Scenedesmus obliquus as feedstock for biohydrogen production by Enterobacter aerogenes and Clostridium butyricum

Batista, Ana Paula; Moura, Patrícia; Marques, Paula; Ortigueira, Joana; Alves, Luís Manuel; Gouveia, Luísa

doi:10.1016/j.fuel.2013.09.077

Cited by 144 publications

(39 citation statements)

References 40 publications

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“…In fact, the fermentations by C. butyricum are relatively short, evolving quickly after the initial lag phase and, generally, exhausting the sugar source in the first 6e12 h of fermentation [10]. In the first SBR batch, the lag phase was halved due to the higher percentage of initial inoculum (10% v/v) and the extent of the exponential phase of hydrogen production diminished to approximately 2 h (Fig.…”

Section: Hydrogen Production In Sbrmentioning

confidence: 99%

“…Several studies have already shown that this type of feedstock is adequate and easily convertible in a bioH 2 production setting. Scenedesmus obliquus, a microalga with the capacity to store glucose-based carbohydrates, was efficiently converted into hydrogen by Clostridium butyricum and Enterobacter aerogenes [9,10]. The production process required no biomass pre-treatment, relied solely on wet or dry microalgal biomass as a carbon and energy source and the hydrogen production yield by C. butyricum and E. aerogenes was 69% and 34% of the maximum theoretical yield from glucose (4 mol/mol), respectively.…”

Section: Introductionmentioning

confidence: 99%

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Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum

Ortigueira

Pinto

Gouveia

et al. 2015

Energy

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a b s t r a c tThe biological hydrogen production from Spirogyra sp. biomass was studied in a SBR (sequential batch reactor) equipped with a biogas collecting and storage system. Two acid hydrolysis pre-treatments (1N and 2N H 2 SO 4 ) were applied to the Spirogyra biomass and the subsequent fermentation by Clostridium butyricum DSM 10702 was compared. The 1N and 2N hydrolyzates contained 37.2 and 40.8 g/L of total sugars, respectively, and small amounts of furfural and HMF (hydroxymethylfurfural). These compounds did not inhibit the hydrogen production from crude Spirogyra hydrolyzates. The fermentation was scaled up to a batch operated bioreactor coupled with a collecting system that enabled the subsequent characterization and storage of the biogas produced. The cumulative hydrogen production was similar for both 1N and 2N hydrolyzate, but the hydrogen production rates were 438 and 288 mL/L.h, respectively, suggesting that the 1N hydrolyzate was more suitable for sequential batch fermentation. The SBR with 1N hydrolyzate was operated continuously for 13.5 h in three consecutive batches and the overall hydrogen production rate and yield reached 324 mL/L.h and 2.59 mol/mol, respectively. This corresponds to a potential daily production of 10.4 L H 2 /L Spirogyra hydrolyzate, demonstrating the excellent capability of C. butyricum to produce hydrogen from microalgal biomass.

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Section: Hydrogen Production In Sbrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum

Ortigueira

Pinto

Gouveia

et al. 2015

Energy

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“…Exhaustive studies have been done on the production of biofuels through biological and thermochemical methods using microalgae. Microalgae biomass can also be used as the feedstock for hydrogen production by dark fermentation [4][5][6]. For this process it is advantageous that the microalgae have the ability to accumulate a high content of storage carbohydrates.…”

Section: Introductionmentioning

confidence: 99%

The production of pigments & hydrogen through a Spirogyra sp. biorefinery

Pacheco

Ferreira

Pinto

et al. 2015

Energy Conversion and Management

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a b s t r a c tThis paper discusses the overall energy consumption and greenhouse gas emissions when extracting pigments and producing hydrogen from Spirogyra sp. microalga biomass. The energy evaluation from the biomass leftovers was also included in this work. The influence of the functional unit and different allocation criteria on the biorefinery assessments is also shown. The study consists of laboratory tests showing Spirogyra sp. growth, harvesting, drying, pigment extraction and fermentation by Clostridium butyricum. Electrocoagulation and solar drying were tested and compared to conventional centrifugation and electrical dewatering in terms of their energy consumption for harvesting and dewatering, respectively. To discuss the biorefinery viability, the pigments and biohydrogen (bioH 2 ) retail costs are considered against operational costs according to electricity needs. The low yield of biochemical hydrogen and the high energy requirements for the pigment extraction were identified as main topics for further research. This research hopefully contributes to highlight the importance of energy and emission balances in order to decide on feasibility of the biorefinery.

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“…Previous studies carried out by one of the authors [7,13,30], showed that the strain of the E. aerogenes used in this study was found to be more efficient in the degradation of JSC than of the other complex substrates (residual biomass) such as microalgal biomass of S. obliquus (57.6 mL H 2 /g VS alga from 2.5 g alga /L) [13], Nannochloropsis sp. (60.6 mL/g dry biomass ) [30] and biomass of the cyanobacteria Anabaena sp.…”

Section: Effect Of Thermal Pretreatmentmentioning

confidence: 73%

“…Good H 2 -producers include mesophiles, such as species of clostridia and Enterobacter, and thermophiles such as Thermotoga neapolitana [10]. Enterobacter aerogenes, an anaerobic facultative bacterium, has been described as a good H 2 producer in fermentations with the most diverse substrates, such as organic urban solid wastes [11], biodiesel residues containing glycerol [12] and cyanobacterial and microalgal biomass, such as Anabaena [7], Scenedesmus obliquus [13] and Nannochloropsis [14] biomass.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Jatropha curcas seed cake to hydrogen by a strain of Enterobacter aerogenes

Lopes

Fragoso

Duarte

et al. 2015

Fuel

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h i g h l i g h t sJatropha curcas seed cake biomass (JSC) was used as feedstock for bioH 2 production. A pure culture of the bacteria Enterobacter aerogenes fermented JSC. E. aerogenes was efficient for 2.5-10 gVS JSC /L FM with a maximum of 238.2 mL H 2 /L FM . A new pathway to enhance by-products valorization in biodiesel production chain. t r a c tHydrogen (H 2 ) gas is considered the future energy carrier as a clean fuel. Biological processes to produce hydrogen are very attractive due to less energy expenditures and the possibility to use organic wastes as substrate. In this work, Jatropha curcas L. seed cake (JSC), a solid residue remaining after oil extraction from J. curcas seeds for biodiesel production, was used as substrate in a dark fermentation process by a pure strain of the bacteria Enterobacter aerogenes. Batch assays were performed using the substrate (2.5 gVolatile Solid/L Fermentation Medium ) submitted to thermal pretreatment in an autoclave for two different exposure times (15 and 30 min) and the results were compared with the ones obtained when the JSC was used without pretreatment. The best specific biohydrogen production (68.2 mL H 2 /gVS iJSC ) was attained for the conditions of no substrate pretreatment, which is an advantage from the viewpoint of energy saving. In the best conditions, the increase of the initial JSC concentration from 2.5 to 10 gVS/ L FM led to the increase of the cumulative hydrogen production and to higher bioH 2 production rates. However a decrease on the specific H 2 production from 68.2 to 23.5 mL H 2 /gVS iJSC was observed.

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Scenedesmus obliquus as feedstock for biohydrogen production by Enterobacter aerogenes and Clostridium butyricum

Cited by 144 publications

References 40 publications

Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum

Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum

The production of pigments & hydrogen through a Spirogyra sp. biorefinery

Bioconversion of Jatropha curcas seed cake to hydrogen by a strain of Enterobacter aerogenes

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