Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Kondoh, Eiji; Yuasa, Tatsuro; Yanagida, Mitsuhiro

doi:10.1046/j.1365-2443.2000.00344.x

Cited by 14 publications

(10 citation statements)

References 79 publications

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“…If fission yeast Cti1 is also involved in ribosomal DNA processing, a fundamental requirement for cellular growth, then it would not be surprising that the disruption of cti1 ϩ led to the inability to germinate and the inhibition of growth as observed. Moreover, the localization and phenotypes of cti1 null mutant resembles closely that caused by mutation in Mis3 (20), a K-homologydomain-containing protein essential for the processing of rRNA. The nucleolar region might, at the same time, also act as a reservoir of Cti1, and in the presence of DNA damage signals, Cti1 might be released and redistributed to the spots of action.…”

Section: Discussionmentioning

confidence: 86%

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Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin

Chen

Sutani²,

Yanagida³

2004

Proc. Natl. Acad. Sci. U.S.A.

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Condensin is a conserved five-subunit complex containing two SMC (structural maintenance of chromosomes) and three non-SMC subunits and plays a major role in mitotic chromosome condensation. Condensin also acts in interphase and is required for DNA repair and replication checkpoint control. We attempted to study the function of the condensin in greater detail by means of the isolation of interacting proteins with the two-hybrid system. Using the hinge domain of Cut3͞SMC4 as bait, we found one Cut three-interacting (Cti) 14-kDa nuclear protein, Cti1. GST pull-down assay and immunoprecipitation supported physical interaction between Cti1 and condensin. Cti1 is similar to human C1D, which associates tightly with genomic DNA and functions to activate DNA protein kinase. SpC1D is essential for viability. The null mutant could germinate but arrest after replication, indicating that it is required for interphase growth. Importantly, an elevated dosage of spC1D suppressed the temperature, UV irradiation, and hydroxyurea sensitivity of the mutant of Cnd2, a non-SMC subunit of condensin. Upon exposure to hydroxyurea, spC1D accumulated on the nuclear chromatin, and the fraction of spC1D that was chromatin-bound increased. Cti1 is the first example of the protein that interacts with the hinge domain of SMC. Cti1 may have a supporting role for the DNA repair function of condensin.

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Section: Discussionmentioning

confidence: 86%

“…3D). Replication seemed to occur Ϸ13 h after germination, like the timing in wild-type cells (20). Hence, the terminal phenotypes of cti1 null appeared to be the inability to grow and divide.…”

Section: Cti1 Interacts With Cut3 In Vitro and In Vivomentioning

confidence: 90%

Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin

Chen

Sutani²,

Yanagida³

2004

Proc. Natl. Acad. Sci. U.S.A.

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“…4B, right, and details described in Supplementary Table S2). The three up-regulated (UP1-UP3) gene clusters are related to ribosome biogenesis (Kondoh et al 2000), protein biosynthesis, and RNA polymerase I, RNA binding, processing, translation factors, tRNA modification and nucleases. The gene products are present in the cytoplasm, nucleolus and nuclear lumen.…”

Section: Genes Related To Ribosome Biogenesis Transcription and Tranmentioning

confidence: 99%

Two‐step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe

et al. 2007

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Body cells in multicellular organisms are in the G0 state, in which cells are arrested and terminally differentiated. To understand how the G0 state is maintained, the genes that are specifically expressed or repressed in G0 must be identified, as they control G0. In the fission yeast Schizosaccharomyces pombe, haploid cells are completely arrested under nitrogen source starvation with high viability. We examined the global transcriptome of G0 cells and cells on the course to resume vegetative growth. Approximately 20% of the transcripts of ~5000 genes increased or decreased more than fourfold in the two-step transitions that occur prior to replication. Of the top 30 abundant transcripts in G0, 23 were replaced by ribosome-and translation-related transcripts in the dividing vegetative state. Eight identified clusters with distinct alteration patterns of ~2700 transcripts were annotated by Gene Ontology. Disruption of 53 genes indicated that nine of them were necessary to support the proper G0 state. These nine genes included two C2H2 zinc finger transcription factors, a cyclin-like protein implicated in phosphorylation of RNA polymerase II, two putative autophagy regulators, a G-protein activating factor, and two CBS domain proteins, possibly involved in AMP-activated kinase.

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“…This result pinpoints Leu-95 as a key site for Kri1 association. A temperature-sensitive mutant of S. pombe Krr1, mis3-224, contains a single Gly to Glu mutation (41). The equivalent residue of yeast Krr1, Gly-175, is an invariant residue located at a FIGURE 3.…”

Section: Structure Determination Of the Krr1 And Faf1mentioning

confidence: 99%

Interaction between Ribosome Assembly Factors Krr1 and Faf1 Is Essential for Formation of Small Ribosomal Subunit in Yeast

Zheng

Lan

Liu

et al. 2014

Journal of Biological Chemistry

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Background:The 90 S precursor of small ribosomal subunits is an enormous and poorly understood structure. Results: A subcomplex structure of 90 S proteins Krr1 and Faf1 was determined. Conclusion: The KH domains of Krr1 are versatile in protein interaction, and the Krr1-Faf1 interaction is essential for forming functional 90 S pre-ribosomes. Significance: The study provides structural and functional insight of a protein-protein interaction within 90 S pre-ribosomes.

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Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint

Abstract: Background: In normal somatic cell cycle, growth and cell cycle are properly coupled. Although CDK (cyclin-dependent kinase) activity is known to be essential for cell cycle control, the mechanism to ensure the coupling has been little understood.

Cited by 14 publications

References 79 publications

Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin

Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin

Two‐step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe

Interaction between Ribosome Assembly Factors Krr1 and Faf1 Is Essential for Formation of Small Ribosomal Subunit in Yeast

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