Actinorugispora endophytica gen. nov., sp. nov., an actinomycete isolated from Daucus carota

Abstract

An actinomycete strain, designated YIM 690008T, was isolated from Daucus carota collected from South Korea and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium formed wrinkled single spores and short spore chains, some of which were branched. The whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, mannose, ribose, galactose and rhamnose. The predominant menaquinones were MK-10(H4), MK-10(H6), MK-10(H8) and MK-10(H2). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides, some unknown phospholipids, glycolipids and polar lipids. The major fatty acids were i-C16 : 0, ai-C17 : 0 and C18 : 1ω9c. The DNA G+C content of the genomic DNA was 63.1 mol%. Phylogenetic analysis indicated that the isolate belongs to the family Nocardiopsaceae. However, based on phenotypic, chemotaxonomic and genotypic data, it was concluded that strain YIM 690008T represents a novel genus and novel species of the family Nocardiopsaceae, for which the name Actinorugispora endophytica gen. nov., sp. nov. (type strain YIM 690008T = DSM 46770T = JCM 30099T = KCTC 29480T) is proposed.

Spiractinospora alimapuensis gen. nov., sp. nov., isolated from marine sediment of Valparaíso Bay (Chile) and proposal for reclassification of two species of the genus Nocardiopsis

“…The family Nocardiopsaceae was proposed by Rainey and colleagues [1] and belongs to the order Streptosporangiales [2] and the class Actinomycetia [3]. Currently, it comprises 12 genera (https://lpsn.dsmz.de/family/nocardiopsaceae), including Nocardiopsis as type genus [4], Actinorugispora [5], Allosalinactinospora [6], Haloactinospora [7], Lipingzhangella [8], Marinactinospora [9], Marinitenerispora [10], Murinocardiopsis [11], Salinactinospora [12], Spinactinospora [13], Streptomonospora [14] and Thermobifida [15]. Members of the family Nocardiopsaceae are Gram-stain-positive, aerobic and non-acid-fast bacteria.…”

“…dDDH values was calculated using the web service (http://ggdc.dsmz.de) [76] with the Table 1. Differential phenotypic and chemotaxonomic characteristics of strain VN6-2 T and closely related genera within family Nocardiopsaceae Strains: 1, VN6-2 T ; 2, Salinactinospora qingdaonensis CXB832 T [12]; 3, Murinocardiopsis flavida 14-Be-013 T [11]; 4, Lipingzhangella halophila EGI 80537 T [8]; 5, Streptomonospora arabica S186 T [20,42]; 6, Actinorugispora endophytica DSM 46770 T [5]. Cell walls of all the taxa contain meso-diaminopimelic acid.…”