Scale-up of naringinase production process based on the constant oxygen transfer rate for a novel strain of Bacillus methylotrophicus

Abstract

Naringinase bioprocess based on Bacillus methylotrophicus was successfully scaled up based on constant oxygen transfer rate (OTR) as the scale-up criterion from 5-L bioreactor to 20-L bioreactor. OTR was measured in 5 and 20-L bioreactor under various operating conditions using dynamic method. The operating conditions, where complete dispersion was observed were identified. The highest OTR of 0.035 and 0.04 mMol/L/s was observed in 5 and 20-L bioreactor, respectively. Critical dissolved oxygen concentration of novel isolated strain B. methylotrophicus was found to be 20% of oxygen saturation in optimized medium. The B. methylotrophicus cells grown on sucrose had maximum oxygen uptake rate of 0.14 mMol/L/s in optimized growth medium. The cells produced the maximum naringinase activity of 751 and 778 U/L at 34 hr in 5 and 20-L bioreactors, respectively. The maximum specific growth rate of about 0.178/hr was observed at both the scales of operations. The maximum naringinase yield of 160 and 164 U/g biomass was observed in 5 and 20-L bioreactors, respectively. The growth and production profiles at both scales were similar indicating successful scale-up strategy for B. methylotrophicus culture.

VOLUMETRIC POWER INPUT AS A RELIABLE PARAMETER FOR SCALE-UP FROM SHAKE FLASK TO STIRRED-TANK BIOREACTOR: PRODUCTION OF A RECOMBINANT GLYCOPROTEIN BY Streptomyces lividans

“…In addition, for commercial production, many bioprocesses need to occur on a larger scale than in shake flasks; therefore, it is necessary to scale-up these to bioreactors, in which, it is possible to monitor and control several variables of the process independently (Tajsoleiman et al 2019). Various criteria have been proposed for scaling-up biotechnological processes, such as the volumetric mass transfer coefficient (k L a) (Humphrey, 1998;Garcia-Ochoa and Gomez, 2009;, the oxygen transfer rate (Garcia-Ochoa and Gomez, 2009;Raval et al 2017), impeller tip speed (Junker et al 2009), mixing/circulation time (Junker, 2004), and volumetric power input (P/V) (Pérez et al 2006;Rocha-Valadez et al 2006Xu et al 2017). P/V is a global parameter defined as the amount of energy required to maintain the fluid motion within a vessel for a given period of time (Marques et al 2010), and it is associated with the physical phenomena that occur in the bioreactor, such as oxygen transfer, removal of carbon dioxide, macro and micro mixing, turbulence intensity, uniformity of the liquid phase, and hydrodynamic stress (Lara 2011, Olmos et al 2013.…”