Identification of the Major Components of <i>Buddleja officinalis</i> Extract and Their Metabolites in Rat Urine by UHPLC‐LTQ‐Orbitrap

Sun, Mohan; Luo, Zhiqiang; Liu, Yang; Yang, Ruirui; Liu, Lu; Yang, Guohua; Ma, Xiaoyun; Liu, Aoxue; Guo, Yafang; Zhao, Haiyu

doi:10.1111/1750-3841.13435

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…Phytochemical analysis of Buddleja extracts identified polyphenols, flavonoids and phenylethanoid as major components [45, 46]. Buddleja showed antimicrobial activity against Bacillus subtilis, S. aureus, E. coli and K. pneumoniae supporting the traditional use of the plant in the treatment of eye infections [28].…”

Section: Discussionmentioning

confidence: 99%

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

El-Ganiny

Shaker

Aboelazm

et al. 2017

J Ophthal Inflamm Infect

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PurposeIn eye care field, contact lenses (CL) have a great impact on improving vision, but their use can be limited by ocular infection. CL- associated infections can be reduced by good attention to CL storage case practice. CL-care solutions should be able to control microbial growth on CL.The aim of the study was to evaluate and compare the efficacy of CL-care solutions (found in Egyptian market) with some natural compounds in removal and inhibition of bacterial biofilm formed on soft CL.Clinical isolates were recovered from patients having conjunctivitis from Benha University Hospital and identified microbiologically. Quantification of biofilm was done using microtiter plate assay. Three multipurpose CL-care solutions were examined for their ability to remove and inhibit biofilm. Also four natural extracts having antibacterial activity and are safe on eye were tested for their anti-biofilm activity.ResultsThe major bacterial isolates from eye infections were Pseudomonas aeruginosa (36%) and Staphylococcus spp. (37.8%). Only 33.3% of isolates showed ability to produce weak to moderate biofilm. The tested multi-purpose CL-care solutions showed moderate ability to remove preformed biofilm. Among the tested natural compounds, Calendula officinalis and Buddleja salviifolia extracts showed an excellent efficacy in inhibition of biofilm and also removal of preformed biofilm.ConclusionThis study demonstrated that isolates from infected eye and CL-cases showed weak to moderate biofilm formation. Calendula officinalis and Buddleja salviifolia extracts showed excellent effect on inhibition and removal of biofilm, these extracts could be added into CL-care solutions which could markedly reduce eye-infections during CL-wear.

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Section: Discussionmentioning

confidence: 99%

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

El-Ganiny

Shaker

Aboelazm

et al. 2017

J Ophthal Inflamm Infect

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“…However, the determination method by HPLC usually took too much time (>40 min), and was not sensitive enough for trace component analysis in the complicated extracts. Recently, a hybrid linear ion trap-Orbitrap (LTQ-Orbitrap) analytical platform has been applied to the analysis of small molecules in various traditional Chinese medicine (TCM) and biological samples [13][14][15][16][17]. It consists of a 2D ion trap coupled with an Orbitrap, and allows two different scan types to be acquired simultaneously.…”

Section: Optimum Conditions For Uhplc-dad-ltq-orbitrap Ms Analysismentioning

confidence: 99%

Simultaneous Qualitation and Quantitation of Chlorogenic Acids in Kuding Tea Using Ultra-High-Performance Liquid Chromatography–Diode Array Detection Coupled with Linear Ion Trap–Orbitrap Mass Spectrometer

et al. 2016

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Kuding tea, the leaves of Ilex Kudingcha C.J. Tseng, has been applied for treating obesity, hypertension, cardiovascular disease, hyperlipidemia, and so on. The chlorogenic acids (CGAs) in Kuding tea have shown excellent antioxidative, antiobesity, anti-atherosclerotic and anticancer activities. Nevertheless, the chemical profiles of CGAs in Kuding tea have not been comprehensively studied yet, which hinders further quality control. In the present study, a sensitive ultra-high-performance liquid chromatography-diode array detection coupled with a linear ion trap-Orbitrap (UHPLC-DAD-LTQ-Orbitrap) method was established to screen and identify CGAs in Kuding tea. Six CGA standards were first analyzed in negative ion mode with a CID-MS/MS experiment and then the diagnostic product ions (DPIs) were summarized. According to the retention behavior in the RP-ODS column, accurate mass measurement, DPIs and relevant bibliography data, a total of 68 CGA candidates attributed to 12 categories were unambiguously or preliminarily screened and characterized within 18 min of chromatographic time. This was the first systematic report on the distribution of CGAs in Kuding tea. Meanwhile, the contents of 6 major CGAs in Kuding tea were also determined by the UHPLC-DAD method. All the results indicated that the established analytical method could be employed as an effective technique for the comprehensive and systematic characterization of CGAs and quality control of the botanic extracts or Chinese medicinal formulas that contain various CGAs.

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“…Hence, the overall objective of this research was to investigate in detail the in vivo absorption and metabolism of Areca catechu L. nut extract (ACNE). As we all know, plasma and urine are recognized as ideal biological matrices in drug absorption and metabolism studies [ 21 , 22 ]. In this work, a total of 26 components in the plasma and 31 components in the urine were characterized by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap).…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of the Metabolites in Rat Plasma and Urine after Oral Administration of Areca catechu L. Nut Extract by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap–Orbitrap Tandem Mass Spectrometry

Luo

Yang

et al. 2017

Molecules

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Areca catechu L. nut, a well-known toxic traditional herbal medicine, has been widely used to treat various diseases in China and many other Asian countries for centuries. However, to date the in vivo absorption and metabolism of its multiple bioactive or toxic components still remain unclear. In this study, liquid chromatography coupled with tandem mass spectrometry was used to analyze the major components and their metabolites in rat plasma and urine after oral administration of Areca catechu L. nut extract (ACNE). A total of 12 compounds, including 6 alkaloids, 3 tannins and 3 amino acids, were confirmed or tentatively identified from ACNE. In vivo, 40 constituents, including 8 prototypes and 32 metabolites were identified in rat plasma and urine samples. In summary, this study showed an insight into the metabolism of ACNE in vivo, which may provide helpful chemical information for better understanding of the toxicological and pharmacological profiles of ACNE.

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Identification of the Major Components of Buddleja officinalis Extract and Their Metabolites in Rat Urine by UHPLC‐LTQ‐Orbitrap

Cited by 10 publications

References 27 publications

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

Simultaneous Qualitation and Quantitation of Chlorogenic Acids in Kuding Tea Using Ultra-High-Performance Liquid Chromatography–Diode Array Detection Coupled with Linear Ion Trap–Orbitrap Mass Spectrometer

Screening and Identification of the Metabolites in Rat Plasma and Urine after Oral Administration of Areca catechu L. Nut Extract by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap–Orbitrap Tandem Mass Spectrometry

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