Scouring of cotton using marine pectinase

Joshi, Manasi; Nerurkar, Madhura; Badhe, Pallavi; Adivarekar, Ravindra V.

doi:10.1016/j.molcatb.2013.10.010

Cited by 23 publications

(15 citation statements)

References 14 publications

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“…[15] Media used for growth and fermentative production of pectinase are as mentioned earlier. [16] After 24 hr the broth contents were centrifuged at 5000 rpm at 4 C for 20 min and the cell-free supernatant obtained was used as the crude enzyme sample.…”

Section: Microorganism Culture Conditions and Pectinase Productionmentioning

confidence: 99%

Characterization, Kinetic, and Thermodynamic Studies of Marine Pectinase FromBacillus subtilis

Joshi

Nerurkar

Adivarekar

2014

Preparative Biochemistry and Biotechnology

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Characterization, kinetic and thermodynamic parameters of purified pectinase from Bacillus subtilis, isolated from a marine sediment sample collected from Chinchani beach at Tarapore, India, were studied. Marine pectinase produced under submerged growth conditions was purified by ammonium sulfate precipitation followed by gel filtration chromatography using DEAE cellulose. Partial characterization of the marine pectinase was carried out in terms of effect of pH, temperature, substrate concentration, and metal ions. It was found that pectinase from marine B. subtilis showed maximal activity in alkaline buffer at pH 9.0 and at 40°C. It was also found that metal ions, namely, Mn(2+) and Fe(2+), stimulate pectinase activity. Marine pectinase followed Michaelis-Menten kinetics. The kinetics and thermodynamic parameters of the purified marine pectinase from B. subtilis were studied as the characterization of the enzyme is vital for its use in industrial processes.

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Section: Microorganism Culture Conditions and Pectinase Productionmentioning

confidence: 99%

Characterization, Kinetic, and Thermodynamic Studies of Marine Pectinase FromBacillus subtilis

Joshi

Nerurkar

Adivarekar

2014

Preparative Biochemistry and Biotechnology

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“…Cotton bioscouring could be as much effi cient as the conventional alkaline treatment in terms of physicochemical properties such as, wettability, whiteness index, polymerization degree, crystallinity index, color depth, brightness, softness, absorbency, weight loss as well as low-stress mechanical properties [26,27,28].…”

Section: Bioscouringmentioning

confidence: 99%

Enzymatic treatments for cotton

Mojsov¹,

Janevski²,

Andronikov³

et al. 2020

Tekstilna industrija

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Textile processing is a growing industry that traditionally has used a lot of water, energy and harsh chemicals. Conventional chemical processes are generally severe and fi bre damage may occur. Enzymes are characterized by their ability to operate under mild conditions and as a result processes can be carried out without further damaging the fi bers. Enzymes are also readily biodegradable and therefore potentially harmless and environmentally friendly. Furthermore, the use of enzymes results in reduced process times, energy and water savings, improved product quality. This work represents a review of current research and future directions on the applications of enzymatic treatments for cotton. The enzymes used in the textile fi eld are amylases for desizing of cotton and cellulases for denim fi nishing and biopolishing. The application of cellulases for denim fi nishing and laccases for decolourization of textile effl uents and textile bleaching are the most recent commercial advances.

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“…The use of microbial enzymes for textile fiber processing has increased significantly, as they cause less damage to fibers, improve fabric quality, require less energy and use less environment-damaging chemicals. (Chiliveri and Linga 2014;Dhiman et al 2008;Joshi et al 2013;Mojsov 2016;Shanthi and Krishnabai 2013). Bioscouring produces good quality fibers with more softness and better strength.…”

Section: Introductionmentioning

confidence: 99%

A sustainable and green process for scouring of cotton fabrics using xylano-pectinolytic synergism: switching from noxious chemicals to eco-friendly catalysts

et al. 2018

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The objective of this research was to develop an appropriate, eco-friendly, cost-effective bioscouring methodology for removing natural impurities from cotton fabric. Maximum bioscouring was achieved using 5.0 IU xylanase and 4.0 IU pectinase with material to liquid ratio of 1:15 in a 50 mM buffer (glycine-NaOH buffer, 1.0 mM EDTA and 1% Tween-80, pH 8.5) with a treatment time of 60 min at 50 °C and an agitation speed of 60 rpm. The bioscoured cotton fabrics showed a gain of 1.17% in whiteness, 3.23% in brightness and a reduction of 4.18% in yellowness in comparison to fabric scoured with an alkaline scouring method. Further, after bleaching, the whiteness, brightness and tensile strength of the bioscoured fabrics were increased by 2.18, 2.33 and 11.74% along with a decrease of 4.61% in yellowness of bioscoured plus bleached fabrics in comparison to chemically scoured plus bleached fabrics. From the results, it is clear that bioscouring is more efficient, energy saving and an eco-friendly process and has the potential to replace the environment-damaging scouring process with the xylano-pectinolytic bioscouring process.

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Scouring of cotton using marine pectinase

Cited by 23 publications

References 14 publications

Characterization, Kinetic, and Thermodynamic Studies of Marine Pectinase FromBacillus subtilis

Characterization, Kinetic, and Thermodynamic Studies of Marine Pectinase FromBacillus subtilis

Enzymatic treatments for cotton

A sustainable and green process for scouring of cotton fabrics using xylano-pectinolytic synergism: switching from noxious chemicals to eco-friendly catalysts

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