Salinactinospora qingdaonensis gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond

Abstract

Salinactinospora qingdaonensis gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond A novel halophilic, filamentous, actinomycete strain, designated CXB832 T , was isolated from a salt pond in Qingdao, China. Optimal growth occurred at 37 6C, pH 7.0-8.0 and 9-12 % (w/v) NaCl. Strain CXB832 T formed pale yellow to deep yellow branched substrate mycelium without fragmentation. Abundant white aerial mycelium differentiated into long chains of spores and the spores were rod-shaped with smooth surfaces. Strain CXB832 T contained meso-diaminopimelic acid as the diagnostic diamino acid of the cell-wall peptidoglycan, and glucose and xylose as the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipids, glycolipid and unidentified lipids. MK-10(H 8 ), MK-9(H 8 ), MK-10(H 2 ) and MK-10(H 6 ) were the predominant menaquinones. The major fatty acids were i-C 16 : 0 (30.71 %), ai-C 17 : 0 (13.31 %) and C 16 : 0 (11.28 %). The G+C content of the DNA was 60.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family Nocardiopsaceae, but formed a separate lineage. The highest sequence similarities were to Nocardiopsis arabia DSM 45083 T (95.4 %) and Haloactinospora alba DSM 45015 T (94.9 %). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB832 T represents a new genus and novel species in the family Nocardiopsaceae, for which the name Salinactinospora qingdaonensis gen. nov., sp. nov. is proposed. The type strain of the type species is CXB832 T (5DSM 45442 T 5LMG 25567 T ).

Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil

“…The fatty acid profile contained iso-C 16 : 0 (54.14 %), C 16 : 0 (14.02 %), anteiso-C 17 : 0 (8.44 %), C 18 : 0 (5.42 %), C 18 : 1 ω7 c (5.15 %), iso-C 18 : 0 (4.17 %), 10-methyl C 18 : 0 (3.73 %), C 18 : 1 ω9 c (1.93 %), iso-C 17 : 0 (1.58 %) and anteiso-C 15 : 0 (1.41 %) (Table S1). The major components of the menaquinone, polar lipid and fatty acid profiles of Salinactinospora qingdaonensis CXB832 T were similar to those reported previously (Chang et al , 2012). The slight differences in the proportions of menaquinones and fatty acids and types of polar lipids may be due to the different experimental conditions used.…”

“…The family Nocardiopsaceae , with Nocardiopsis as the type genus, was proposed by Rainey et al (1996) based on polyphasic analysis. At the time of preparing this manuscript, the family Nocardiopsaceae contained eight genera: Nocardiopsis (Meyer, 1976), Thermobifida (Zhang et al , 1998), Streptomonospora (Cui et al , 2001), Haloactinospora (Tang et al , 2008), Marinactinospora (Tian et al , 2009), Murinocardiopsis (Kämpfer et al , 2010), Spinactinospora (Chang et al , 2011) and Salinactinospora (Chang et al , 2012). As the largest genus in the family Nocardiopsaceae , Nocardiopsis contained 37 species and two subspecies (http://www.bacterio.net/nocardiopsis.html; Euzéby, 1997), more than half of which were isolated from saline environments such as salterns (Chun et al , 2000), saline or hypersaline soils (Al-Zarban et al , 2002; Li et al , 2003a, 2004, 2006; Hozzein et al , 2004; Zhang et al , 2008; Yang et al , 2008a; Chen et al , 2008, 2010; Hamedi et al , 2010, 2011) or marine samples (Sabry et al , 2004; Kroppenstedt & Evtushenko, 2006; Tian et al , 2009; Chen et al , 2009; Fang et al , 2011; Li et al , 2012).…”

“…In addition, one of four species in the genus Thermobifida was isolated from a salt mine (Yang et al , 2008b) and eight of nine species in the genus Streptomonospora were isolated from a salt lake or hypersaline soils (Cui et al , 2001; Li et al , 2003b; Cai et al , 2008, 2009; Meklat et al , 2014) or marine samples (Zhang et al , 2013). The genera Haloactinospora , Marinactinospora , Spinactinospora and Salinactinospora each contain only one species, and the type species of these genera were isolated from a salt lake (Tang et al , 2008) or marine sediments (Tian et al , 2009; Chang et al , 2011, 2012).…”

Culturable diversity and antimicrobial activity of Actinobacteria from marine sediments in Valparaíso bay, Chile

“…The isolates VA42-3 and VH47-3 form a separate cluster from close relatives of the genus Streptomyces (Figure 3 ). Interestingly, isolate VN6-2 exhibited 93.9% sequence similarity to Salinactinospora qingdaonensis CXB832 T , which was isolated from a salt pond in Qingdao, China (Chang et al, 2012 ). Phylogenetic analysis revealed that isolate VN6-2 forms a distinct branch together with an unpublished strain from marine sediment, actinomycete 095-35, which is well supported (Figure 3 ).…”

Actinorugispora endophytica gen. nov., sp. nov., an actinomycete isolated from Daucus carota

“…So far, there are nine genera of the family Nocardiopsaceae with validly published names: Nocardiopsis (Meyer, 1976), Thermobifida (Zhang et al , 1998), Streptomonospora (Cui et al , 2001), Haloactinospora (Tang et al , 2008), Marinactinospora (Tian et al , 2009), Murinocardiopsis (Kämpfer et al , 2010), Spinactinospora (Chang et al , 2011), Salinactinospora (Chang et al , 2012) and Allosalinactinospora (Guo et al , 2015). According to the statistics of Chang et al (2012), strains of the family Nocardiopsaceae were isolated from different environments, including hypersaline soils, marine sediments, compost, damp stored hay, manure heaps, a salt mine and salt lake. Recently, a novel strain, YIM 690008 T , was isolated from Daucus carota , a common vegetable collected from South Korea, which was found to be closely related phylogenetically to the genus Marinactinospora , but with very different phenotypic and chemotaxonomic characteristics.…”