Schwangerschaftsprotein 1 (Sp1) as a Maternal Serum Marker for Down Syndrome in the First and Second Trimesters

Qin, Qiuping; Christiansen, Michael; Nguyen, Tam T. T. N.; Sørensen, Steen; Larsen, Severin Olesen; Nørgaard‐Pedersen, Bent

doi:10.1002/(sici)1097-0223(199702)17:2<101::aid-pd4>3.0.co;2-h

Cited by 32 publications

(26 citation statements)

References 22 publications

(29 reference statements)

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“…SP1 (Qin et al, 1997;Brizot et al, 1995;Wald et al, 1999b), has a 'window of uselessness' (Brizot et al, 1995) located somewhere between 11 to 13 weeks.…”

Section: Discussionmentioning

confidence: 99%

ADAM 12 as a second‐trimester maternal serum marker in screening for Down syndrome

et al. 2007

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Background ADAM 12 is a placenta-derived glycoprotein that is involved in growth and differentiation. The maternal serum concentration of ADAM 12 is a potential first-trimester maternal serum marker of Down syndrome (DS). Here we examine the potential of ADAM 12 as a second-trimester maternal serum marker of DS. Materials and MethodsThe concentration of ADAM 12 was determined in gestational week 14-19 in 88 DS pregnancies and 341 matched control pregnancies. Medians of normal pregnancies were established by polynomial regression and the distribution of log 10 MoM ADAM 12 values in DS pregnancies and controls determined. Correlations with alpha-fetoprotein (AFP) and free β-human chorionic gonadotrophin (free β-hCG) were established and used to model the performance of maternal serum screening with ADAM 12 in combination with other second-trimester serum markers. ResultsThe ADAM 12 maternal serum concentration was significantly increased with a median MoM of 1.85 and a mean log 10 MoM (SD) of 0.268 (0.2678) compared to a mean log 10 MoM (SD) of 0.013 (0.4318) in controls. ADAM 12 correlated with maternal weight and ethnicity (with the serum concentration increased in Afro-Caribbeans), but neither with maternal age nor gestational age, and only marginally with AFP (r(DS) = 0.078, r(controls) = 0.093) and free β-hCG (r(DS) = 0.073, r(controls) = 0.144. The increase in detection rate-for a false positive rate of 5%-by adding ADAM 12 to the double test (AFP + free β-hCG) was 4%, similar to that of adding uE3 to the double test.Conclusion ADAM 12 is an efficient second-trimester marker for DS. Further studies should be conducted to determine whether it may be a useful additonal or alternative marker to those currently used in the secondtrimester.

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“…SP1 (Qin et al, 1997;Brizot et al, 1995;Wald et al, 1999b), has a 'window of uselessness' (Brizot et al, 1995) located somewhere between 11 to 13 weeks.…”

Section: Discussionmentioning

confidence: 99%

ADAM 12 as a second‐trimester maternal serum marker in screening for Down syndrome

et al. 2007

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“…For example, PAPP-A levels are significantly reduced in maternal serum from Down's syndrome pregnancies in the first trimester but are virtually unchanged in the second trimester Berry et al, 1997). SP-1 levels are reduced in the first trimester and elevated in the second trimester Qin et al, 1997) while PALP levels show little or no variation in either the first or the second trimester (Brock et al, 1990;Ind et al, 1994;Aitken et al, 1996a).…”

Section: Discussionmentioning

confidence: 99%

“…Placental extracts (diluted 500) were assayed for intact hCG using an immunoradiometric assay (Serono MAIA clone) and undiluted extracts assayed for SP-1 by immunoelectrophoresis (Graham et al, 1992). The SP-1 assay was modified for placental extracts by doubling the sample volume and reducing the volume of antibody in the antiserum strip to 25 l. SP-1 was measured in amniotic fluid by immunofluorometric assay (Qin et al, 1997). GGT was measured by spectrophotometric kinetic assay (Sigma) following dilution of the samples in distilled water (neat for placenta, 10 for liver, and 2 for ileum).…”

Section: Marker Analysesmentioning

confidence: 99%

Biochemical markers of trisomy 21 and the pathophysiology of Down's syndrome pregnancies

Newby¹,

Aitken²,

Crossley³

et al. 1997

Prenat. Diagn.

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“…SP1 is synthesized by the syncytiotrophoblast and secreted into the maternal circulation, where the concentration increases continuously during pregnancy. The SP1 concentration in maternal serum is a marker of Down syndrome, with a reduced concentration noted early in the first trimester and an increased concentration in the second trimester (3,4 ). Furthermore, serum SP1 is decreased in pregnancies with fetal growth restriction.…”

Section: To the Editormentioning

confidence: 99%

“…One approach has been to use criteria for change obtained from the 2007 universal myocardial infarction (MI) definition (1 ). This was recently used in a comparison of a sensitive cTnI assay [AccuTnI; refer to Apple (3 ) for assay designation] against a research thirdgeneration hs assay (hs-cTnI) for early detection of myocardial injury (4 ). In the present study, we assessed if the criteria for change used in the previous study (4 ) also add to risk stratification with this hs-cTnI assay.…”

Section: To the Editormentioning

confidence: 99%

Pregnancy-Specific β-1-Glycoprotein in First-Trimester Maternal Serum Is Influenced by Smoking

Pihl

Christiansen

2010

Clinical Chemistry

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PEG precipitation has limitations of which users of this technique should be aware (5 ), and gel filtration chromatography remains the gold standard. Nevertheless, PEG precipitation remains the most practical approach available for screening for macroprolactinemia. We believe that in the interests of clarity and good laboratory practice, it is time to abandon reporting results as percentage macroprolactin in favor of the absolute post-PEG prolactin concentration, together with an appropriate reference interval. In differentiating between true hyperprolactinemia and macroprolactinemia, this approach will serve to facilitate interpretation of the result, provide more clinically useful information, and enhance patient care.

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Schwangerschaftsprotein 1 (Sp1) as a Maternal Serum Marker for Down Syndrome in the First and Second Trimesters

Cited by 32 publications

References 22 publications

ADAM 12 as a second‐trimester maternal serum marker in screening for Down syndrome

ADAM 12 as a second‐trimester maternal serum marker in screening for Down syndrome

Biochemical markers of trisomy 21 and the pathophysiology of Down's syndrome pregnancies

Pregnancy-Specific β-1-Glycoprotein in First-Trimester Maternal Serum Is Influenced by Smoking

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