Salting-out gradients in centrifugal partition chromatography for the isolation of chlorogenic acids from green coffee beans

Romero-González, Roman R.; Verpoorte, Robert

doi:10.1016/j.chroma.2009.02.007

Cited by 44 publications

(27 citation statements)

References 35 publications

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“…Generally speaking, the organic/saltcontaining aqueous two-phase system is of low-cost and its principle is attributed to salting-out, a very common but not simple physical phenomenon extensively applied by partition study of protein, amino acid, and hydrophilic natural products [19]. Clearly, these biphasic systems composed of organic solvent and inorganic salt solution have the advantages of higher polarity compared with the conventional organic/aqueous systems.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, one kind of promising solvent system, the one-component organic/salt-containing aqueous two-phase system, has been presented to separate several natural products [19][20][21]. Generally speaking, the organic/saltcontaining aqueous two-phase system is of low-cost and its principle is attributed to salting-out, a very common but not simple physical phenomenon extensively applied by partition study of protein, amino acid, and hydrophilic natural products [19].…”

Section: Introductionmentioning

confidence: 99%

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Separation and purification of isorhamnetin 3‐sulphate from Flaveria bidentis (L.) Kuntze by counter‐current chromatography comparing two kinds of solvent systems

Yin

Zhang

Wei

2011

J of Separation Science

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The first preparative separation of a flavonoid sulphate isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze by counter-current chromatography (CCC) was presented. Two kinds of solvent systems were used. A conventional organic/aqueous solvent system n-butanol-ethyl acetate-water (4:1:5, v/v) was used, yielding isorhamnetin 3-sulphate 2.0 mg with a purity of 93.4% from 83 mg of pre-enriched crude extract obtained from 553 mg ethanol extract by macroporous resin. A one-component organic/salt-containing system composed of n-butanol-0.25% sodium chloride aqueous solution (1:1, v/v) was also used, and the LC column packed with macroporous resin has been employed for desalination of the target compound purified from CCC. As a result, 2.1 mg of isorhamnetin 3-sulphate with a purity of over 97% has been isolated from 402 mg of crude extract without pre-enrichment. Compared with the conventional organic/aqueous system, the one-component organic/salt-containing aqueous system was more suitable for the separation of isorhamnetin 3-sulphate, and purer target compound was obtained from the crude extract without pre-enrichment using the new solvent system. The chemical structure was confirmed by ESI-MS and (1)H, (13)C NMR. In summary, our results indicated that CCC using one-component organic/salt-containing aqueous solution is very promising and powerful for high-throughput purification of isorhamnetin 3-sulphate from Flaveria bidentis (L.) Kuntze.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Separation and purification of isorhamnetin 3‐sulphate from Flaveria bidentis (L.) Kuntze by counter‐current chromatography comparing two kinds of solvent systems

Yin

Zhang

Wei

2011

J of Separation Science

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“…In addition to the conventional multi-component organic/aqueous (MCO/Water) solvent systems, such as hexane-ethyl acetate-methanol-water (HEMWat), chloroform-methanol-water (ChMWat), and ethyl acetate-n-butanol-water (EBWat), a promising organic/salt polar two-phase system [10,11] have been presented to separate several natural products such as flavones. These biphasic systems had the advantages of higher polarity compared with conventional organic/aqueous systems and lower cost compared with aqueous polymer two-phase systems, as well as the lower environmental toxicity, and its principle could be attributed to salting-out, a very common but not simple physical phenomenon extensively exploited by biopolymer science, ion-exchange chromatography and counter-current separations.…”

Section: Introductionmentioning

confidence: 99%

Online polar two phase countercurrent chromatography×high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step

Chen

et al. 2015

Journal of Chromatography B

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“…Recently, great efforts have been made for the development of novel and efficient gradient CCC protocols, such as pH‐related gradient CCC, salting‐out gradient CCC, step‐gradient CCC, as well as linear gradient CCC . Wu et al developed a theoretical modeling approach to validate the feasibility of linear gradient elution in CCC .…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of bioactive constitutes from Zanthoxylum planispinum using linear gradient counter‐current chromatography

Zhang

Cheng

2015

J of Separation Science

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Counter-current chromatography is a chromatographic technique with a support-free liquid stationary phase. In the present study, a successful application of linear gradient counter-current chromatographic method for preparative isolation of bioactive components from the crude ethanol extract of Zanthoxylum planispinum was presented. The application of n-hexane/ethyl acetate/methanol/water quaternary solvents, in terms of "HEMWat" or "Arizona" solvent families, in gradient elution mode was evaluated. Results indicated that slightly proportional changes of biphasic liquid systems provided the possibility of gradient elution in counter-current chromatography, maintaining stationary phase retention in the column. With the selected quaternary solvent systems composed of n-hexane/ethyl acetate/methanol/water (2:1:2:1 and 3:2:3:2, v/v), and optimized gradient programs, in total seven fractions were separated in 4.5 h. Most of the purified compounds could be obtained at the milligram level with over 80% purity. The present study indicated that the linear gradient counter-current chromatographic approach possessed unique advantages in terms of separation efficiency, exhibiting great potential for the comprehensive separation of complex natural extracts.

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Salting-out gradients in centrifugal partition chromatography for the isolation of chlorogenic acids from green coffee beans

Cited by 44 publications

References 35 publications

Separation and purification of isorhamnetin 3‐sulphate from Flaveria bidentis (L.) Kuntze by counter‐current chromatography comparing two kinds of solvent systems

Separation and purification of isorhamnetin 3‐sulphate from Flaveria bidentis (L.) Kuntze by counter‐current chromatography comparing two kinds of solvent systems

Online polar two phase countercurrent chromatography×high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step

Preparative separation of bioactive constitutes from Zanthoxylum planispinum using linear gradient counter‐current chromatography

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