Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture

Ashton, Randolph S.; Banerjee, Akhilesh; Punyani, Supriya; Schaffer, David V.; Kane, Ravi S.

doi:10.1016/j.biomaterials.2007.08.038

Cited by 189 publications

(122 citation statements)

References 40 publications

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“…However, other methods of providing drug delivery to scaffold-type systems have arisen that might provide greater flexibility in the types and quantities of drugs that can be delivered. For example, micro- (22) or nanoparticles (23) containing drugs can be dispersed throughout a scaffold (Fig. 1).…”

Section: Drug Delivery Functionalitymentioning

confidence: 99%

Polymeric Biomaterials in Tissue Engineering

2008

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Polymeric biomaterials are one of the cornerstones of tissue engineering. A wide range of materials has been used. Approaches have shown increasing sophistication over recent years employing drug delivery functionality, micropatterning, microfluidics, and other technologies. Challenges such as producing threedimensional matrixes and rendering them deliverable through minimally invasive techniques have been addressed. A major recent development is the design of biomaterials for tissue engineering matrices to achieve specific biologic effects on cells, and vice versa.

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Section: Drug Delivery Functionalitymentioning

confidence: 99%

Polymeric Biomaterials in Tissue Engineering

2008

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“…Recent studies have investigated the use of alginate as a neural stem cell (NSC) scaffold. [5][6][7] However, the effect of alginate composition on NSC function has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Purcell

Singh

Kipke

2009

Tissue Engineering Part C: Methods

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The purpose of this study was to evaluate the effects of alginate composition on the neurotrophic factor release, viability, and proliferation of encapsulated neural stem cells (NSCs), as well as on the mechanical stability of the scaffold itself. Four compositions were tested: a high guluronic acid (68%) and a high mannuronic acid (54%) content alginate, with or without a poly-L-lysine (PLL) coating layer. Enzyme-linked immunosorbent assay was used to quantify the release of brain-derived neurotrophic factor, glial-derived neurotrophic factor, and nerve growth factor from the encapsulated cells. All three factors were detected from encapsulated cells only when a high L-guluronic acid alginate without PLL was used. Additionally, capsules with this composition remained intact more frequently when exposed to solutions of low osmolarity, potentially indicating superior mechanical stability. Alginate beads with a PLL-coated, high D-mannuronic acid composition were the most prone to breakage in the osmotic pressure test, and were too fragile for histology and proliferation assays after 1 week in vitro. NSCs survived and proliferated in the three remaining alginate compositions similarly over the 21-day study course irrespective of scaffold condition. NSC-seeded alginate beads with a high L-guluronic acid, non-PLL-coated composition may be useful in the repair of injured nervous tissue, where the mechanism is the secretion of neuroprotective factors. We verify the neuroprotective effects of medium conditioned by NSCseeded alginate beads on the serum withdrawal-mediated death of PC-12 cells here.

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“…Ashton et al describe an alternative approach to design degradable alginate hydrogels based on the enzymatic degradation of alginate polymers. They incorporate poly(lactide-coglycolide) (PLGA) microspheres loaded with the enzyme alginate-lyase (PLGA-AL) into alginate hydrogels; controlling the amount of incorporated enzyme, and its rate of release from the PLGA microspheres, enables the rate of enzymatic degradation of the alginate hydrogels to be tuned [111].…”

Section: Lim Et Al Related That Their Results On Poly(l-lactic Acid)mentioning

confidence: 99%

Three-Dimensional and Biomimetic Technology in Cardiac Injury After Myocardial Infarction: Effect of Acellular Devices on Ventricular Function and Cardiac Remodelling

Chaud¹,

Alves²,

Rebelo³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Dilated cardiomyopathy (DMC) of ischemic or non-ischemic aetiology remains a lethal condition nowadays. Despite early percutaneous or medical revascularization after an acute myocardial infarct (AMI), many patients still develop DMC and severe heart failure due to cardiac remodelling. Possibility of regenerating myocardium already damaged or at least inducing a more positive cardiac remodelling with use of biodegradable scafolds has been atempted in many experimental studies, which can be cellular or acellular. In the cellular scafolds, the cells are incorporated in the structure prior to implantation of the same into the injured tissue. Acellular scafolds, in turn, are composites that use one or more biomaterials present in the extracellular matrix (ECM), such as proteoglycans non-proteoglycan polysaccharide, proteins and glycoproteins to stimulate the chemotaxis of cellular/molecular complexes as growth factors to initiate speciic regeneration. For the development of scafold, the choice of biomaterials to be used must meet speciic biological, chemical and architectural requirements like ECM of the tissue of interest. In acute myocardial infarction, treating the root of the problem by repairing injured tissue is more beneicial to the patient. Inducing more constructive forms of endogenous repair. Thus, patches of acellular scafolds capable of mimicking the epicardium and ECM should be able to atenuate both cardiac remodelling and adverse cardiac dysfunction.

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Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture

Cited by 189 publications

References 40 publications

Polymeric Biomaterials in Tissue Engineering

Polymeric Biomaterials in Tissue Engineering

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Three-Dimensional and Biomimetic Technology in Cardiac Injury After Myocardial Infarction: Effect of Acellular Devices on Ventricular Function and Cardiac Remodelling

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