Scaffolds in tissue engineering bone and cartilage

Hutmacher, Dietmar W.

doi:10.1016/s0142-9612(00)00121-6

Cited by 4,336 publications

(2,113 citation statements)

References 56 publications

Supporting

Mentioning

1,899

Contrasting

Unclassified

Order By: Relevance

“…The Young modulus of the titanium lattice varies gradually from 2.7 GPa to 6.6 GPa, which is also in the range of the transitions from trabecular to cortical bone, which the lattice would aim to replace or repair. In consequence, according to our results, the mechanical performance of the construct is bio mimetic, as proposed in seminal papers in the field [51], and the stiffness values of the bony and chondral phases can be tuned to the desired applications, by means of controlled modifications of the computer aided designs, of the materials used, of the rate of porogen employed, among other options that promote the versatility of the proposed approach. The design parameters used for these first prototypes provide results in the expected ranges of mechanical proper ties of the biomaterials to be repaired or replaced.…”

Section: Cell Culture Process For In Vitro Validationsupporting

confidence: 52%

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

Lantada

Iniesta

García–Ruiz

2016

Materials Science and Engineering: C

View full text Add to dashboard Cite

A b s t r a c tArticular repair is a relevant and challenging area for the emerging fields of tissue engineering and biofabrication. The need of significant gradients of properties, for the promotion of osteochondral repair, has led to the develop ment of several families of composite biomaterials and scaffolds, using different effective approaches, although a perfect solution has not yet been found. In this study we present the design, modeling, rapid manufacturing and in vitro testing of a composite scaffold aimed at osteochondral repair. The presented composite scaffold stands out for having a functional gradient of density and stiffness in the bony phase, obtained in titanium by means of computer aided design combined with additive manufacture using selective laser sintering. The chondral phase is obtained by sugar leaching, using a PDMS matrix and sugar as porogen, and is joined to the bony phase during the polymerization of PDMS, therefore avoiding the use of supporting adhesives or additional intermediate layers. The mechanical performance of the construct is biomimetic and the stiffness values of the bony and chondral phases can be tuned to the desired applications, by means of controlled modifications of different parameters. A human mesenchymal stem cell (h MSC) conditioned medium (CM) is used for improving scaffold response. Cell culture results provide relevant information regarding the viability of the composite scaffolds used.

show abstract

Section: Cell Culture Process For In Vitro Validationsupporting

confidence: 52%

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

Lantada

Iniesta

García–Ruiz

2016

Materials Science and Engineering: C

View full text Add to dashboard Cite

show abstract

“…Since the thickness and microstructure of the Bioglass® coating will influence the degradation behaviour of the composite material, an even, uniform and reproducible coating along the walls of pores is required. Moreover, for cell proliferation, tissue engineering scaffolds are required to possess sufficient pore volume, as well as pores of a given, controlled size [31]. In the interior of the composite foams fabricated by EPD, however, the original interconnected porous structure of the foam became "sealed" by the Bioglass® particles, and therefore, this technique was found to be inadequate for the purpose of fabricating porous composite scaffolds, at least for the conditions investigated here.…”

Section: Materials Characterisationmentioning

confidence: 99%

Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass® for tissue engineering applications

et al. 2002

View full text Add to dashboard Cite

Bioactive and bioresorbable composite materials were fabricated using macroporous poly(DL-lactide) (PDLLA) foams coated with and impregnated by bioactive glass (Bioglass®) particles. Stable and homogeneous Bioglasss coatings on the surface of PDLLA foams as well as infiltration of Bioglass® particles throughout the porous network were achieved using a slurry-dipping technique in conjunction with pre-treatment of the foams in ethanol. The quality of the bioactive glass coatings was reproducible in terms of thickness and microstructure. Additionally, electrophoretic deposition was investigated as an alternative method for the fabrication of PDLLA foam/Bioglass® composite materials. In vitro studies in simulated body fluid (SBF) were performed to study the formation of hydroxyapatite (HA) on the surface of PDLLA/Bioglass® composites. SEM analysis showed that the HA layer thickness rapidly increased with increasing time in SBF. The high bioactivity of the PDLLA foam/Bioglasss composites indicates the potential of the materials for use as bioactive, resorbable scaffolds in bone tissue engineering.

show abstract

“…The scaffold should be designed to offer an adequate biomechanical environment for cells and newly formed tissue. One of the main objectives when designing a scaffold is to obtain a structure that diminishes the differences in its stressstrain response with respect to the neighboring tissue at the site of the defect [2]. At the same time, the scaffold must transmit appropriate mechanical signals to the cells, which then translate mechanical stimuli to stimulate extra-cellular matrix production [3].…”

Section: Introductionmentioning

confidence: 99%

An “in vitro” experimental model to predict the mechanical behavior of macroporous scaffolds implanted in articular cartilage

Vikingsson

Ferrer

Gómez-Tejedor

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

View full text Add to dashboard Cite

A model is proposed to assess mechanical behaviour of tissue engineering scaffolds and predict their performance "in vivo" during tissue regeneration. To simulate the growth of tissue inside the pores of the scaffold, the scaffold is swollen with a Poly (Vinyl alcohol) solution and subjected to repeated freezing and thawing cycles. In this way the Poly (Vinyl alcohol) becomes a gel whose stiffness increases with the number of freezing and thawing cycles. Mechanical properties of the construct immersed in water are shown to be determined, in large extent, by the water mobility constraints imposed by the gel filling the pores. This is similar to the way that water mobility determines mechanical properties of highly hydrated tissues, such as articular cartilage. As a consequence, the apparent elastic modulus of the scaffold in compression tests is much higher than those of the empty scaffold or the gel. Thus this experimental model allows assessing fatigue behaviour of the scaffolds under long-term dynamic loading in a realistic way, without recourse to animal experimentation. L. Vikingsson, et al., J Mech Behav Biomed 32 (2014) 125-131 2

show abstract

Scaffolds in tissue engineering bone and cartilage

Cited by 4,336 publications

References 56 publications

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass® for tissue engineering applications

An “in vitro” experimental model to predict the mechanical behavior of macroporous scaffolds implanted in articular cartilage

Contact Info

Product

Resources

About