Scavenger receptor C promotes bacterial clearance in kuruma shrimp Marsupenaeus japonicus by enhancing hemocyte phagocytosis and AMP expression

Yang, Ming-Chong; Yang, Huiting; Li, Jing; Sun, Jiejie; Bi, Wenjie; Niu, Guo-Juan; Zhang, Qiang; Shi, Xiaoping; Zhao, Xiao‐Fan; Wang, Jin-Xing

doi:10.1016/j.fsi.2017.06.003

Cited by 22 publications

(16 citation statements)

References 36 publications

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“…As one member of SRs, SRC has only been identified in a few invertebrates, including Drosophila melanogaster, Aedes aegypti , and Marsupenaeus japonicus . Especially, previous studies only reveal the function of SRC in immunological process (Rämet et al, 2001; Yang et al, 2016, 2017), and whether SRC participates in growth regulation remains largely unknown. Indeed, although SRs family encompasses a wide range of molecules with little structural homology (Canton et al, 2013), almost all of them have been characterized in vertebrates by the common feature to bind modified low density lipoproteins (LDLs), such as oxidized LDL (OxLDL) and acetylated LDL (AcLDL).…”

Section: Discussionmentioning

confidence: 99%

A Novel Candidate Gene Associated With Body Weight in the Pacific White Shrimp Litopenaeus vannamei

Wang

Zhang

et al. 2019

Front. Genet.

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Improvements of growth traits are always the focus in selective breeding programs for the Pacific white shrimp Litopenaeus vannamei ( L. vannamei ). Identification of growth-related genes or markers can contribute to the application of modern breeding technologies, and thus accelerate the genetic improvement of growth traits. The aim of this study was to identify the genes and molecular markers associated with the growth traits of L. vannamei . A population of 200 individuals was genotyped using 2b-RAD techniques for genome-wide linkage disequilibrium (LD) analysis and genome-wide association study (GWAS). The results showed that the LD decayed fast in the studied population, which suggest that it is feasible to fine map the growth-related genes with GWAS in L. vannamei . One gene designated as LvSRC , encoding the class C scavenger receptor (SRC), was identified as a growth-related candidate gene by GWAS. Further targeted sequencing of the candidate gene in another population of 322 shrimps revealed that several non-synonymous mutations within LvSRC were significantly associated with the body weight ( P < 0.01), and the most significant marker (SRC_24) located in the candidate gene could explain 13% of phenotypic variance. The current results provide not only molecular markers for genetic improvement in L. vannamei , but also new insights for understanding the growth regulation mechanism in penaeid shrimp.

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Section: Discussionmentioning

confidence: 99%

A Novel Candidate Gene Associated With Body Weight in the Pacific White Shrimp Litopenaeus vannamei

Wang

Zhang

et al. 2019

Front. Genet.

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“…To determine whether HaCTL3 was involved in antibacterial activity, we performed in vivo bacterial clearance assay as described previously [ 71 ]. Briefly, larvae were injected with E .…”

Section: Methodsmentioning

confidence: 99%

“…To determine whether HaCTL3 was involved in antibacterial activity, we performed in vivo bacterial clearance assay as described previously [71]. Briefly, larvae were injected with E. coli PLOS PATHOGENS or S. aureus (2 × 10 7 CFUs/larva) at 96 hpi of HaCTL3 or GFP dsRNA (control).…”

Section: In Vivo Bacterial Clearance Assaymentioning

confidence: 99%

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C-type lectin-mediated microbial homeostasis is critical for Helicoverpa armigera larval growth and development

Wang

Zhuo

et al. 2020

PLoS Pathog

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The immune system of a host functions critically in shaping the composition of the microbiota, and some microbes are involved in regulating host endocrine system and development. However, whether the immune system acts on endocrine and development by shaping the composition of the microbiota remains unclear, and few molecular players or microbes involved in this process have been identified. In the current study, we found that RNA interference of a C-type lectin (HaCTL3) in the cotton bollworm Helicoverpa armigera suppresses ecdysone and juvenile hormone signaling, thus reducing larval body size and delaying pupation. Depletion of HaCTL3 also results in an increased abundance of Enterocuccus mundtii in the hemolymph, which may escape from the gut. Furthermore, HaCTL3 and its controlled antimicrobial peptides (attacin, lebocin, and gloverin) are involved in the clearance of E. mundtii from the hemolymph via phagocytosis or direct bactericidal activity. Injection of E. mundtii into larval hemocoel mimics HaCTL3-depleted phenotypes and suppresses ecdysone and juvenile hormone signaling. Taken together, we conclude that HaCTL3 maintains normal larval growth and development of H. armigera via suppressing the abundance of E. mundtii in the hemolymph. Our results provide the first evidence of an immune system acting on an endocrine system to modulate development via shaping the composition of microbiota in insect hemolymph. Thus, this study will deepen our understanding of the interaction between immunity and development.

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“…MjSRC, a class C scavenger receptor characterized from M. japonicus, was found to be able to mediate phagocytosis of WSSV by binding the viral envelope protein VP19 and then restrict viral replication (37). Meanwhile, MjSRC could also act as a phagocytotic receptor for enhancing phagocytosis and restricting bacterial proliferation through recognizing V. anguillarum and S. aureus using its extracellular domains to bind bacterial polysaccharides, such as LPS and LTA (38). Owing to abundant heterogeneous classes of the SRs, it is worthy to further prove whether any other classes of SRs, besides SR-Bs and SR-Cs, are participating in hemocyte phagocytosis in crustaceans against bacterial or viral infection.…”

Section: Scavenger Receptorsmentioning

confidence: 99%

Hemocyte-Mediated Phagocytosis in Crustaceans

Liu

Zheng

et al. 2020

Front. Immunol.

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Phagocytosis is an ancient, highly conserved process in all multicellular organisms, through which the host can protect itself against invading microorganisms and environmental particles, as well as remove self-apoptotic cells/cell debris to maintain tissue homeostasis. In crustacean, phagocytosis by hemocyte has also been well-recognized as a crucial defense mechanism for the host against infectious agents such as bacteria and viruses. In this review, we summarized the current knowledge of hemocyte-mediated phagocytosis, in particular focusing on the related receptors for recognition and internalization of pathogens as well as the downstream signal pathways and intracellular regulators involved in the process of hemocyte phagocytosis. We attempted to gain a deeper understanding of the phagocytic mechanism of different hemocytes and their contribution to the host defense immunity in crustaceans.

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Scavenger receptor C promotes bacterial clearance in kuruma shrimp Marsupenaeus japonicus by enhancing hemocyte phagocytosis and AMP expression

Cited by 22 publications

References 36 publications

A Novel Candidate Gene Associated With Body Weight in the Pacific White Shrimp Litopenaeus vannamei

A Novel Candidate Gene Associated With Body Weight in the Pacific White Shrimp Litopenaeus vannamei

C-type lectin-mediated microbial homeostasis is critical for Helicoverpa armigera larval growth and development

Hemocyte-Mediated Phagocytosis in Crustaceans

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