SAMPLING TECHNIQUE FOR COLLECTION OF EXPIRED CO<sub>2</sub> IN STUDIES USING NATURALLY LABELLED <sup>13</sup>C IN CALVES

Chevalier, R.; Pelletier, Guillaume; Gagnon, M.

doi:10.4141/cjas84-057

Cited by 9 publications

(9 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On day 20 of each experimental period, a solution of [ 13 C]sodium bicarbonate (99 atom %; MSD Isotopes, Montreal, Quebec, Canada; 100 mM in sterile 0⋅15 M-NaCl) was infused via a jugular catheter, inserted the previous day, at a rate of 0⋅165 mmol/h for 5 h (from 09.00 to 14.00 hours) preceded by a priming dose of 0⋅24 mmol (equivalent to 1⋅4 times the hourly rate; Wolfe, 1984). Breath samples were collected through a face mask (Chevalier et al 1984): three samples were collected during the hour preceding the beginning of the infusion to determine 13 C natural abundance in CO 2 and then at 1⋅5, 2, 3, 4 and 5 h after the beginning of the infusion. The CO 2 was purified from breath samples on a vacuum line, using differential freezing with a solid CO 2 -acetone mixture and liquid N 2 (Chevalier et al 1984).…”

Section: Infusions and Samplingmentioning

confidence: 99%

“…Breath samples were collected through a face mask (Chevalier et al 1984): three samples were collected during the hour preceding the beginning of the infusion to determine 13 C natural abundance in CO 2 and then at 1⋅5, 2, 3, 4 and 5 h after the beginning of the infusion. The CO 2 was purified from breath samples on a vacuum line, using differential freezing with a solid CO 2 -acetone mixture and liquid N 2 (Chevalier et al 1984).…”

Section: Infusions and Samplingmentioning

confidence: 99%

See 1 more Smart Citation

The effect of intake on protein metabolism across splanchnic tissues in growing beef steers

et al. 1999

View full text Add to dashboard Cite

The contribution of the total splanchnic tissue (TSP; portal-drained viscera (PDV) plus liver) to whole-body protein metabolism was estimated in relation to intake (0·6, 1·0 and 1·6 × maintenance requirements), in six multicatheterized growing beef steers used in a double 3 × 3 Latin square design. At the end of each 21 d experimental period, [1-13C]leucine was infused into a jugular vein (1·05 mmol/h for 5 h, preceded by a priming dose of 1·05 mmol). Arterial, portal and hepatic blood samples were collected hourly during the infusion. The increment in TSP leucine irreversible loss rate (ILR) observed with increasing intake reached significance (P< 0·10) only for PDV, while whole-body ILR increased markedly (P< 0·001) with intake. The relative contribution of TSP to whole-body leucine ILR averaged 44 % (25 % from PDV and 19 % from the liver). Although these proportions were not affected by intake, on an incremental basis more than 70 % of the increase of whole-body leucine ILR between the 0·6 and 1·0 × maintenance originated from the changes in TSP ILR, while the corresponding value was below 13 % between 1·0 and 1·6 × maintenance. Total whole-body leucine oxidation and fractional oxidation increased (P< 0·05) with intake. Protein retention increased with intake (P< 0·01), as a result of a greater increase in protein synthesis compared with protein degradation. Protein breakdown had a major impact on protein turnover as 65 % of the protein synthesized was degraded when intake varied from 1·0 to 1·6 × maintenance. Net leucine portal absorption increased (P< 0·001) with intake and represented 1, 16 and 23 % of whole body leucine ILR, for 0·6, 1·0 and 1·6 × maintenance, respectively. Although leucine oxidation was not a major component of whole body ILR (9·3–19·9 %), it represented 69 % of the net available leucine (portal absorption) even at 1·6 × maintenance. The lower relative contribution of the TSP to whole-body leucine ILR at higher intake indicates the proportional increase in the metabolic activity of peripheral tissues as the animals moved into positive protein balance.

show abstract

Section: Infusions and Samplingmentioning

confidence: 99%

Section: Infusions and Samplingmentioning

confidence: 99%

The effect of intake on protein metabolism across splanchnic tissues in growing beef steers

et al. 1999

View full text Add to dashboard Cite

show abstract

“…Stable isotope breath samples are used in clinical medicine, particularly in the field of paediatrics, where they are used as a non‐invasive test for gastrointestinal, nutritional and metabolic processes (Weaver 1998). Similar applications are used for carbon breath tests in the field of veterinary science (for example, Chevalier et al . 1984).…”

Section: Introductionmentioning

confidence: 99%

Measuring natural abundance of ¹³C in respired CO₂: variability and implications for non‐invasive dietary analysis

Perkins

Speakman

2001

Functional Ecology

View full text Add to dashboard Cite

Summary 1.Three experiments were performed, using laboratory mice ( Mus musculus ) as a model species, to evaluate the potential of using measurements of carbon isotope ratios in expired CO 2 for tracing diets. 2. Breath 13 C signatures of mice fed a constant diet (-21·4‰ ± 0·35) reflected their diet, but were depleted by on average -5·7‰. Body mass, sex and age were independent and significant factors correlated with the variability of 13 C enrichment in respired CO 2 . 3. Breath 13 C signatures from starved mice (7 h) were lower than unstarved mice by 2·0‰. Subsequently when starved mice were fed a small meal of a new diet, breath 13 C signatures approached those of the new diets within 15 min, returning to preingestion levels after 105 min. 4. After a permanent diet switch 13 C values of breath were not asymptotic within 6 days, possibly because of use of fat reserves during the daytime carrying an isotopic memory of the previous diet. Hence, individual breath 13 C signatures may vary according to nutritive state and previous dietary history. 5. Interindividual variability was measured at 3·3‰. The implications are that large samples of individuals will be required to distinguish between diets of different populations where the isotopic difference between their diets was small -for example, that expected between herbivorous and carnivorous diets. However, breath would be suitable for distinguishing between dietary intakes of individuals for food types that are isotopically more distinct -such as between C 3 and C 4 plants.

show abstract

“…During the infusion of labeled leucine, samples of expired air were taken with a face mask (Chevalier et al, 1984) with the same schedule used for blood samples. Three samples for natural abundance of expired CO 2 were taken at 15-min intervals before the labeled leucine infusion started.…”

Section: Infusions and Sampling Proceduresmentioning

confidence: 99%

“…Three samples for natural abundance of expired CO 2 were taken at 15-min intervals before the labeled leucine infusion started. Carbon dioxide in breath samples was purified on a vacuum line with differential freezing with solid CO -acetone mixture and liquid N 2 (Chevalier et al, 1984). CO 2 production.…”

Section: Infusions and Sampling Proceduresmentioning

confidence: 99%

Variations in Mammary Protein Metabolism During the Natural Filling of the Udder with Milk over a 12-h Period Between Two Milkings: Leucine Kinetics

Thivierge

Petitclerc

Bernier

et al. 2002

Journal of Dairy Science

View full text Add to dashboard Cite

To define the temporal variations of whole body and mammary leucine kinetics over a 12-h period between two milkings, we used two groups of four Holstein cows, one in their second and the other in their third or fourth lactation. Cows were infused with L-[1-13C]leucine during the 12-h interval between two milkings. Blood was sampled every 30 min during that period from arterial and mammary sources. Timeafter milking did not affect whole body irreversible loss rate of leucine but affected whole body leucine oxidation, which broadly followed variations in arterial plasma leucine concentration. Similarly, mammary leucine irreversible loss rate and leucine used for protein synthesis were not affected by time after milking. Leucine oxidation by the mammary gland was, however, affected by time after milking. It increased by 15% from the first 2-h period to the following 4-h period and then decreased by 13% over the following 2-h period. A 21% increase in leucine oxidation was observed from 8 to 10 h after milking, and then it decreased by 26% over the last 2-h period. Protein degradation expressed as percentage of mammary leucine flux followed a similar temporal pattern. Leucine used for protein synthesis by the mammary gland was unaltered over time after milking, suggesting that the increased availability of leucine resulting from mammary protein breakdown would increase intracellular concentrations of leucine, which would have favored its catabolism. Overall, these results confirm the high metabolic activity of the mammary gland, as protein synthesis by the mammary gland averaged 43% of whole body protein synthesis.

show abstract

SAMPLING TECHNIQUE FOR COLLECTION OF EXPIRED CO₂ IN STUDIES USING NATURALLY LABELLED ¹³C IN CALVES

Cited by 9 publications

References 2 publications

The effect of intake on protein metabolism across splanchnic tissues in growing beef steers

The effect of intake on protein metabolism across splanchnic tissues in growing beef steers

Measuring natural abundance of ¹³C in respired CO₂: variability and implications for non‐invasive dietary analysis

Variations in Mammary Protein Metabolism During the Natural Filling of the Udder with Milk over a 12-h Period Between Two Milkings: Leucine Kinetics

Contact Info

Product

Resources

About

SAMPLING TECHNIQUE FOR COLLECTION OF EXPIRED CO2 IN STUDIES USING NATURALLY LABELLED 13C IN CALVES

Cited by 9 publications

References 2 publications

The effect of intake on protein metabolism across splanchnic tissues in growing beef steers

The effect of intake on protein metabolism across splanchnic tissues in growing beef steers

Measuring natural abundance of 13C in respired CO2: variability and implications for non‐invasive dietary analysis

Variations in Mammary Protein Metabolism During the Natural Filling of the Udder with Milk over a 12-h Period Between Two Milkings: Leucine Kinetics

Contact Info

Product

Resources

About

SAMPLING TECHNIQUE FOR COLLECTION OF EXPIRED CO₂ IN STUDIES USING NATURALLY LABELLED ¹³C IN CALVES

Measuring natural abundance of ¹³C in respired CO₂: variability and implications for non‐invasive dietary analysis