Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil

Abstract

A novel actinomycete, designated strain CA15-2 T , was isolated from a soil sample collected from the rhizosphere of tamarisk in the Lop Nor region, Xinjiang, China, and was characterized by using a polyphasic taxonomic approach. Optimal growth occurred at 37 6C and pH 7.5-8.0 and with 5 % (w/v) NaCl. Strain CA15-2 T formed white to pale-yellow branched substrate mycelium without fragmentation and sparse aerial mycelium with wavelike curves. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the diagnostic diamino acid of the cell wall but no diagnostic sugars. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified glycolipid, one unidentified phospholipid and other unidentified lipids. MK-9(H 8), MK-10(H 8) and MK-10(H 6) were the predominant menaquinones. The major fatty acids were iso-C 16 : 0 and C 16 : 0. The G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CA15-2 T formed a distinct subclade in the family Nocardiopsaceae, with less than 95 % 16S rRNA gene sequence similarity to all known members of the family Nocardiopsaceae. On the basis of the evidence from our polyphasic study, a novel genus, Allosalinactinospora gen. nov., is proposed, with the type species Allosalinactinospora lopnorensis gen. nov., sp. nov. The type strain of Allosalinactinospora lopnorensis is strain CA15-2 T (5DSM 45697 T 5CGMCC 4.7074 T).

Phytoactinopolyspora mesophila sp. nov., isolated from a saline–alkaline soil

“…[14], separated and then determined by HPLC coupled with a single quadrupole mass spectrometer as described by Guo et al . [15]. Diagnostic isomers of DAP and sugar compositions in whole cell hydrolysates were prepared and determined by TLC on aluminum cellulose sheets (Merck) as described by Staneck and Roberts [16] and Hasegawa et al .…”

Description of Salilacibacter albus gen. nov., sp. nov., isolated from a dried salt lake, and reclassification of Paraglycomyces xinjiangensis Luo et al. 2015 as a later heterotypic synonym of Salininema proteolyticum Nikou et al. 2015 with emended descriptions of the genus Salininema and Salininema proteolyticum

“…), respectively. Menaquinones were extracted using the method of Collins et al (1977), and then analysed and confirmed by HPLC and a single quadrupole mass spectrometer LCMS-2020 (Shimadzu) as described by Guo et al (2015).…”

Auraticoccus cholistanensis sp. nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus

“…Analyses of polar lipids and menaquinones were performed by cultivating strains in tryptic soy broth (TSB) medium (pH 7-8) at 28 °C for 3 days in an orbital shaker. Menaquinones were purified following the previously described method [32] and analysed by HPLC/MS [33]. The polar lipids were extracted according to the method described by Minnikin et al [34] using twodimensional thin-layer chromatography (TLC) on silica gel 60 F254 plates (Merck).…”