Sampling and handling of liver tissue

Rothuizen, J.; Desmet, Valeer; Ingh, T.S.G.A.M. van den; Twedt, David C.; Bunch, Susan E.; Washabau, Robert J.

doi:10.1016/b978-0-7020-2791-8.50006-x

Cited by 40 publications

(44 citation statements)

References 14 publications

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“…The higher proportion of livers with histologic changes in our population may reflect the proportion of dogs that have nonuniform changes throughout the liver and were therefore not accounted for in the previous study. Differences in histopathologic interpretation between pathologists is a well‐documented phenomenon and likely contributed to the differences between the prevalence of hepatic changes between studies 2. In addition, this study was performed at a referral hospital rather than first opinion practices as in the previous study.…”

Section: Discussionmentioning

confidence: 99%

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Histopathologic Variation between Liver Lobes in Dogs

Kemp

Zimmerman

Panciera

et al. 2015

Veterinary Internal Medicne

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BackgroundBiopsy of the liver evaluates a small portion of tissue, with inferences made to the entire organ. The method and number of biopsies obtained are tempered by consideration of the risks and benefits. Recommendations often include biopsy of more than one liver lobe, although the consistency of histopathology among lobes in dogs is unknown.Hypothesis/ObjectivesTo describe the distribution of histopathologic abnormalities between liver lobes. We hypothesized that discordant results would be evenly distributed among all liver lobes.AnimalsSeventy dogs undergoing necropsy.MethodsProspective study. Liver samples were obtained from all lobes. A primary diagnosis was assigned to each liver sample based on the predominant histopathologic abnormality.ResultsIn this population of dogs, biopsy of at least 2 liver lobes identified the predominant histologic abnormality in 98.6% of the cases. Ten (14%) of the dogs had ≤3 lobes in agreement and could not be assigned a predominant diagnosis. The same diagnosis was present in 6/6 lobes in 39 (56.5%) dogs, 5/6 lobes in 10 (14.5%) dogs, 4/6 lobes in 10 (14.5%) dogs, 3/6 lobes in 7 (10.1%) dogs, and 2/6 in 3 (4.3%) dogs. The number of discordant results did not differ between the liver lobes.Conclusion and Clinical ImportanceThe likelihood of obtaining a sample that is reflective of the predominant histologic abnormality in the liver is increased when multiple liver lobes are biopsied.

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Section: Discussionmentioning

confidence: 99%

“…Liver biopsy is integral in the diagnosis and management of canine liver disease 1, 2. To accurately diagnose diffuse liver disease, a biopsy specimen must reliably represent the abnormalities throughout the hepatic parenchyma.…”

mentioning

confidence: 99%

Histopathologic Variation between Liver Lobes in Dogs

Kemp

Zimmerman

Panciera

et al. 2015

Veterinary Internal Medicne

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“…To facilitate client compliance, postprandial BA concentrations were not measured in this study, because inclusion of postprandial BAs would have required an additional fasting period after the measurement. Liver biopsy specimens were obtained from all dogs by the Menghini technique described by Rothuizen et al 7 Dogs were positioned in right lateral recumbency, hair was clipped around the area of the xyphoid, and the skin was aseptically prepared. A stab incision was made on the midline, 1.5 cm behind the xyphoid, after local anesthesia with 2% xylocaine, and a Menghini needle was introduced into the abdomen for retrieval of biopsy specimens.…”

Section: Methodsmentioning

confidence: 99%

Dietary Management of Hepatic Copper Accumulation in Labrador Retrievers

Hoffmann

Jones²,

Biourge

et al. 2009

Veterinary Internal Medicne

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Background: Copper-associated chronic hepatitis (CACH) recently has been recognized in the Labrador Retriever as an inherited disorder with a late onset of clinical signs. No studies have investigated dietary management for the long-term treatment of this disease or for its potential in delaying the onset of clinical signs in subclinical cases.Objectives: To investigate the effects of a low-copper diet and zinc gluconate on hepatic copper concentrations in Labrador Retrievers with abnormal hepatic copper concentrations.Animals: Twenty-four client-owned Labradors that were related to patients affected with CACH and that had been diagnosed with increased hepatic copper concentrations.Methods: Hepatic copper concentrations were assessed before and after an average of 8 and 16 months of treatment. During this time, all dogs were fed exclusively a low-copper diet. In addition, dogs were assigned to 1 of 2 groups in a randomized double-blind manner to receive a supplement of zinc gluconate or placebo.Results: Twenty-one dogs completed the study. Hepatic copper concentrations decreased in both groups at recheck 1 (n 5 21; group 1, P o .001; group 2, P 5 .001) and at recheck 2 (n5 16; group 1, P 5 .03; group 2, P 5 .04). No difference in hepatic copper concentrations was found between the 2 groups before treatment (P 5 .65), at recheck 1 or at recheck 2 (P 5 .52-.79).Conclusions and Clinical Relevance: Feeding low-copper diets to Labradors is effective in decreasing hepatic copper concentrations. Adjunctive treatment with zinc does not appear to increase the copper-lowering effects of dietary management.

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“…In 2006, the World Small Animal Veterinary Association (WSAVA) Liver Standardization Group proposed criteria for histological diagnosis of canine and feline hepatic diseases in order to unite interpretations by veterinary pathologists. After the WSAVA proposal, some reports [3, 25, 28] included retrospective reviews on diagnoses of canine liver diseases, in particular, hepatitis. Epidemiological reports on feline liver disease have never been made even after the proposal.…”

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confidence: 99%

A Retrospective Histopathological Survey on Canine and Feline Liver Diseases at the University of Tokyo between 2006 and 2012

et al. 2014

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To determine the incidence of hepatic diseases in dogs and cats in Japan, a retrospective study was performed using data of 463 canine and 71 feline liver biopsies at the Veterinary Medical Center of the University of Tokyo. The most common canine hepatic disease was microvascular dysplasia (MVD) and occupied 29.4% of all diagnoses. This terminology might contain “real” MVD and primary portal vein hypoplasia, because these two conditions were difficult to be clearly distinguished histopathologically. Parenchymal and interstitial hepatitis and primary hepatic tumors accounted for 23.5% and 21.0% of the diagnoses, respectively. Parenchymal and interstitial hepatitis occupied 34.1% of non-proliferative canine hepatic diseases, while hepatocellular adenoma and carcinoma were 26.6% and 24.5% of proliferative hepatic diseases, respectively. Breed-specificity was seen in MVD for Yorkshire terrier, Papillon and Toy poodle, in hepatitis for Doberman pinscher and Labrador retriever, in cholangiohepatitis for American cocker spaniel, Miniature schnauzer and Pomeranian, in hepatocellular adenoma for Golden retriever and Shiba and in hepatocellular carcinoma for Shih Tzu. The most common feline liver disease was parenchymal and interstitial hepatitis (45.1% of all diagnoses). Among feline hepatitis, neutrophilic cholangiohepatitis (23.9%), lymphocytic cholangiohepatitis (14.1%) and chronic hepatitis (5.6%) were recorded. Adult polycystic liver disease was 5.6%. Among proliferative diseases in the feline liver (11.3% of the all), lymphoma (4.2%) and primary epithelial tumors (4.2%) including hepatocellular carcinoma, cholangiocellular adenoma and cholangiocellular carcinoma were observed. Hepatic degeneration was 14.1%, and MVD was 12.7%, respectively.

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Sampling and handling of liver tissue

Cited by 40 publications

References 14 publications

Histopathologic Variation between Liver Lobes in Dogs

Histopathologic Variation between Liver Lobes in Dogs

Dietary Management of Hepatic Copper Accumulation in Labrador Retrievers

A Retrospective Histopathological Survey on Canine and Feline Liver Diseases at the University of Tokyo between 2006 and 2012

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