Schizosaccharomyces pombe U4 small nuclear RNA closely resembles vertebrate U4 and is required for growth

Dandekar, Thomas; Ribes, Véronique; Tollervey, David

doi:10.1016/0022-2836(89)90502-0

Cited by 17 publications

(6 citation statements)

References 35 publications

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“…A counts table for unambiguous uniquely mapped reads was generated using a custom R script. Coordinates for snu4 (II: 467,488–467,615) 47 and snu5 (II: 3,236,356–3,237,312) (GenBank: X16573.1) were manually curated based on alignment of the published sequences to the genome. Differential gene expression analysis was performed with EdgeR 48 (v.3.14.0) using the likelihood ratio test with the Benjamini–Hochberg false discovery rate correction.…”

Section: Methodsmentioning

confidence: 99%

Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

et al. 2018

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Telomerase reverse transcriptase (TERT) and the non-coding telomerase RNA subunit (TR) constitute the core of telomerase. Here we now report that the putative F-box protein Pof8 is also a constitutive component of active telomerase in fission yeast. Pof8 functions in a hierarchical assembly pathway by promoting the binding of the Lsm2-8 complex to telomerase RNA, which in turn promotes binding of the catalytic subunit. Loss of Pof8 reduces TER1 stability, causes a severe assembly defect, and results in critically short telomeres. Structure profile searches identified similarities between Pof8 and telomerase subunits from ciliated protozoa, making Pof8 next to TERT the most widely conserved telomerase subunits identified to date.

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Section: Methodsmentioning

confidence: 99%

Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

et al. 2018

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“…Moreover degradation of end-labelled RNA gives rise to a nested set of fragments with a distinctive diagonal pattern on two-dimensional gels. The analogues of U 1, U2, U4, U5 and U6 are strikingly well conserved between S.pombe and mammals (16,18,19,20,21) and these, together with a number of much lower abundance snRNAs are precipitated by human anti-Sm sera. Surprisingly, three anti-Sm sera tested distinguish between Ul and U2, U4 and U5.…”

Section: Discussionmentioning

confidence: 99%

Immunoprecipitation distinguishes non-overlapping groups of snRNPs inSchizosaccharomyces pombe

Tollervey¹,

Tessars

Lührmann

1990

Nucl Acids Res

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The large number of snRNAs in the fission yeast Schizosaccharomyces pombe can be divided into four non-overlapping groups by immunoprecipitation with antibodies directed against mammalian snRNP proteins. 1) Of the abundant snRNAs, anti-Sm sera precipitate only the spliceosomal snRNAs U1, U2, U4, U5 and U6. Surprisingly, three Sm-sera tested distinguish between U2, U4 and U5 and U1 from S.pombe; one precipitating only U1 and two precipitating U2, U4 and U5 but not U1. 2) A group of 11 moderately abundant snRNAs are not detectably precipitated by human anti-Sm sera, but are specifically precipitated by monoclonal antibody H57 specific for the human B/B' polypeptides. From Aspergillus nidulans this antibody also precipitates at least 12 snRNAs. 3) Anti-(U3)RNP sera do not precipitate the above snRNAs, but precipitate at least 6 further snRNAs, including the homologues of U3. Both the anti-(U3)RNP sera and H57 also efficiently precipitate a number of discrete non-capped RNAs. 4) A small number of additional snRNAs are not detectably precipitated by any anti-serum tested to date, further analysis may identify antisera specific for these snRNPs. Western blots of purified snRNP proteins were used to identify the S.pombe proteins responsible for these immunoprecipitations. Several Sm-sera decorate a 16.3kD protein which may be a D protein homologue, monoclonal H57 decorates a further protein of 16kD and an anti-(U3)RNP serum decorates the homologue of the 36kD U3-specific protein, fibrillarin.

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“…Each of the spliceosomal snRNAs from S. cerevisiae, including Ul, has been shown to be essential for viability (1,8,29,37,39,40), and recently U4 from S. pombe was likewise demonstrated to be indispensable for growth (11). It was thus not unexpected that disruption of the S. pombe Ul gene is lethal.…”

Section: Discussionmentioning

confidence: 99%

“…The Sm site in fission yeast Ul, presumably responsible for binding to the common snRNP protein core, conforms to the canonical sequence AU4_6G (5). This contrasts with U2, U4, and U5 snRNAs from this organism, which all have a G preceding the run of uridines (7,11,43).…”

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confidence: 90%

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U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene.

Porter¹,

Brennwald²,

Wise³

1990

Mol. Cell. Biol.

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We have cloned, sequenced, and disrupted the gene encoding Ul small nuclear RNA (snRNA) The nuclei of all eucaryotic cells contain a class of low-molecular-weight capped RNA species designated Uclass small nuclear RNAs (snRNAs) (for a review, see reference 12). Five of these (Ul, U2, U4, U5, and U6) are components of small nuclear ribonucleoprotein particles (snRNPs) that participate in the splicing of mRNA precursors (for a review, see reference 44). A primary function of the U snRNPs is to recognize splicing signals in the premRNA. Even before the mechanism of intron removal was understood, binding of Ul to the substrate was proposed on the basis of potential hydrogen bonding between nine nucleotides near the 5' terminus of the snRNA and mammalian 5' splice junctions. Analysis of compensatory base changes in both human (50) and Saccharomyces cerevisiae (budding yeast) cells (37,38, 40) provided direct proof of base pairing between Ul and 5' splice sites at some, but not all, positions tested. The first 10 nucleotides of Ul snRNA from S. cerevisiae and mammals are identical in spite of differences in the 5' splice site consensus sequences, suggesting that they may have function(s) in addition to substrate recognition (12). Yeast Ul is nearly four times as large as human Ul (18, 41), yet it lacks certain structures otherwise universally conserved (17).The protein components of snRNPs include a common core recognized by specific autoimmune sera, as well as a variable number of polypeptides uniquely associated with a particular snRNA (for a review, see reference 21). The recent isolation of cDNA clones for the three Ul snRNPspecific proteins has allowed mapping of their binding sites on the RNA. A variety of in vitro assays demonstrate that the 70K protein (molecular weight of 55,000) interacts specifically with stem-loop I (31, 34, 45), while the A protein binds to stem-loop II (22,30). The C protein also appears to be associated with the 5' portion of Ul (14), but some controversy exists as to its specific binding site on the RNA (30). * Corresponding author.Signals which direct the synthesis of U snRNAs have been extensively characterized in higher eucaryotes (for a review, see reference 9). On the basis of ao-amanitin sensitivity, U snRNAs are thought to be transcribed by RNA polymerase II, with the exception of U6, which is a pol III transcript. Several features distinguish their synthesis from that of mRNAs, however. Metazoan snRNA promoters lack a canonical TATA sequence; instead, the position of transcription initiation is specified by a conserved motif centered at -50 to -60 (versus -25 to -35 for mRNA TATA boxes). A distal sequence element located between -200 and -250 is similar to, but not interchangeable with, enhancers located upstream from mRNA coding sequences. Perhaps most intriguing is the 3' box located just downstream of the human Ul and U2 genes, which directs formation of the proper 3' end but is bypassed if transcription was initiated at an mRNA rather than an snRNA promoter. Little is kn...

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Schizosaccharomyces pombe U4 small nuclear RNA closely resembles vertebrate U4 and is required for growth

Cited by 17 publications

References 35 publications

Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast

Immunoprecipitation distinguishes non-overlapping groups of snRNPs inSchizosaccharomyces pombe

U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene.

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