TNF‐α triggers osteogenic differentiation of human dental pulp stem cells via the NF‐κB signalling pathway

Abstract

Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells (MSCs) characterised by self-renewal and multi-lineage differentiation, including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for tissue engineering purposes. Tumour necrosis factor α (TNF-α) had the positive effect on the mineralisation of bone marrow MSCs and stromal cells derived from human adipose tissue. However, the effect of TNF-α on DPSCs is unclear. We found that TNF-α activated the NF-κB pathway during the osteogenic differentiation of DPSCs. TNF-α also increased mineralisation and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and collagen type I (COL I) during this process. PDTC, an NF-κB inhibitor, blocked the osteogenic differentiation induced by TNF-α. No effect of TNF-α on proliferation of DPSCs or cell cycle was detected. In summary, TNF-α promotes mineralisation and mineralisation-related gene expression through the NF-κB signalling pathway in DPSCs, which may provide a foundation for autologous transplantation of DPSCs.

Expression of thymosin beta-4 in human periodontal ligament cells and mouse periodontal tissue and its role in osteoblastic/cementoblastic differentiation

“…The data presented are representative of three independent experiments. NF-κB signaling (Feng et al, 2013). These conflicting observations may result from differences in the experimental conditions or cell types.…”

Protective effects of resveratrol on postmenopausal osteoporosis: regulation of SIRT1-NF-κB signaling pathway

“…Among them, NF-kB is a well-known molecule involved in various metastatic bone diseases [25]. It has been reported that NF-kB signaling pathway is an important Effects of resveratrol on postmenopausal osteoporosis mediator in osteoblast differentiation [26,27]. Previous studies also reported that constitutive inhibition of NF-kB in Saos2 cells could increase the expression of osteoblast specific matrix protein collagen I and promote osteoblast differentiation [28,29].…”

Mucus of Achatina fulica stimulates mineralization and inflammatoryresponse in dental pulp cells

“…Earlier researchers showed that stem cells from tooth pulp can develop into bone and this bone formation activity may be useful for osseointegrated dental implants (Yamada et al, 2010). DPSCs are a type of MSCs characterized by selfrenewal and multilineage differentiation, making them an attractive choice for tissue engineering purposes, which may provide a foundation for autologous transplantation of DPSCs (Feng et al, 2013a(Feng et al, , 2013b.…”