PM 7/119 (1) Nematode extraction

Abstract

Specific scope This standard describes procedures for nematode extraction. Specific approval and amendment Approved in 2013‐09.

Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea

“…Mixed populations (TB372) were sampled from Chinese cabbage fields of Taebaek. Cysts were extracted from infested field soil samples and Chinese cabbage roots using modified Cobb’s decanting and sieving method (EPPO, 2013). Cysts were recovered from a 250 μm sieve and filtered through Whatman ® 150 mm filter paper (GE Healthcare Life Sciences, Marlborough, USA) before handpicking using fine forceps under a stereomicroscope Nikon SMZ 1000 (Nikon, Tokyo, Japan).…”

“…Cysts were recovered from a 250 μm sieve and filtered through Whatman ® 150 mm filter paper (GE Healthcare Life Sciences, Marlborough, USA) before handpicking using fine forceps under a stereomicroscope Nikon SMZ 1000 (Nikon, Tokyo, Japan). Infective second stage juveniles (J2s) were extracted from the soil using a modified Baermann funnel method (EPPO, 2013). …”

Alleviation of Nematode-Mediated Apple Replant Disease by Pre-Cultivation of <em>Tagetes</em>

“…These microbes were shown to synergistically enhance ARD symptoms together with the nematode fraction, but they had hardly any effect when inoculated to apple plantlets alone [31]. While floatation is a standard technique to retrieve nematodes from soil [48], and the fraction on the 20 µm-sieve mainly contains nematodes when microscopically analysed, it might contain other small organisms and microbes associated with small organic particles, which may play a role in ARD. This needs to be ruled out in future studies.…”

Taxonomy, Morphological and Molecular Identification of the Potato Cyst Nematodes, Globodera pallida and G. rostochiensis

“…The Fenwick can, as modified by Oostenbrink [19], is the most commonly used instrument for the extraction of cysts from soil samples using the principles of flotation [43,44]. Nematode cysts are relatively light in relation to the inorganic fraction of soil, have a waxy covering, and contain a pocket of air within, so it is possible to separate cysts in the lighter organic fraction of the soil for identification and assessment.…”