Preparative separation of bioactive constitutes from <i>Zanthoxylum planispinum</i> using linear gradient counter‐current chromatography

Zhang, Yanping; Cheng, Zhuo; Lu, Yanbin

doi:10.1002/jssc.201500489

Cited by 6 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthetic salubrinal ameliorates periodontitis by reducing alveolar bone loss and inflammatory cytokines and induces bone formation in the periodontitis-induced mouse model [ 150 ]. Zanthoxylum piperitum DC (ZP) was initially used as a food condiment and exhibits therapeutic effects including the prevention of toothache [ 151 ]. Topical treatment with ZP dried fruit extract inhibited osteoclast function and alveolar bone loss due to periodontitis in an animal study.…”

Section: Periodontitis: Treatmentmentioning

confidence: 99%

Osteoimmunology in Periodontitis: Local Proteins and Compounds to Alleviate Periodontitis

Sirisereephap

Maekawa

Tamura

et al. 2022

IJMS

View full text Add to dashboard Cite

Periodontitis is one of the most common oral diseases resulting in gingival inflammation and tooth loss. Growing evidence indicates that it results from dysbiosis of the oral microbiome, which interferes with the host immune system, leading to bone destruction. Immune cells activate periodontal ligament cells to express the receptor activator of nuclear factor kappa-B (NF-κB) ligand (RANKL) and promote osteoclast activity. Osteocytes have active roles in periodontitis progression in the bone matrix. Local proteins are involved in bone regeneration through functional immunological plasticity. Here, we discuss the current knowledge of cellular and molecular mechanisms in periodontitis, the roles of local proteins, and promising synthetic compounds generating a periodontal regeneration effect. It is anticipated that this may lead to a better perception of periodontitis pathophysiology.

show abstract

Section: Periodontitis: Treatmentmentioning

confidence: 99%

Osteoimmunology in Periodontitis: Local Proteins and Compounds to Alleviate Periodontitis

Sirisereephap

Maekawa

Tamura

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…However, it is accompanied by the problem of tedious sample preparation and diseconomy. Although high-speed counter-current chromatography (HSCCC) [ 17 , 18 ] has a high recovery rate and milligram quantity and is easy to operate for the separation of hydroxyl-α-sanshool, the main disadvantages of this method are that it is time-consuming, has low resolution, and requires large volumes of solvents. Furthermore, amides isolated from Zanthoxylum by supercritical CO 2 are in high yield [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Separation and Purification of Hydroxyl-α-Sanshool from Zanthoxylum armatum DC. by Silica Gel Column Chromatography

Cheng

Hou

Cui

et al. 2023

IJMS

View full text Add to dashboard Cite

Hydroxyl-α-sanshool is the main alkylamide produced by Zanthoxylum armatum DC., and it is responsible for numbness after consuming Z. armatum-flavored dishes or food products. The present study deals with the isolation, enrichment, and purification of hydroxyl-α-sanshool. The results indicated that the powder of Z. armatum was extracted with 70% ethanol and then filtrated; the supernatant was concentrated to get pasty residue. Petroleum ether (60–90 °C) and ethyl acetate at a 3:2 ratio, with an Rf value of 0.23, were chosen as the eluent. Petroleum ether extract (PEE) and ethyl acetate–petroleum ether extract (E-PEE) were used as the suitable enriched method. Afterward, the PEE and E-PEE were loaded onto silica gel for silica gel column chromatography. Preliminary identification was carried out by TLC and UV. The fractions containing mainly hydroxyl-α-sanshool were pooled and dried by rotary evaporation. Lastly, all of the samples were determined by HPLC. The yield and recovery rates of hydroxyl-α-sanshool in the p-E-PEE were 12.42% and 121.65%, respectively, and the purity was 98.34%. Additionally, compared with E-PEE, the purity of hydroxyl-α-sanshool in the purification of E-PEE (p-E-PEE) increased by 88.30%. In summary, this study provides a simple, rapid, economical, and effective approach to the separation of high-purity hydroxyl-α-sanshool.

show abstract

“…Some reports also demonstrate betacyanins as natural compounds for dye-sensitized solar cells [18,19]. Considerable interest led to the increasing application of CCC technique regarded as effective separation tool for complex mixtures, such as plant extracts [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…In traditional LC, the composition of the columns is restricted by producers of the columns. In addition, elimination of solid support in CCC enables to recover all components after separation as a consequence of elution extrusion process [20,23,24,[35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Separation of betacyanins from flowers of Amaranthus cruentus L. in a polar solvent system by high‐speed counter‐current chromatography

Spórna‐Kucab

Kumorkiewicz

Szmyr

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

Betacyanin extract of Amaranthus cruentus L. flowers was fractionated by semi‐preparative high‐speed counter‐current chromatography in a highly polar solvent system: propan‐1‐ol/acetonitrile/(NH4)2SO4satd. soln/H2O (1.0:0.5:1.2:1.0, v/v/v/v) in tail‐to‐head mode with 76% retention of the stationary phase. The crude extract as well as the fractions containing betacyanins were analyzed by liquid chromatography with tandem mass spectrometry as well as by high‐resolution ion‐trap time‐of‐flight mass spectrometry detection technique for the molecular formulae and multi‐step fragmentation pattern elucidation. Four betacyanins; namely, amaranthin, betanin, 6′‐O‐formyl‐amaranthin, and 6′‐O‐malonyl‐amaranthin as well as their diastereomeric forms differing in the configuration of the C‐15 carbon atom were identified in the fractions. Amaranthin was the dominant pigment in the extract and was additionally analyzed by nuclear magnetic resonance correlation techniques after the counter‐current chromatographic and high‐performance liquid chromatographic isolation. Betacyanins were highly enriched during a single high‐speed counter‐current chromatographic step; therefore, the tentative identification of new compounds for the whole Amaranthaceae family, 6′‐O‐formyl‐amaranthin and 6′‐O‐malonyl‐amaranthin was possible. Different elution profiles of the pigments observed in the counter‐current chromatographic system in comparison to high‐performance liquid chromatography system confirm a complementarity of both the techniques especially in the separation of diastereomeric pairs of betacyanins.

show abstract

Preparative separation of bioactive constitutes from Zanthoxylum planispinum using linear gradient counter‐current chromatography

Cited by 6 publications

References 29 publications

Osteoimmunology in Periodontitis: Local Proteins and Compounds to Alleviate Periodontitis

Osteoimmunology in Periodontitis: Local Proteins and Compounds to Alleviate Periodontitis

Separation and Purification of Hydroxyl-α-Sanshool from Zanthoxylum armatum DC. by Silica Gel Column Chromatography

Separation of betacyanins from flowers of Amaranthus cruentus L. in a polar solvent system by high‐speed counter‐current chromatography

Contact Info

Product

Resources

About