VOLUMETRIC POWER INPUT AS A RELIABLE PARAMETER FOR SCALE-UP FROM SHAKE FLASK TO STIRRED-TANK BIOREACTOR: PRODUCTION OF A RECOMBINANT GLYCOPROTEIN BY Streptomyces lividans

Abstract

Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)

Fed-batch cultivation and operational conditions for the production of a recombinant anti-amoebic vaccine in Pichia pastoris system.

“…Performing a scale-up process under a constant Pg/V, may guarantee the same k L a between scales, which ensures a successful scale-up. Also, Pg/V is a parameter well associated with the physical phenomena of mass, heat and momentum transport; and is the only scale-up criteria free of heterogeneities in dynamic bioreactors (Gamboa-Suasnavart et al, 2019). Based on these assumptions, we made the scale-up from shake flask to stirred bioreactor by coupling the Pg/V between bioreactors.…”

“…However, in the recent two decades there have been an increase in number of works to understand from an engineering point of view the process to scale-up bioprocesses from shake flasks to STB. As well for STB are used scale-up criteria to take bioprocesses from laboratory to pilot and large scale, the same occurs to transfer bioprocesses from shake flask to fermenters (Gamboa-Suasnavart et al, 2019).…”

Bioreactors for remediation of hydrocarbons in rivers and lagoons of San Luis Potosí

“…The airlift bioreactors (ALB) are a low-cost alternative used in remediation of hydrocarbons (Manowattana et al, 2018;Nápoles-Álvarez et al, 2017). Furthermore, ALB has advantages over other types of reactors such as bubble column and stirred tank: reduced cell damage, high aeration rate and more significant mass transfer (Gamboa-Suasnavart et al, 2019;Sandoval-Herazo et al, 2020). The hydrodynamics highly influences efficiency of ALB in the gas phase (evaluated with gas holdup and Sauter mean diameter) (Jamshidi & Mostoufi, 2017).…”

Partial recovery of MRJP1 protein expressed in Pichia pastoris using chromatographic techniques

“…After, when SEC coupled to IEX was performed, it was possible to observe the same fraction (∼57 kDa protein) eluting at around 38.88 mL at a conductivity of 19.65 mS/cm without the fractions observed in the chromatograms of SEC and IEX performed separately, attributed to culture media components of Pichia pastoris during fermentation. Gamboa-Suasnavart et al deduced that it is not possible to elucidate if the change in some parameters of fluids during growth in bioreactors are the result of culture media composition, biomass, or morphology (Gamboa-Suasnavart et al, 2019). In order to study the N-glycosylation pattern of MRJP1, Pichia pastoris supernatant containing MRJP1 was deglycosylated using PNGase F. MRJP1 is a glycoprotein, with glycans at the N144 and N177 positions (Mandacaru et al, 2017), including a Galβ1-3Gal NAc unit.…”