Online polar two phase countercurrent chromatography×high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step

Chen, Weibin; Li, Shu-Qi; Chen, Long-Jiang; Fang, Meijuan; Chen, Quancheng; Wu, Zhixian; Wu, Yue; Qiu, Ying-Kun

doi:10.1016/j.jchromb.2015.06.011

Cited by 20 publications

(8 citation statements)

References 22 publications

(20 reference statements)

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“…This interface allows the complete elimination of the 1 D mobile phase, the focusing of the solutes on the trapping columns and the transfer to HPLC with compatible injection solvent. Four publications have been published in 2014 and 2015 using CCC devices and preparative LC [65][66][67][68]. The interface of the first application of Qiu's group in 2014 [65] is presented in Figure 11.…”

Section: -Sample Loop Interfacementioning

confidence: 99%

“…This strategy was also applied in 2015 with an instrumental improvement on the water addition before trapping, through the use of a fragmentary dilution and turbulent mixing interface [68]. In 2014 and 2015, Qiu's group illustrated their development through two new 2D LLC-HPLC separation examples [66,67]. For these applications, the same instrumentation was enforced with the same two trapping columns.…”

Section: -Sample Loop Interfacementioning

confidence: 99%

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Preparative two dimensional separations involving liquid–liquid chromatography

Marlot

Faure

2017

Journal of Chromatography A

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The increasing performances of analytical techniques, especially two-dimensional liquid chromatography hyphenated with highly sensitive detectors, allow discovery of new targeted compounds in highly complex samples, whether biomarkers in environmental effluents, natural metabolomes in natural products or unknown impurities in synthetic chemical process. While structural identification can usually be achieved through mass spectrometry and databases, it can be useful to confirm the potential structure via NMR analysis and/or to obtain standard reference material for quantitative purposes, incentive for the production of μg to mg of new target compounds. Hence, preparative chromatography, which was initially run for large-scale production, is now facing new challenges, with small volumes of highly complex samples to deal with. Unfortunately, one dimensional chromatographic methods exhibit limited performances when targets are minor compounds in a complex matrix. Moreover, for process intensification or limited amount of sample, it is now a priority to be able to isolate multiple components with the largest yield possible using as few purification steps as possible. With these specifications in mind, a comprehensive multidimensional chromatographic method for preparative purposes is becoming appealing.

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Section: -Sample Loop Interfacementioning

confidence: 99%

Section: -Sample Loop Interfacementioning

confidence: 99%

Preparative two dimensional separations involving liquid–liquid chromatography

Marlot

Faure

2017

Journal of Chromatography A

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“…Compared with 1D separation, 2D separation usually has stronger resolving power in isolating high purity targets which may present in small amounts in samples for the combination of two techniques with different separation mechanisms. Given the circumstances, a growing interest in employing 2D preparative separation to obtain some rare components of interest from a highly complex matrix and to isolate compounds with similar chemical structures from each other has been demonstrated by many papers [1][2][3][4]. 2D-LC is the most commonly used preparative multidimensional method, and abundant applications have been investigated in both heart-cutting and comprehensive modes [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

In silico screening of off‐line comprehensive two‐dimensional counter‐current chromatography with liquid chromatography for four saponins isolation

Sun

Zhang

Bao

et al. 2022

J of Separation Science

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Being restrained by the limited peak capacity, one‐dimensional chromatography usually leads to an unsatisfactory separation with low purity of compounds in a complex mixture. To obtain more highly pure targets for standard reference and to discover new substances for structural elucidation, two‐dimensional chromatography is more and more prevalent in many fields. As few metrics on assessment of the preparative capability of two‐dimensional chromatographic separations are reported, a methodology of in silico screening of various two‐dimensional chromatographic separations with a minimal number of experiments was demonstrated in this work, which was based on three descriptors including the occupation rate of peaks and system homogeneity of a two‐dimensional separation space, and the minimal distance of all nearest‐neighbor distances of peaks. Combining the advantages of counter‐current chromatography and liquid chromatography, we elaborated the methodology by employing off‐line comprehensive two‐dimensional counter‐current chromatography with liquid chromatography to be in silico screened for separation of four saponins from Panax notoginseng at an analytical scale to simulate the case of preparative scale transfer. The predictive results were presented by two‐dimensional contour plots and verified by experiments. The result showed that the experimental results were in general accord with the predictive results.

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“…Currently, various approaches for the separation and purification of the bioactive substances have been developed, mainly including solvent extraction [8,9], ultrasound extraction [10], column chromatography method [11], counter-current chromatography method [12], and preparative high-performance liquid chromatography (prep-HPLC) method [13]. However, it is difficult to attain purified products only by solvent extraction or ultrasound extraction.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Synthetic Amanitin Epitope Imprinted Polymers via Thiol-ene Click Reaction for Recognition and Extraction α- and β-Amanitins from Mushrooms

Tan

Chen

et al. 2019

Chromatographia

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Extraction and purification of α-and β-amanitins from mushroom samples are still challenges. Surface-imprinted core-shell microspheres were synthesized combining target recognition determinant imprinting and thiol-ene click reaction, and applied to the extraction of α-and β-amanitins in mushroom samples. The highly efficient thiol-ene click reaction method offers a simple condition during the crosslinking process of molecularly imprinted polymers (MIP), and the functional monomer using could be successfully introduced onto the silica microspheres. The synthesized SiO 2 @MIP were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and thermogravimetric analysis (TGA). The measurement of SiO 2 @MIP resultants using high-performance liquid chromatography (HPLC) clearly demonstrates that SiO 2 @MIP not only display excellent affinity, but also show good selective recognition to α-and β-amanitins. The imprinted microspheres were successfully applied as sorbents for the extraction of α-and β-amanitins from mushrooms, attaining recoveries of 76.8-79.2%. Furthermore, the samples were effectively purified according to the chromatogram.

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Online polar two phase countercurrent chromatography×high performance liquid chromatography for preparative isolation of polar polyphenols from tea extract in a single step

Cited by 20 publications

References 22 publications

Preparative two dimensional separations involving liquid–liquid chromatography

Preparative two dimensional separations involving liquid–liquid chromatography

In silico screening of off‐line comprehensive two‐dimensional counter‐current chromatography with liquid chromatography for four saponins isolation

Preparation of Synthetic Amanitin Epitope Imprinted Polymers via Thiol-ene Click Reaction for Recognition and Extraction α- and β-Amanitins from Mushrooms

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