HPLC with quadrupole TOF‐MS and chemometrics analysis for the characterization of Folium Turpiniae from different regions

Abstract

Folium Turpiniae has been used as a traditional Chinese medicine for the treatment of abscesses, fevers, gastric ulcers, and inflammations. This paper describes a strategy of combining HPLC with photodiode array detection and quadrupole TOF-MS, as well as phytochemical and chemometrics analysis for the characterization, isolation, and simultaneous quantification of the chemical constituents of Folium Turpiniae. 19 constituents were identified, namely, 11 flavonoids, seven gallic acid derivates, and quinic acid. Among them, 15 compounds were identified in this herbal medicine for the first time; compound 10 appears to be novel and was isolated and confirmed as ellagic acid-3-O-α-L-rhamnopyranoside by NMR spectroscopy and MS. In addition, nine marker compounds, namely gallic acid (2), ellagic acid-3-O-α-L-rhamnopyranoside (10), apigenin-7-O-(2''-rhamnosyl)gentiobioside (11), ellagic acid (12), luteolin-7-O-β-D-neohesperidoside (13), ligustroflavone (14), 4'-O-methylellagic acid-3-O-α-L-rhamnopyranoside (16), rhoifolin (17), and neobudofficide (18), were quantified simultaneously in ten batches of Folium Turpiniae collected from different regions. Moreover, hierarchical clustering analysis and principal component analysis indicated that both samples from Hubei (S1) and Guangxi (S3) provinces showed apparent differences from the others. Samples from Jiangxi province (S2, S4, and S7-10) possessed similar properties and therefore belong to the same group.

Applications of High Performance Liquid Chromatography in the Analysis of Herbal Products

“…Folium Turpiniae, a well-known traditional Chinese medicine derived from Turpinia arguta (Staphyleaceae), is used for the treatment of abscesses, fevers, gastric ulcers, and inflammations [84]. A strategy incorporating HPLC-PDA and quadrupole TOF-MS detection, as well as phytochemical and chemometrics analysis (and fingerprinting) for the characterization, isolation, and simultaneous quantification of the chemical constituents of Folium Turpiniae has recently been reported [84].…”

Analysis of the traditional medicine YiGan San by the fragmentation patterns of cadambine indole alkaloids using HPLC coupled with high‐resolution MS

“…It is valuable but difficult to identify and assign most constituents in a complex herbal medicine. Fortunately, such is the sensitive and reliable approach developed for quantifying seven alkaloids in Fuzi decoction by triple‐quadrupole MS , and the application of quadrupole TOF MS (Q‐TOF‐MS) in identifying multiple constituents of Folium Turpiniae , HPLC–MS/MS has been proved rapid and sensitive with greater accuracy and precision for structural elucidation and multiple component quantification in complex systems . Therefore, an explanation on the whole picture of chemical component and the content of key compounds in YGS is necessary and feasible.…”

Identification of the Major Components of Buddleja officinalis Extract and Their Metabolites in Rat Urine by UHPLC‐LTQ‐Orbitrap

“…Fragmentation of the molecular ion of component 16 ( m/z 737.2262, 12.61 min, –3.446 ppm) led 3 major product ions at m/z 591.1711 ([M‐H‐Rha] − ), 283.0614 (the [M‐H] − ion of luteolin) and 240.0435 ([M‐H‐2Rha‐Glc‐CH 3 ‐CO] − ). By comparison with the published literature, component 16 was tentatively identified as neobudofficide (Li and others ).…”