Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

Abstract

PurposeIn eye care field, contact lenses (CL) have a great impact on improving vision, but their use can be limited by ocular infection. CL- associated infections can be reduced by good attention to CL storage case practice. CL-care solutions should be able to control microbial growth on CL.The aim of the study was to evaluate and compare the efficacy of CL-care solutions (found in Egyptian market) with some natural compounds in removal and inhibition of bacterial biofilm formed on soft CL.Clinical isolates were recovered from patients having conjunctivitis from Benha University Hospital and identified microbiologically. Quantification of biofilm was done using microtiter plate assay. Three multipurpose CL-care solutions were examined for their ability to remove and inhibit biofilm. Also four natural extracts having antibacterial activity and are safe on eye were tested for their anti-biofilm activity.ResultsThe major bacterial isolates from eye infections were Pseudomonas aeruginosa (36%) and Staphylococcus spp. (37.8%). Only 33.3% of isolates showed ability to produce weak to moderate biofilm. The tested multi-purpose CL-care solutions showed moderate ability to remove preformed biofilm. Among the tested natural compounds, Calendula officinalis and Buddleja salviifolia extracts showed an excellent efficacy in inhibition of biofilm and also removal of preformed biofilm.ConclusionThis study demonstrated that isolates from infected eye and CL-cases showed weak to moderate biofilm formation. Calendula officinalis and Buddleja salviifolia extracts showed excellent effect on inhibition and removal of biofilm, these extracts could be added into CL-care solutions which could markedly reduce eye-infections during CL-wear.

In VitroBiofilm Formation and Antibiotic Susceptibility Patterns of Bacteria from Suspected External Eye Infected Patients Attending Ophthalmology Clinic, Southwest Ethiopia

“…e biofilm formation rate found in this study is comparable with a study conducted in Chicago [44]. However, the comparatively lower biofilm formation rate was reported in Saudi Arabia [45]. P. aeruginosa (80%), K. pneumoniae (77.8%), CoNS (75.6%), S. aureus (72.4%), and E. coli (60%) were among the leading biofilm formers.…”

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

“…), Pseudomonas on contact lenses (El‐Ganiny et al . ) and Staphylococci in orthopaedic implants and breast implants (Arciola et al . ; Seng et al .…”

Hydrogel Delivery System Containing Calendulae flos Lyophilized Extract with Chitosan as a Supporting Strategy for Wound Healing Applications

“…[75]. Calendulae flos extracts have well documented antimicrobial activity [19][20][21], especially polyphenols and essential oil, are useful in wound healing [76,77]. As shown in Table 4, it is noteworthy that the combination of the Calendulae flos lyophilized extract with chitosan did not exhibit a lower MBC value, in particular against pathogens responsible for wound infections.…”

“…The positive effect on the wound healing process has been proven in numerous in vitro and in vivo studies, which were found to increase the proliferation and migration of human fibroblast and keratinocytes, stimulating angiogenesis and decrease collagen degradation after use of Calendulae flos extracts [14][15][16][17][18]. Furthermore, the Calendulae flos extract possesses some pharmacological features, which include antioxidant and anti-inflammatory action, as well as antibacterial, antifungal, and antiviral activity against various pathogens, e.g., Staphylococcus aureus, Escherichia coli, Bacillus cereus, and others [19][20][21].…”