SCCmec typing of PVL-positive community-acquired Staphylococcus aureus (CA-MRSA) at a Japanese hospital

Abstract

The epidemiology of Panton-Valentine leukocidin (PVL)-positive MRSA in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was examined. Three hundred and forty-two CA-MRSA strains that were susceptible to imipenem and cefazolin were isolated from 1107 samples (intravenous catheter, blood, sputum, urine, skin, wound, and pharynx) from outpatients at Showa University Hospital in Japan between September 2009 and March 2017. The PVL gene was detected in 46 of 342 CA-MRSA strains, accounting for 13.5%. The type of SCC mec was determined by detection of each SCC mec -specific region, class complex, and ccr . SCC mec type IV comprised 33 strains, type V comprised 5 strains, type VII comprised 4 strains, and the unclassified type comprised 4 strains. Among the type IV strains, subtype IVa was dominant, comprising 23 of 33 strains, and the remaining 10 strains were of varying subtypes. The SCC mec type III-specific region, CZ049, was amplified in 2 type V strains, 4 type VII strains, and 4 unclassified strains. In 4 unclassified strains, CZ049 and ccr5 were detected, but neither the SCC mec -specific region nor class complex was detected. The PVL-positive rate was lower than that in Western countries. The SCC mec types of PVL-positive CA-MRSA strains were found to vary, indicating a diverse spreading route.

Prevalence of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus clinical isolates at a university hospital in Northern Cyprus: a pilot study

“…Large-scale analysis of pediatric patients with invasive S. aureus infections recruited from 13 centers in 7 European countries has revealed that overall PVL positivity rate in MRSA isolates in 2016 was 7.8% [21]. On the other hand, reports show that the prevalence of pvl gene among MRSA strains in a Japanese hospital in 2019 was 13.5% [35], whereas PVL-positivity was higher at a rate of 39% among MRSA isolates collected from pediatric patients in China within the same year [36]. Epidemiological surveillance of MRSA strains collected between 2012 and 2017 in Australia showed that 28% of the isolates were PVL positive [37].…”

Verificação Da Temperatura De Distribuição De Refeições Quentes Ofertadas Em Uma Instituição De Longa Permanência Para Idosos E a Correlação Com O Crescimento Microbiológico

“…Quando em contato com as membranas celulares do hospedeiro, reduz a resistência imunológica por lise de leucócitos ou apoptose e tem como alvo os receptores de complemento (GOUDARZI et al, 2019). Em vários estudos, os isolados de S. aureus portadores da toxina PVL estão ligados a infecções cutâneas, incluindo abscessos, furúnculos e feridas cirúrgicas (CHANGCHIEN et al, 2016;FUNAKI et al, 2019;HARCH et al, 2017;IMMERGLUCK et al, 2017;OLANIYI et al, 2018;SHAHINI SHAMS ABADI et al, 2017;SINA et al, 2013). (GOUDARZI et al, 2019).…”

Staphylococcus aureus: UMA VISÃO GERAL DOS MECANISMOS DE VIRULÊNCIA E RESISTÊNCIA

“…Quando em contato com as membranas celulares do hospedeiro, reduz a resistência imunológica por lise de leucócitos ou apoptose e tem como alvo os receptores de complemento (GOUDARZI et al, 2019). Em vários estudos, os isolados de S. aureus portadores da toxina PVL estão ligados a infecções cutâneas, incluindo abscessos, furúnculos e feridas cirúrgicas (CHANGCHIEN et al, 2016;FUNAKI et al, 2019;HARCH et al, 2017;IMMERGLUCK et al, 2017;OLANIYI et al, 2018;SHAHINI SHAMS ABADI et al, 2017;SINA et al, 2013 (GOUDARZI et al, 2019). Os isolados de MRSA produtores de toxina PVL podem causar infecções mais graves e complicadas, com maior taxa de mortalidade quando comparadas às cepas que não produzem toxina PVL (ZHANG et al, 2016).…”