positif. Uji bioautografi terhadap Escherichia coli menunjukkan noda aktif zona bening pada Rf sebesar 0,67. Sayangnya, dari Fraksi 1-5 yang terdapat noda dengan Rf 0,67 masih belum menunjukkan aktivitas berarti. Penyelidikan lebih lanjut diperlukan untuk mengidentifikasi struktur senyawa aktif dari isolat lokal ini.

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“…Thymus vulgaris ethanol extract has demonstrated a very good antioxidant capacity and an antibacterial effect (Akin, & Saki, 2019). These two qualities are required to preserve food from rancidity and microbiological degradation.…”

Section: Introductionmentioning

confidence: 99%

Use of essential oils for food safety: Formulations of easy-to-use food-grade spray sanitizers

Rim,

Rabarisoa,

Harizafy

et al. 2023

Food & Feed Res

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Motivated by combat against the spread of foodborne diseases, the formulations of simple and easy-to-use spray disinfectants containing Cinnamomum zeylanicum and Ocimum gratissimum essential oils were studied. Central composite experimental design was used in the development of stable products with optimal essential oil content for antimicrobial efficacy. It also allowed minimizing the alcohol content which was used to ensure miscibility between the essential oils and the aqueous phase. Trans-cinnamaldehyde was the major compound in the C. zeylanicum essential oil. Its low toxicity allowed the formulation of a risk-free product despite the great variability in the chemical composition of the essential oil. Cooked food coated with a spray containing 1% of this essential oil, in combination with Thymus vulgaris tincture, could be preserved for 3 days without refrigeration. Eugenol was the major component of O. gratissimum essential oil, followed by 1,8cineole and methylchavicol. Sprays containing 1.5% of this essential oil were effective against Escherichia coli and Staphylococcus aureus. Xanthan gum proved to be a good essential oil-trapping agent under accelerated ageing conditions at 45 °C.

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Antimicrobial, DPPH scavenging and tyrosinase inhibitory activities of Thymus vulgaris, Helichrysum arenarium and Rosa damascena mill. ethanol extracts by using TLC bioautography and chemical screening methods

Cited by 18 publications

References 36 publications

Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation

Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation

Identifikasi Metabolit Sekunder Sekresi Jamur Lokal Tanah Gambut Riau Penicillium sp. LBKURCC34 Sebagai Antimikroba

Use of essential oils for food safety: Formulations of easy-to-use food-grade spray sanitizers

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