Salmon calcitonin‐loaded <scp>PLGA</scp> microspheres/calcium phosphate cement composites for osteoblast proliferation

Wu, Ruixue; Ma, Bin; Zhou, Quan; Tang, Cui

doi:10.1002/app.45486

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…In order to obtain such a composite, microspheres with PLGA were loaded sCT and then incorporated into CPC. The hormone’s release rate depended on the amount of the polymer phase [ 177 ]. A ceramic mixture containing 70% HAp and 30% β-TCP was modified with calcitonin by immersion in a hormone solution.…”

Section: Non-protein Biomoleculesmentioning

confidence: 99%

Polymeric and Composite Carriers of Protein and Non-Protein Biomolecules for Application in Bone Tissue Engineering

et al. 2023

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Conventional intake of drugs and active substances is most often based on oral intake of an appropriate dose to achieve the desired effect in the affected area or source of pain. In this case, controlling their distribution in the body is difficult, as the substance also reaches other tissues. This phenomenon results in the occurrence of side effects and the need to increase the concentration of the therapeutic substance to ensure it has the desired effect. The scientific field of tissue engineering proposes a solution to this problem, which creates the possibility of designing intelligent systems for delivering active substances precisely to the site of disease conversion. The following review discusses significant current research strategies as well as examples of polymeric and composite carriers for protein and non-protein biomolecules designed for bone tissue regeneration.

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Section: Non-protein Biomoleculesmentioning

confidence: 99%

Polymeric and Composite Carriers of Protein and Non-Protein Biomolecules for Application in Bone Tissue Engineering

et al. 2023

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“…3 CPC is prepared primarily by mixing the solid and liquid calcium phosphate phases with substances that can improve its properties. [4][5][6] The mixed CPC paste usually hardens within 15-30 min at 25°C, forming a solid with a certain strength. 7,8 CPC has weak mechanical properties and cannot be used for the repair of load-bearing bone tissues.…”

Section: Introductionmentioning

confidence: 99%

Novel bone cement based on calcium phosphate composited CNT curcumin with improved strength and antitumor properties

Zhao,

Zhang,

et al. 2023

Proc Inst Mech Eng H

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In this study, carboxylated carbon nanotube (CNT)-loaded curcumin (CUR) was blended into calcium phosphate cement (CPC) owing to the poor mechanical properties and single function of CPC as a bone-filling material, and CNT-CUR-CPC with improved strength and antitumor properties was obtained. The failure strength, hydrophilicity, in vitro bioactivity, bacteriostatic activity, antitumor activity, and cell safety of CNT-CUR-CPC were evaluated. The experimental results indicated that the failure strength of CNT-CUR-CPC increased from 25.05 to 45.05 MPa ( p < 0.001) and its contact angle decreased from 20.37° to 15.27° ( p < 0.001) after the CNT-CUR complex was added into CPC at the rate of 5 wt% and blended. Following soaking in simulated body fluid (m-SBF), the main components of CNT-CUR-CPC were hydroxyapatite (HA) and carbonate hydroxyapatite (HCA). The incorporation of CNT-CUR was beneficial for the deposition of PO43− and CO32−, and it promoted the crystallization of HA and HCA. For CNT-CUR-CPC, the inhibition zone diameter on Staphylococcus aureus was 10.2 ± 1.02 mm ( p < 0.001) and it exhibited moderate sensitivity, whereas the inhibition zone diameter on Escherichia coli was 8.3 ± 0.23 mm ( p < 0.001) and it exhibited low sensitivity. When compared with the CPC, the cell proliferation rate (RGR %) of the CNT-CUR-CPC decreased by 7.73% ( p > 0.05) at 24 h, 17.89% ( p < 0.05) at 48 h, and 24.43% ( p < 0.001) at 72 h when MG63 cells were cultured on it. In particular, after the MG63 cells were cultured with the CNT-CUR-CPC for 48 h, the number of newly proliferating MG63 cells was significantly reduced, and their growth and adhesion on the surface of the CNT-CUR-CPC were inhibited when compared with the CPC. When 3T3-E1 cells were exposed to the m-SBF immersion solution of CNT-CUR-CPC, the cell proliferation rate (RGR %) was ≥80% ( p > 0.05) and the cytotoxicity grade was 0–1. The 3T3-E1 cells were cultured with the m-SBF soaking solution of CNT-CUR-CPC for 24 h, and no significant changes in cell morphology or cytotoxicity were observed. After the 3T3-E1 cells were cultured on CNT-CUR-CPC for 48 h, they could stick to and grow on its surface without adverse reactions. CNT-CUR-CPC had a hemolysis rate of 4.3% ( p > 0.05) and did not result in hemolysis and hemagglutination. The obtained CNT-CUR-CPC scaffold material exhibited effective antibacterial activity and cell safety, and could achieve a certain antitumor effect, which has a wide application potential in bone tissue engineering.

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“…Polymeric scaffolds have various appropriate properties such as biocompatibility, biodegradability with proportional rate to the growth of natural tissue, also controllable mechanical properties and lifetime. Polyglycolic acid, polylactic acid, polyurethane (PU), and so forth are the most widely used synthetic polymers in the fabrication of scaffolds for biomedical applications . Herein, PU scaffolds have been attracting a large number of scientists due to biocompatibility, excellent mechanical performance, and flexibility .…”

Section: Introductionmentioning

confidence: 99%

Bioinspired polydopamine coating‐assisted electrospun polyurethane‐graphene oxide nanofibers for bone tissue engineering application

Ghorbani

Zamanian

Aidun

2019

J of Applied Polymer Sci

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It is inevitable to replace the tissues and organs that have been malfunctioning due to trauma or difference diseases, so tissue engineering can be a promising approach in case of regeneration. In this investigation, electrospun polyurethanegraphene oxide scaffold was coated with polydopamine (PDA) by immersing the constructs in dopamine hydrochloride solution under the alkaline condition for 24 h. The present study aimed to investigate the synergic effects of graphene oxide (GO) and PDA on the osteogenic expression. Field emission scanning electron microscopy, Fourier transform infrared spectrum, and X-ray diffraction (XRD) proved the synthesis of GO. Water drop contact angle and swelling absorption test indicated significantly enhancement in hydrophilicity after coating the scaffolds with PDA. Besides, PDA induced more ability of mineralization bone-like component. According to the results, cell attachment and proliferation were greatly increased in coated constructs. Alkaline phosphatase expression was also increased on the PDA-deposited polymeric scaffolds. In general, these characteristics suggest that the PDA-coated polyurethane-GO scaffolds are an appropriate substrate for in vivo studies and the bone regeneration.

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Salmon calcitonin‐loaded PLGA microspheres/calcium phosphate cement composites for osteoblast proliferation

Cited by 8 publications

References 24 publications

Polymeric and Composite Carriers of Protein and Non-Protein Biomolecules for Application in Bone Tissue Engineering

Polymeric and Composite Carriers of Protein and Non-Protein Biomolecules for Application in Bone Tissue Engineering

Novel bone cement based on calcium phosphate composited CNT curcumin with improved strength and antitumor properties

Bioinspired polydopamine coating‐assisted electrospun polyurethane‐graphene oxide nanofibers for bone tissue engineering application

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