Pistacia atlantica leaf extract mediated synthesis of silver nanoparticles and their antioxidant, cytotoxicity, and antibacterial effects under in vitro condition

Abstract

Recently, researchers have investigated the therapeutical properties of metal nanoparticles especially silver nanoparticles in vitro and in vivo conditions. The aim of the experiment was green synthesis and chemical characterization of silver nanoparticles from aqueous extract of Pistacia atlantica leaf (Ag NPs) and evaluation of their cytotoxicity, antioxidant, and antibacterial effects under in vitro condition. Ag NPs were spherical with a size range of 40‐60 nm and characterized using various analysis techniques including UV–Vis absorption spectroscopy to determine the presence of Ag NP in the solution. We studied functional groups of Pistacia atlantica extract in the reduction and capping process of Ag NP by FT‐IR, crystallinity and FCC planes by XRD pattern, elemental analysis of the sample by EDS, and surface morphology, shapes, and size of Ag NPs by SEM, AFM, and TEM. Destroy initiation and termination temperatures of the Ag NPs were determined by TGA. DPPH free radical scavenging test was done to evaluate the antioxidant potentials, which indicated similar antioxidant potentials for Ag NPs and butylated hydroxytoluene. The synthesized Ag NPs had great cell viability dose‐dependently and indicated this method was nontoxic. Agar diffusion tests were done to determine the antibacterial characteristic. Ag NPs revealed similar antibacterial property to the standard antibiotic. Also, Ag NPs prevented the growth of all bacteria at 1‐7 μg/ml concentrations and removed them at 3‐15 μg/ml concentrations. Finally, synthesized Ag NPs revealed non‐cytotoxicity, antioxidant and antibacterial activities in a dose‐depended manner.

Exploiting Fruit Waste Grape Pomace for Silver Nanoparticles Synthesis, Assessing Their Antioxidant, Antidiabetic Potential and Antibacterial Activity Against Human Pathogens: A Novel Approach

“…The distinction in affectability of Gram-positive and Gram-negative microorganisms to GPE-AgNPs was because of the thickness and the constituents of their membrane structure [33]. The obtained results are found similar with the earlier studies concerning AgNPs synthesized using biological materials [9,52].…”

<p>Chlorhexidine Nanoemulsion: A New Antiseptic Formulation</p>

“…30 Organic nanoparticles bring promising results, highlighting nanoemulsions that may contain antimicrobial drugs, essential oils with antimicrobial activity, or even the combination of both. [31][32][33][34][35][36][37] Numerous studies bring results when it comes to the use of inorganic nanoparticles using different plant extracts with antimicrobial and healing activities, which include silver nanoparticles, [38][39][40][41][42] titanium nanoparticles, 43,44 gold nanoparticles, [45][46][47] copper nanoparticles, 48,49 zinc nanoparticles, 50 iron nanoparticles 51 and manganese nanoparticles. 52 The aim of this study was to evaluate and validate an aqueous-based chlorhexidine nanoemulsion at 0.25% (NM-Cl).…”

<p>Dental Materials Incorporated with Nanometals and Their Effect on the Bacterial Growth of <em>Staphylococcus aureus</em></p>

“…This result is in agreement with previous studies that used agar disk-diffusion to demonstrate the susceptibility of S. aureus to metallic nanoparticles. [4][5][6][7] However, the inhibitory extent of silver nanoparticles depends to a greater degree on the synthesis approach used, and thus the diameter of the inhibition zone against S. aureus exhibits a variety of concentrations. [4][5][6][7] Furthermore, instead of paper disks, dental porcelain incorporated with one kind of metallic nanoparticle was evaluated by Hashem et al 25 using the disk diffusion, and the authors found that the growth of S. aureus was inhibited effectively.…”

“…Researchers favor natural sources in the synthesis of metallic nanoparticles, mainly silver nanoparticles. [4][5][6][7] Metallic nanoparticles are made either of a single element or a combination of elements, eg, nanoparticles can be made solely of silver or a combination of silver and copper elements. Silver nanoparticles can be shaped differently, eg, spheres or rods.…”

“…8 Various approaches and techniques have been developed for the synthesis of nanomaterials to make them one of the most applicable and widely used materials in science. For example, silver nanoparticles are used as biocides and antioxidants [4][5][6][7]9 and utilized either alone or integrated into other structures/substances. Additionally, silver nanoparticles have biomedical and pharmaceutical applications, such as cancer treatment and medical imaging, 10,11 not to mention other uses in engineering, cosmetics, and agriculture.…”