Schizosaccharomyces pombe

Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

“…Haploid wild-type S. pombe strains h À 972 and h 975 (Gutz et al 1974) and their derivative mutant strains were used. The double mutants between mis3-224 (Takahashi et al e1994) and various mutations noted below were constructed; cdc2-33, cdc6-121, cdc13-117, cdc25-22, cdc10-129 (Nurse et al 1976), cdc2-3w (Enoch et al 1992), Dcyc17/cig2 (Obara-Ishihara & Okayama 1994), Dmik1 (Lundgren et al 1991), wee1-50 (Russell & Nurse 1987), cdc16-116 (Fankhauser et al 1993), cdc17-K42, cdc18-K46 (Nasmyth & Nurse 1981), cdc22-C1 (Fernandez Sarabia et al 1993), swi7-H4 (Singh & Klar 1993), mis5-268, mis11-453 (Takahashi et al 1994, Drad3, Drad9, Drad17, Drad24, Drad25, Dchk1 (Al-Khodairy et al 1994), cut5-T401 (Saka et al 1994), Dcds1 (Murakami & Okayama 1995), Ddis1 (Nabeshima et al 1995), dis3-54 (Kinoshita et al 1991), dis2-11, Ddis2 (Ohkura et al 1989), cut1-21, cut2-364 (Funabiki et al 1996, cut9 (Yamada et al 1997), Dppa1, Dppa2 (Kinoshita et al 1993),Dpck1 (Toda et al 1993), Dtop1, top2-191 (Uemura & Yanagida 1984), and Ddsk1 mutants (Takeuchi & Yanagida 1993).…”

The Schizosaccharomyces pombe mra1 gene, which is required for cell growth and mating, can suppress the mating inefficiency caused by a deficit in the Ras1 activity

“…Crosses of mating-deficient strains were performed by protoplast fusion (Sipiczki & Ferenczy 1977). Media used in this study have been described (Egel & Egel-Mitani 1974;Gutz et al 1974;Watanabe et al 1988). Mating and sporulation frequencies were calculated as previously described (Kunitomo et al 1995).…”

“…Leu + transformants were grown on SSA plates, which contain only a limited amount of nitrogen and permit cells to initiate sexual development within a colony at the end of vegetative growth. Transformants that could mate efficiently were identified by staining colonies with iodine vapour (Gutz et al 1974). Dark-stained colonies were inspected under the microscope to confirm the formation of zygotes and asci.…”

Schizosaccharomyces pombe U4 small nuclear RNA closely resembles vertebrate U4 and is required for growth

“…pombe have been described by Gutz et al (1974) . The diploid strain used for gene disruption earries h+ ,leul.32/h+,leul.32 ;urar Dl8/urar Dl8;ade 6.704/ade 6.704 …”

“…To obtain haploid spores, rare h 9°/ h+ revertants were identified (Gutz et al, 1974), which switch mating type to h-jh+ and sporulate. Amongst 2700 haploid spores, none was found to carry the inserted LEU+ marker, demonstrating that snu4 is essential, at least for spore germination.…”